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Sulfated and pyruvylated disaccharide alditols obtained from a red seaweed galactan: ESIMS and NMR approaches.
Carbohydr Res. 2002 Nov 29; 337(24):2443-53.CR

Abstract

The water-soluble acid agaran isolated from Acanthophora spicifera (Rhodophyta) was submitted to alkaline treatment for the complete cyclization of alpha-L-Galp 6-sulfate to 3,6-An-alpha-L-Galp units. The modified agaran was then partially depolymerized using partial reductive hydrolysis. The resulting oligosaccharide mixture was fractionated by adsorption and ion-exchange chromatography. Fractions were purified by gel-filtration chromatography and studied by ESIMS and NMR spectroscopy, including 1D 1H, 13C, DEPT and 2D 1H, 1H COSY, TOCSY and 1H, 13C HMQC procedures. The following neutral, pyruvylated, sulfated and sulfated/pyruvylated disaccharide alditols were obtained: beta-D-Galp-(1-->4)-3,6-An-L-GalOH; 4,6-O-(1-carboxyethylidene)-beta-D-Galp-(1-->4)-3,6-An-L-GalOH; beta-D-Galp 2-sulfate-(1-->4)-3,6-An-L-GalOH and 4,6-O-(1-carboxyethylidene)-beta-D-Galp 2-sulfate-(1-->4)-3,6-An-L-GalOH.

Authors+Show Affiliations

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12493229

Citation

Gonçalves, Alan G., et al. "Sulfated and Pyruvylated Disaccharide Alditols Obtained From a Red Seaweed Galactan: ESIMS and NMR Approaches." Carbohydrate Research, vol. 337, no. 24, 2002, pp. 2443-53.
Gonçalves AG, Ducatti DR, Duarte ME, et al. Sulfated and pyruvylated disaccharide alditols obtained from a red seaweed galactan: ESIMS and NMR approaches. Carbohydr Res. 2002;337(24):2443-53.
Gonçalves, A. G., Ducatti, D. R., Duarte, M. E., & Noseda, M. D. (2002). Sulfated and pyruvylated disaccharide alditols obtained from a red seaweed galactan: ESIMS and NMR approaches. Carbohydrate Research, 337(24), 2443-53.
Gonçalves AG, et al. Sulfated and Pyruvylated Disaccharide Alditols Obtained From a Red Seaweed Galactan: ESIMS and NMR Approaches. Carbohydr Res. 2002 Nov 29;337(24):2443-53. PubMed PMID: 12493229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sulfated and pyruvylated disaccharide alditols obtained from a red seaweed galactan: ESIMS and NMR approaches. AU - Gonçalves,Alan G, AU - Ducatti,Diogo R B, AU - Duarte,M Eugênia R, AU - Noseda,Miguel D, PY - 2002/12/21/pubmed PY - 2003/7/10/medline PY - 2002/12/21/entrez SP - 2443 EP - 53 JF - Carbohydrate research JO - Carbohydr Res VL - 337 IS - 24 N2 - The water-soluble acid agaran isolated from Acanthophora spicifera (Rhodophyta) was submitted to alkaline treatment for the complete cyclization of alpha-L-Galp 6-sulfate to 3,6-An-alpha-L-Galp units. The modified agaran was then partially depolymerized using partial reductive hydrolysis. The resulting oligosaccharide mixture was fractionated by adsorption and ion-exchange chromatography. Fractions were purified by gel-filtration chromatography and studied by ESIMS and NMR spectroscopy, including 1D 1H, 13C, DEPT and 2D 1H, 1H COSY, TOCSY and 1H, 13C HMQC procedures. The following neutral, pyruvylated, sulfated and sulfated/pyruvylated disaccharide alditols were obtained: beta-D-Galp-(1-->4)-3,6-An-L-GalOH; 4,6-O-(1-carboxyethylidene)-beta-D-Galp-(1-->4)-3,6-An-L-GalOH; beta-D-Galp 2-sulfate-(1-->4)-3,6-An-L-GalOH and 4,6-O-(1-carboxyethylidene)-beta-D-Galp 2-sulfate-(1-->4)-3,6-An-L-GalOH. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/12493229/Sulfated_and_pyruvylated_disaccharide_alditols_obtained_from_a_red_seaweed_galactan:_ESIMS_and_NMR_approaches_ DB - PRIME DP - Unbound Medicine ER -