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Analysis of illicit amphetamine seizures by capillary zone electrophoresis.
J Chromatogr A. 2002 Dec 06; 979(1-2):345-52.JC

Abstract

Capillary zone electrophoresis was applied for the determination of amphetamine and related substances in seized drugs. A buffer made of 0.1 M phosphoric acid adjusted to pH 3.0 with triethanolamine was selected. With this background electrolyte, triethanolamine is adsorbed to the capillary wall and the electroosmotic flow is reversed. This gives rise to peaks with good symmetry, high efficiency and reproducible migration times. The separation of the different analytes was performed in a fused-silica capillary thermostatted at 25 degrees C and the applied voltage was 25 kV. Under these experimental conditions, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethamphetamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butamine and ephedrine were resolved within 8 min and without interference from adulterants usually found in illicit powders. Their identification by the migration time was confirmed by their UV spectra recorded with a diode array UV detector (190-350 nm). The selected method was then applied to identify these substances in illicit tablets known as "Ecstasy" and the MDMA determined in these samples according to a laboratory validation procedure.

Authors+Show Affiliations

Laboratory of Drug Analysis, Scientific Institute of Public Health-Louis Pasteur, Rue Juliette Wytsman 14, B-1050 Brussels, Belgium. veronique.piette@iph.fgov.beNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12498266

Citation

Piette, Véronique, and Frans Parmentier. "Analysis of Illicit Amphetamine Seizures By Capillary Zone Electrophoresis." Journal of Chromatography. A, vol. 979, no. 1-2, 2002, pp. 345-52.
Piette V, Parmentier F. Analysis of illicit amphetamine seizures by capillary zone electrophoresis. J Chromatogr A. 2002;979(1-2):345-52.
Piette, V., & Parmentier, F. (2002). Analysis of illicit amphetamine seizures by capillary zone electrophoresis. Journal of Chromatography. A, 979(1-2), 345-52.
Piette V, Parmentier F. Analysis of Illicit Amphetamine Seizures By Capillary Zone Electrophoresis. J Chromatogr A. 2002 Dec 6;979(1-2):345-52. PubMed PMID: 12498266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of illicit amphetamine seizures by capillary zone electrophoresis. AU - Piette,Véronique, AU - Parmentier,Frans, PY - 2002/12/25/pubmed PY - 2003/5/15/medline PY - 2002/12/25/entrez SP - 345 EP - 52 JF - Journal of chromatography. A JO - J Chromatogr A VL - 979 IS - 1-2 N2 - Capillary zone electrophoresis was applied for the determination of amphetamine and related substances in seized drugs. A buffer made of 0.1 M phosphoric acid adjusted to pH 3.0 with triethanolamine was selected. With this background electrolyte, triethanolamine is adsorbed to the capillary wall and the electroosmotic flow is reversed. This gives rise to peaks with good symmetry, high efficiency and reproducible migration times. The separation of the different analytes was performed in a fused-silica capillary thermostatted at 25 degrees C and the applied voltage was 25 kV. Under these experimental conditions, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethamphetamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butamine and ephedrine were resolved within 8 min and without interference from adulterants usually found in illicit powders. Their identification by the migration time was confirmed by their UV spectra recorded with a diode array UV detector (190-350 nm). The selected method was then applied to identify these substances in illicit tablets known as "Ecstasy" and the MDMA determined in these samples according to a laboratory validation procedure. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/12498266/Analysis_of_illicit_amphetamine_seizures_by_capillary_zone_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(02)01405-X DB - PRIME DP - Unbound Medicine ER -