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Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.
Cytometry. 2002 Dec 15; 50(6):291-7.C

Abstract

BACKGROUND

Characteristic changes in the proportions of leukocyte populations in bronchoalveolar lavage (BAL) reflect different disease states in the lung. The standard method for examination of BAL leukocytes is by microscopy of cytospin preparations. This method may not be the optimum technique due to difficulties in distinguishing cell types morphologically and due to the low number of cells routinely counted. We hypothesized that flow cytometry (FCM) may be a more precise tool for investigating BAL.

METHODS

100 BALs were performed on 92 patients. All samples were stained using the pan-leukocyte marker (CD45) in combination with a granulocyte marker (CD15) and a cell viability marker (7-aminoactinomycin D). Selected samples were also stained with an eosinophil marker (CD23). These samples were run on an FCM and the results compared with leukocyte differentials obtained by light microscopy of parallel cytospin preparations.

RESULTS

Close correlations between the two methods were demonstrated for the enumeration of all leukocyte subsets, but the coefficient of variation was considerably lower by FCM than by cytospin.

CONCLUSIONS

These findings, combined with the speed of FCM and the ability to perform simple lymphocyte phenotyping, argue in favor of this becoming the method of choice for investigating BAL.

Authors+Show Affiliations

Department of Clinical Immunology, Royal Free Hospital, London, England, UK. sbarry@doctors.org.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12497590

Citation

Barry, Simon M., et al. "Determination of Bronchoalveolar Lavage Leukocyte Populations By Flow Cytometry in Patients Investigated for Respiratory Disease." Cytometry, vol. 50, no. 6, 2002, pp. 291-7.
Barry SM, Condez A, Johnson MA, et al. Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease. Cytometry. 2002;50(6):291-7.
Barry, S. M., Condez, A., Johnson, M. A., & Janossy, G. (2002). Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease. Cytometry, 50(6), 291-7.
Barry SM, et al. Determination of Bronchoalveolar Lavage Leukocyte Populations By Flow Cytometry in Patients Investigated for Respiratory Disease. Cytometry. 2002 Dec 15;50(6):291-7. PubMed PMID: 12497590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease. AU - Barry,Simon M, AU - Condez,Aida, AU - Johnson,Margaret A, AU - Janossy,George, PY - 2002/12/24/pubmed PY - 2003/6/5/medline PY - 2002/12/24/entrez SP - 291 EP - 7 JF - Cytometry JO - Cytometry VL - 50 IS - 6 N2 - BACKGROUND: Characteristic changes in the proportions of leukocyte populations in bronchoalveolar lavage (BAL) reflect different disease states in the lung. The standard method for examination of BAL leukocytes is by microscopy of cytospin preparations. This method may not be the optimum technique due to difficulties in distinguishing cell types morphologically and due to the low number of cells routinely counted. We hypothesized that flow cytometry (FCM) may be a more precise tool for investigating BAL. METHODS: 100 BALs were performed on 92 patients. All samples were stained using the pan-leukocyte marker (CD45) in combination with a granulocyte marker (CD15) and a cell viability marker (7-aminoactinomycin D). Selected samples were also stained with an eosinophil marker (CD23). These samples were run on an FCM and the results compared with leukocyte differentials obtained by light microscopy of parallel cytospin preparations. RESULTS: Close correlations between the two methods were demonstrated for the enumeration of all leukocyte subsets, but the coefficient of variation was considerably lower by FCM than by cytospin. CONCLUSIONS: These findings, combined with the speed of FCM and the ability to perform simple lymphocyte phenotyping, argue in favor of this becoming the method of choice for investigating BAL. SN - 0196-4763 UR - https://www.unboundmedicine.com/medline/citation/12497590/Determination_of_bronchoalveolar_lavage_leukocyte_populations_by_flow_cytometry_in_patients_investigated_for_respiratory_disease_ L2 - https://doi.org/10.1002/cyto.10151 DB - PRIME DP - Unbound Medicine ER -