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[Modulation of sphingosine 1-phosphate, a new lipid mediator, on nitric oxide production by vascular smooth muscle cells].
Nihon Yakurigaku Zasshi. 2002 Nov; 120(1):70P-72P.NY

Abstract

The effects of sphingosine 1-phosphate (S1P) on nitric oxide (NO) production induced by interleukin (IL)-1 beta in cultured rat vascular smooth muscle cells (VSMCs) have been investigated. Rat VSMCs abundantly expressed mRNA of Edg-3 and Edg-5 receptor subtypes in both unstimulated and IL-1 beta-stimulated cells. S1P at higher than 0.1 microM inhibited the NO production induced by IL-1 beta in a concentration-dependent manner, in which S1P at 10 microM caused over 90% inhibition. S1P also inhibited inducible NO synthase (iNOS) protein and mRNA expressions induced by IL-1 beta in a concentration-dependent manner, which effects were smaller than that in the NO production. S1P also significantly inhibited GTP cyclohydrolase I (GTPCH) mRNA expression induced by IL-1 beta. PTX pretreatment partially prevented the inhibitory effects of S1P on the NO production and iNOS induction, while completely prevented the inhibitory effects on iNOS and GTPCH mRNA expressions. In PTX-pretreated VSMC, S1P showed a bell-shaped enhancing and inhibitory effect on iNOS protein expression. These results suggest that S1P modulates IL-1 beta induction of NO production by rat VSMCs through multiple mechanisms, partially via Edg-3 and Edg-5 receptor subtypes coupled to PTX-sensitive G proteins.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu 061-0293, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

jpn

PubMed ID

12491784

Citation

Hirafuji, Masahiko, et al. "[Modulation of Sphingosine 1-phosphate, a New Lipid Mediator, On Nitric Oxide Production By Vascular Smooth Muscle Cells]." Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica, vol. 120, no. 1, 2002, 70P-72P.
Hirafuji M, Hamaya Y, Matsumoto Y, et al. [Modulation of sphingosine 1-phosphate, a new lipid mediator, on nitric oxide production by vascular smooth muscle cells]. Nippon Yakurigaku Zasshi. 2002;120(1):70P-72P.
Hirafuji, M., Hamaya, Y., Matsumoto, Y., Machida, T., Minami, M., Kohno, T., & Igarashi, Y. (2002). [Modulation of sphingosine 1-phosphate, a new lipid mediator, on nitric oxide production by vascular smooth muscle cells]. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica, 120(1), 70P-72P.
Hirafuji M, et al. [Modulation of Sphingosine 1-phosphate, a New Lipid Mediator, On Nitric Oxide Production By Vascular Smooth Muscle Cells]. Nippon Yakurigaku Zasshi. 2002;120(1):70P-72P. PubMed PMID: 12491784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Modulation of sphingosine 1-phosphate, a new lipid mediator, on nitric oxide production by vascular smooth muscle cells]. AU - Hirafuji,Masahiko, AU - Hamaya,Yukihiro, AU - Matsumoto,Yumika, AU - Machida,Takuji, AU - Minami,Masaru, AU - Kohno,Takayuki, AU - Igarashi,Yasuyuki, PY - 2002/12/21/pubmed PY - 2003/4/2/medline PY - 2002/12/21/entrez SP - 70P EP - 72P JF - Nihon yakurigaku zasshi. Folia pharmacologica Japonica JO - Nippon Yakurigaku Zasshi VL - 120 IS - 1 N2 - The effects of sphingosine 1-phosphate (S1P) on nitric oxide (NO) production induced by interleukin (IL)-1 beta in cultured rat vascular smooth muscle cells (VSMCs) have been investigated. Rat VSMCs abundantly expressed mRNA of Edg-3 and Edg-5 receptor subtypes in both unstimulated and IL-1 beta-stimulated cells. S1P at higher than 0.1 microM inhibited the NO production induced by IL-1 beta in a concentration-dependent manner, in which S1P at 10 microM caused over 90% inhibition. S1P also inhibited inducible NO synthase (iNOS) protein and mRNA expressions induced by IL-1 beta in a concentration-dependent manner, which effects were smaller than that in the NO production. S1P also significantly inhibited GTP cyclohydrolase I (GTPCH) mRNA expression induced by IL-1 beta. PTX pretreatment partially prevented the inhibitory effects of S1P on the NO production and iNOS induction, while completely prevented the inhibitory effects on iNOS and GTPCH mRNA expressions. In PTX-pretreated VSMC, S1P showed a bell-shaped enhancing and inhibitory effect on iNOS protein expression. These results suggest that S1P modulates IL-1 beta induction of NO production by rat VSMCs through multiple mechanisms, partially via Edg-3 and Edg-5 receptor subtypes coupled to PTX-sensitive G proteins. SN - 0015-5691 UR - https://www.unboundmedicine.com/medline/citation/12491784/[Modulation_of_sphingosine_1_phosphate_a_new_lipid_mediator_on_nitric_oxide_production_by_vascular_smooth_muscle_cells]_ L2 - http://www.medicalonline.jp/meteo_linkout.php?issn=0015-5691&volume=120&issue=1&spage=70P DB - PRIME DP - Unbound Medicine ER -