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Effects of octreotide acetate and Saccharomyces boulardii on bacterial translocation in an experimental intestinal loop obstruction model of rats.
Tohoku J Exp Med. 2002 Sep; 198(1):1-9.TJ

Abstract

Intestinal obstruction (IO) induces bacterial translocation (BT) due to mucosal disruption, motility dysfunction, and increased intestinal volume, leading to bacterial overgrowth. This study was conducted to investigate the effects of octreotide acetate (OA) and Saccharomyces boulardii (SB) on the BT and intestinal integrity in an animal model of intestinal loop obstruction (LO). Forty adult male Sprague-Dawley rats (250-300 g) were randomized into 4 groups containing 10 rats each. Complete IO was created in the distal ileum of rats by a single 3-0 silk suture (LO). Group Sham: Sham (Laparotomy only was performed in this group); group LO: LO; group OA: LO plus OA (100 microg/kg, at 0, 12 hours of obstruction); group (SB): LO plus SB (800 mg/kg/day, via orogastric and preoperative for 3 days). After 24 hours, samples of mesenteric lymph nodes (MLN), liver, spleen and blood were obtained and cultured. The terminal ileum specimens were examined histopathologically. There were no BT in group Sham, but BT was noticed totally in 31 (77.5%) cultures in group LO. This rate was reduced to 30% (n = 12), 10% (n = 4) in the groups OA and SB respectively. Bacterial translocations of MLN and the liver in group LO were significantly higher than those of groups OA and SB. Bacterial translocations of the both spleen and blood in group LO were significantly higher than those of groups OA and SB. The mean bacterial counts, colony-forming units per gram tissue (cfu/g), in the MLN, liver and spleen of group LO were found significantly higher than those of groups OA and SB. The mean villus height in group OA was significantly higher than that of group LO and it in the group SB significantly higher than those of groups LO and OA. The present experimental study has demonstrated that OA and SB may have protective effects against BT in mechanical bowel obstruction and additionally SB preserves intestinal mucosal integrity.

Authors+Show Affiliations

Department of General Surgery, Dicle University, Faculty of Medicine, Diyarbakir, Turkey. maldemir21@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12498309

Citation

Aldemir, Mustafa, et al. "Effects of Octreotide Acetate and Saccharomyces Boulardii On Bacterial Translocation in an Experimental Intestinal Loop Obstruction Model of Rats." The Tohoku Journal of Experimental Medicine, vol. 198, no. 1, 2002, pp. 1-9.
Aldemir M, Kökoğlu OF, Geyik MF, et al. Effects of octreotide acetate and Saccharomyces boulardii on bacterial translocation in an experimental intestinal loop obstruction model of rats. Tohoku J Exp Med. 2002;198(1):1-9.
Aldemir, M., Kökoğlu, O. F., Geyik, M. F., & Büyükbayram, H. (2002). Effects of octreotide acetate and Saccharomyces boulardii on bacterial translocation in an experimental intestinal loop obstruction model of rats. The Tohoku Journal of Experimental Medicine, 198(1), 1-9.
Aldemir M, et al. Effects of Octreotide Acetate and Saccharomyces Boulardii On Bacterial Translocation in an Experimental Intestinal Loop Obstruction Model of Rats. Tohoku J Exp Med. 2002;198(1):1-9. PubMed PMID: 12498309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of octreotide acetate and Saccharomyces boulardii on bacterial translocation in an experimental intestinal loop obstruction model of rats. AU - Aldemir,Mustafa, AU - Kökoğlu,O Faruk, AU - Geyik,M Faruk, AU - Büyükbayram,Hüseyin, PY - 2002/12/25/pubmed PY - 2003/5/21/medline PY - 2002/12/25/entrez SP - 1 EP - 9 JF - The Tohoku journal of experimental medicine JO - Tohoku J Exp Med VL - 198 IS - 1 N2 - Intestinal obstruction (IO) induces bacterial translocation (BT) due to mucosal disruption, motility dysfunction, and increased intestinal volume, leading to bacterial overgrowth. This study was conducted to investigate the effects of octreotide acetate (OA) and Saccharomyces boulardii (SB) on the BT and intestinal integrity in an animal model of intestinal loop obstruction (LO). Forty adult male Sprague-Dawley rats (250-300 g) were randomized into 4 groups containing 10 rats each. Complete IO was created in the distal ileum of rats by a single 3-0 silk suture (LO). Group Sham: Sham (Laparotomy only was performed in this group); group LO: LO; group OA: LO plus OA (100 microg/kg, at 0, 12 hours of obstruction); group (SB): LO plus SB (800 mg/kg/day, via orogastric and preoperative for 3 days). After 24 hours, samples of mesenteric lymph nodes (MLN), liver, spleen and blood were obtained and cultured. The terminal ileum specimens were examined histopathologically. There were no BT in group Sham, but BT was noticed totally in 31 (77.5%) cultures in group LO. This rate was reduced to 30% (n = 12), 10% (n = 4) in the groups OA and SB respectively. Bacterial translocations of MLN and the liver in group LO were significantly higher than those of groups OA and SB. Bacterial translocations of the both spleen and blood in group LO were significantly higher than those of groups OA and SB. The mean bacterial counts, colony-forming units per gram tissue (cfu/g), in the MLN, liver and spleen of group LO were found significantly higher than those of groups OA and SB. The mean villus height in group OA was significantly higher than that of group LO and it in the group SB significantly higher than those of groups LO and OA. The present experimental study has demonstrated that OA and SB may have protective effects against BT in mechanical bowel obstruction and additionally SB preserves intestinal mucosal integrity. SN - 0040-8727 UR - https://www.unboundmedicine.com/medline/citation/12498309/Effects_of_octreotide_acetate_and_Saccharomyces_boulardii_on_bacterial_translocation_in_an_experimental_intestinal_loop_obstruction_model_of_rats_ L2 - https://japanlinkcenter.org/JST.JSTAGE/tjem/198.1?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -