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Alpha-synuclein immunoreactivity and ultrastructural study of glial cytoplasmic inclusions in multiple system atrophy.
Chin Med J (Engl). 2002 Oct; 115(10):1491-5.CM

Abstract

OBJECTIVE

To understand the possible pathogenesis of sporadic multiple system atrophy (MSA).

METHODS

The immunoreactivity and ultrastructural features of glial cytoplasmic inclusions (GCIs) in 12 autopsy patients with MSA and 4 normal control groups were studied. All regional sections from each subject were evaluated with HE staining, Klüver-Barrera (KB), Holzer's, modified Gallyas-Braak's (GB) methods and immunohistochemical staining with alpha-synuclein and ubiquitin antibodies. Pontine white matter with abundant GCIs from case 1 was examined, using conventional electron microscopy, Gallyas-Braak's electron microscopy and immunoelectron microscopy.

RESULTS

The presence of GCIs as constantly demonstrated in all MSA patients. Strong alpha-synuclein immunoreactivity was observed in all of the ubiquitinated GCIs. However, the density of alpha-synuclein positive GCIs differed from case to case, and there was no relationship between the density of GCIs and age, sex, or MSA subtype. Ultrastructural features indicated that argyrophilic granule-associated filaments of about 25 nm in diameter were the predominant constituents of GCIs, and the anti alpha-synuclein antibody selectively labeled in these filaments. No GCIs and alpha-synuclein immunoreaction were found in control brain tissues.

CONCLUSIONS

GCI was a pathognomonic change in sporadic MSA patients. Accumulation of alpha-synuclein in GCIs may occur during the early stags of MSA. Seletcive alpha-synuclein positive abnormal microtubules in GCIs therefore play an important role in the pathogenesis of MSA.

Authors+Show Affiliations

Wang Y
Institute of Neurology, Fudan University, Shanghai 200040, China. yinwang88@hotmail.com
Lü C
No affiliation info available
Ye Z
No affiliation info available

MeSH

AgedAged, 80 and overFemaleHumansImmunohistochemistryInclusion BodiesMaleMicroscopy, ImmunoelectronMiddle AgedMultiple System AtrophyNerve Tissue ProteinsNeurogliaSynucleinsalpha-Synuclein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12490094

Citation

Wang, Yin, et al. "Alpha-synuclein Immunoreactivity and Ultrastructural Study of Glial Cytoplasmic Inclusions in Multiple System Atrophy." Chinese Medical Journal, vol. 115, no. 10, 2002, pp. 1491-5.
Wang Y, Lü C, Ye Z. Alpha-synuclein immunoreactivity and ultrastructural study of glial cytoplasmic inclusions in multiple system atrophy. Chin Med J (Engl). 2002;115(10):1491-5.
Wang, Y., Lü, C., & Ye, Z. (2002). Alpha-synuclein immunoreactivity and ultrastructural study of glial cytoplasmic inclusions in multiple system atrophy. Chinese Medical Journal, 115(10), 1491-5.
Wang Y, Lü C, Ye Z. Alpha-synuclein Immunoreactivity and Ultrastructural Study of Glial Cytoplasmic Inclusions in Multiple System Atrophy. Chin Med J (Engl). 2002;115(10):1491-5. PubMed PMID: 12490094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alpha-synuclein immunoreactivity and ultrastructural study of glial cytoplasmic inclusions in multiple system atrophy. AU - Wang,Yin, AU - Lü,Chuanzhen, AU - Ye,Zhurong, PY - 2002/12/20/pubmed PY - 2003/1/29/medline PY - 2002/12/20/entrez SP - 1491 EP - 5 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 115 IS - 10 N2 - OBJECTIVE: To understand the possible pathogenesis of sporadic multiple system atrophy (MSA). METHODS: The immunoreactivity and ultrastructural features of glial cytoplasmic inclusions (GCIs) in 12 autopsy patients with MSA and 4 normal control groups were studied. All regional sections from each subject were evaluated with HE staining, Klüver-Barrera (KB), Holzer's, modified Gallyas-Braak's (GB) methods and immunohistochemical staining with alpha-synuclein and ubiquitin antibodies. Pontine white matter with abundant GCIs from case 1 was examined, using conventional electron microscopy, Gallyas-Braak's electron microscopy and immunoelectron microscopy. RESULTS: The presence of GCIs as constantly demonstrated in all MSA patients. Strong alpha-synuclein immunoreactivity was observed in all of the ubiquitinated GCIs. However, the density of alpha-synuclein positive GCIs differed from case to case, and there was no relationship between the density of GCIs and age, sex, or MSA subtype. Ultrastructural features indicated that argyrophilic granule-associated filaments of about 25 nm in diameter were the predominant constituents of GCIs, and the anti alpha-synuclein antibody selectively labeled in these filaments. No GCIs and alpha-synuclein immunoreaction were found in control brain tissues. CONCLUSIONS: GCI was a pathognomonic change in sporadic MSA patients. Accumulation of alpha-synuclein in GCIs may occur during the early stags of MSA. Seletcive alpha-synuclein positive abnormal microtubules in GCIs therefore play an important role in the pathogenesis of MSA. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/12490094/Alpha_synuclein_immunoreactivity_and_ultrastructural_study_of_glial_cytoplasmic_inclusions_in_multiple_system_atrophy_ L2 - https://www.diseaseinfosearch.org/result/4975 DB - PRIME DP - Unbound Medicine ER -