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Six and Eya expression during human somitogenesis and MyoD gene family activation.
J Muscle Res Cell Motil. 2002; 23(3):255-64.JM

Abstract

This report describes the characterisation of the expression profile of several myogenic determination genes during human embryogenesis. The data were obtained from axial structures and limb buds of human embryos aged between 3 and 8 weeks of development. Using in situ hybridisation to detect Pax3 and MyoD gene family mRNAs, and immunochemistry to follow Six and Eya protein accumulation, we have been able to establish the chronology of accumulation of these gene products. As in mouse, the first transcripts detected in myotomes of 3 week-old embryos are Pax3 and Myf5, followed by the expression of myogenin. MyoD appears to be activated well after Myf5, myogenin and MRF4 in the early myotome, whereas, in limb bud muscles, the presence of all four of these mRNAs is concomitant from 6 weeks. Six1, Six4 and Six5 homeoproteins are detected later than Myf5 activation. These Six homeoproteins are first observed in the cytoplasm of myogenin expressing cells. At later stages of development, Six1 and Six5, but not Six4, are translocated into the nuclei of myogenic cells, concomitantly with MyHCemb expression. Eya1 and Eya2 proteins, potential Six cofactors, were also detected in myogenin positive cells, but their accumulation was delayed and was mainly cytoplasmic. These results preclude that early activation of Myf5, myogenin and MRF4 is under the control of Six and Eya proteins, while Six and Eya proteins would be involved in later steps of myogenic differentiation.

Authors+Show Affiliations

URA CNRS 1923, Généthon, 1 rue de l'Internationale, BP60, 91002 Evry, France. fougerou@genethon.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12500905

Citation

Fougerousse, Françoise, et al. "Six and Eya Expression During Human Somitogenesis and MyoD Gene Family Activation." Journal of Muscle Research and Cell Motility, vol. 23, no. 3, 2002, pp. 255-64.
Fougerousse F, Durand M, Lopez S, et al. Six and Eya expression during human somitogenesis and MyoD gene family activation. J Muscle Res Cell Motil. 2002;23(3):255-64.
Fougerousse, F., Durand, M., Lopez, S., Suel, L., Demignon, J., Thornton, C., Ozaki, H., Kawakami, K., Barbet, P., Beckmann, J. S., & Maire, P. (2002). Six and Eya expression during human somitogenesis and MyoD gene family activation. Journal of Muscle Research and Cell Motility, 23(3), 255-64.
Fougerousse F, et al. Six and Eya Expression During Human Somitogenesis and MyoD Gene Family Activation. J Muscle Res Cell Motil. 2002;23(3):255-64. PubMed PMID: 12500905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Six and Eya expression during human somitogenesis and MyoD gene family activation. AU - Fougerousse,Françoise, AU - Durand,Muriel, AU - Lopez,Soledad, AU - Suel,Laurence, AU - Demignon,Josiane, AU - Thornton,Charles, AU - Ozaki,Hidenori, AU - Kawakami,Kyoshi, AU - Barbet,Patrick, AU - Beckmann,Jacques S, AU - Maire,Pascal, PY - 2002/12/26/pubmed PY - 2003/5/31/medline PY - 2002/12/26/entrez SP - 255 EP - 64 JF - Journal of muscle research and cell motility JO - J. Muscle Res. Cell. Motil. VL - 23 IS - 3 N2 - This report describes the characterisation of the expression profile of several myogenic determination genes during human embryogenesis. The data were obtained from axial structures and limb buds of human embryos aged between 3 and 8 weeks of development. Using in situ hybridisation to detect Pax3 and MyoD gene family mRNAs, and immunochemistry to follow Six and Eya protein accumulation, we have been able to establish the chronology of accumulation of these gene products. As in mouse, the first transcripts detected in myotomes of 3 week-old embryos are Pax3 and Myf5, followed by the expression of myogenin. MyoD appears to be activated well after Myf5, myogenin and MRF4 in the early myotome, whereas, in limb bud muscles, the presence of all four of these mRNAs is concomitant from 6 weeks. Six1, Six4 and Six5 homeoproteins are detected later than Myf5 activation. These Six homeoproteins are first observed in the cytoplasm of myogenin expressing cells. At later stages of development, Six1 and Six5, but not Six4, are translocated into the nuclei of myogenic cells, concomitantly with MyHCemb expression. Eya1 and Eya2 proteins, potential Six cofactors, were also detected in myogenin positive cells, but their accumulation was delayed and was mainly cytoplasmic. These results preclude that early activation of Myf5, myogenin and MRF4 is under the control of Six and Eya proteins, while Six and Eya proteins would be involved in later steps of myogenic differentiation. SN - 0142-4319 UR - https://www.unboundmedicine.com/medline/citation/12500905/Six_and_Eya_expression_during_human_somitogenesis_and_MyoD_gene_family_activation_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=12500905.ui DB - PRIME DP - Unbound Medicine ER -