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Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects.
Br J Nutr. 2002 Dec; 88(6):615-23.BJ

Abstract

The present study investigated whether storage under modified-atmosphere packaging (MAP) affected the antioxidant properties of fresh lettuce (Lactuca sativa). Eleven healthy volunteers (six men, five women) consumed 250 g fresh lettuce, and blood was sampled before (0 h) and 2, 3 and 6 h after consumption. The protocol was repeated 3 d later with the same lettuce stored at 5 degrees C under MAP conditions (O2-N2 (5:95, v/v)). Results showed that after ingestion of fresh lettuce, plasma total radical-trapping antioxidant potential (TRAP), measured as area under the curve, was significantly higher (1.3 (sem 0.3) mmol/l per 6 h; P<0.05) than the value obtained with MAP-stored lettuce (0.1 (sem 0.2) mmol/l per 6 h). Plasma TRAP, quercetin and p-coumaric acid were significantly different from baseline values (P<or=0.05) 2 and 3 h after fresh lettuce ingestion. Caffeic acid increased significantly at 3 h (P<0.05). Plasma beta-carotene levels increased significantly at 6 h (P<0.05). Vitamin C concentrations (mg/l) rose from 10.9 (sem 2.0) to 12.7 (sem 3.0) (P<0.001), 12.7 (sem 2.0) (P<0.01) and 12.9 (sem 3.0) (P<0.05) at 0, 2, 3 and 6 h respectively. No changes were observed after ingestion of MAP-stored lettuce for all the measured markers. Our present results showed that ingestion of MAP-stored lettuce does not modify plasma redox status in healthy subjects. Further research is needed to develop post-harvesting techniques able to preserve the bioactive molecule content of plant food.

Authors+Show Affiliations

Serafini M
Antioxidant Research Laboratory at the Unit of Human Nutrition, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy. serafini@inran.it
Bugianesi R
No affiliation info available
Salucci M
No affiliation info available
Azzini E
No affiliation info available
Raguzzini A
No affiliation info available
Maiani G
No affiliation info available

MeSH

AdultAnalysis of VarianceAntioxidantsArea Under CurveAscorbic AcidBiological AvailabilityCaffeic AcidsCarotenoidsCoumaric AcidsEatingFemaleFlavonoidsFood HandlingFood PackagingFree RadicalsHumansLettuceMaleMiddle AgedOxidation-ReductionPhenolsPolymersQuercetinTime Factors

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12493083

Citation

Serafini, Mauro, et al. "Effect of Acute Ingestion of Fresh and Stored Lettuce (Lactuca Sativa) On Plasma Total Antioxidant Capacity and Antioxidant Levels in Human Subjects." The British Journal of Nutrition, vol. 88, no. 6, 2002, pp. 615-23.
Serafini M, Bugianesi R, Salucci M, et al. Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects. Br J Nutr. 2002;88(6):615-23.
Serafini, M., Bugianesi, R., Salucci, M., Azzini, E., Raguzzini, A., & Maiani, G. (2002). Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects. The British Journal of Nutrition, 88(6), 615-23.
Serafini M, et al. Effect of Acute Ingestion of Fresh and Stored Lettuce (Lactuca Sativa) On Plasma Total Antioxidant Capacity and Antioxidant Levels in Human Subjects. Br J Nutr. 2002;88(6):615-23. PubMed PMID: 12493083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects. AU - Serafini,Mauro, AU - Bugianesi,Rossana, AU - Salucci,Monica, AU - Azzini,Elena, AU - Raguzzini,Anna, AU - Maiani,Giuseppe, PY - 2002/12/21/pubmed PY - 2003/1/29/medline PY - 2002/12/21/entrez SP - 615 EP - 23 JF - The British journal of nutrition JO - Br J Nutr VL - 88 IS - 6 N2 - The present study investigated whether storage under modified-atmosphere packaging (MAP) affected the antioxidant properties of fresh lettuce (Lactuca sativa). Eleven healthy volunteers (six men, five women) consumed 250 g fresh lettuce, and blood was sampled before (0 h) and 2, 3 and 6 h after consumption. The protocol was repeated 3 d later with the same lettuce stored at 5 degrees C under MAP conditions (O2-N2 (5:95, v/v)). Results showed that after ingestion of fresh lettuce, plasma total radical-trapping antioxidant potential (TRAP), measured as area under the curve, was significantly higher (1.3 (sem 0.3) mmol/l per 6 h; P<0.05) than the value obtained with MAP-stored lettuce (0.1 (sem 0.2) mmol/l per 6 h). Plasma TRAP, quercetin and p-coumaric acid were significantly different from baseline values (P<or=0.05) 2 and 3 h after fresh lettuce ingestion. Caffeic acid increased significantly at 3 h (P<0.05). Plasma beta-carotene levels increased significantly at 6 h (P<0.05). Vitamin C concentrations (mg/l) rose from 10.9 (sem 2.0) to 12.7 (sem 3.0) (P<0.001), 12.7 (sem 2.0) (P<0.01) and 12.9 (sem 3.0) (P<0.05) at 0, 2, 3 and 6 h respectively. No changes were observed after ingestion of MAP-stored lettuce for all the measured markers. Our present results showed that ingestion of MAP-stored lettuce does not modify plasma redox status in healthy subjects. Further research is needed to develop post-harvesting techniques able to preserve the bioactive molecule content of plant food. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/12493083/Effect_of_acute_ingestion_of_fresh_and_stored_lettuce__Lactuca_sativa__on_plasma_total_antioxidant_capacity_and_antioxidant_levels_in_human_subjects_ DB - PRIME DP - Unbound Medicine ER -