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Application of the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay to a flow injection system for the evaluation of antioxidant activity of some pure compounds and beverages.
J Agric Food Chem 2003; 51(1):260-4JA

Abstract

The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(*)(+)) assay was adapted to a flow injection (FI) system to obtain a sensitive and rapid technique for the monitoring of antioxidant activity of pure compounds and complex matrixes, such as beverages and food extracts. The FI system includes a HPLC pump that flows the mobile phase (a solution of ABTS(*)(+) in ethanol) through a 20 microL loop injector, a single bead string reactor filled with acid-washed silanized beads, a delay coil and a photodiode array UV-visible detector. The technique was very sensitive, with limits of detection and of quantification of 4.14 and 9.29 micromol of Trolox/L, respectively, and demonstrated high repeatability and reproducibility. The proposed technique was then applied to the evaluation of the antioxidant activity of some pure compounds, demonstrating good agreement with published data obtained by the original spectrophotometric ABTS(*)(+) assay. Finally, the total antioxidant activity of 10 beverages was determined by both the proposed and the original method. The values ranged from 0.09 mmol L(-)(1) for cola to 49.24 mmol L(-)(1) for espresso coffee and did not result significantly different from those obtained by the original spectrophotometric ABTS(*)(+) assay (Student's paired t-test: t = 1.4074, p = 0.1929). In conclusion, the proposed FI technique seems suitable for the direct, rapid and reliable monitoring of total antioxidant activity of pure compounds and beverages and, due to the ability to operate in continuous, it allows the analysis of about 30 samples h(-)(1) making the assay particularly suitable for large screening of total antioxidant activity in food samples.

Authors+Show Affiliations

Department of Public Health, University of Parma, Via Volturno, 39, Italy. mailnico@nemo.unipr.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12502418

Citation

Pellegrini, Nicoletta, et al. "Application of the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic Acid) Radical Cation Assay to a Flow Injection System for the Evaluation of Antioxidant Activity of some Pure Compounds and Beverages." Journal of Agricultural and Food Chemistry, vol. 51, no. 1, 2003, pp. 260-4.
Pellegrini N, Del Rio D, Colombi B, et al. Application of the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay to a flow injection system for the evaluation of antioxidant activity of some pure compounds and beverages. J Agric Food Chem. 2003;51(1):260-4.
Pellegrini, N., Del Rio, D., Colombi, B., Bianchi, M., & Brighenti, F. (2003). Application of the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay to a flow injection system for the evaluation of antioxidant activity of some pure compounds and beverages. Journal of Agricultural and Food Chemistry, 51(1), pp. 260-4.
Pellegrini N, et al. Application of the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic Acid) Radical Cation Assay to a Flow Injection System for the Evaluation of Antioxidant Activity of some Pure Compounds and Beverages. J Agric Food Chem. 2003 Jan 1;51(1):260-4. PubMed PMID: 12502418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay to a flow injection system for the evaluation of antioxidant activity of some pure compounds and beverages. AU - Pellegrini,Nicoletta, AU - Del Rio,Daniele, AU - Colombi,Barbara, AU - Bianchi,Marta, AU - Brighenti,Furio, PY - 2002/12/28/pubmed PY - 2003/2/22/medline PY - 2002/12/28/entrez SP - 260 EP - 4 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 51 IS - 1 N2 - The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(*)(+)) assay was adapted to a flow injection (FI) system to obtain a sensitive and rapid technique for the monitoring of antioxidant activity of pure compounds and complex matrixes, such as beverages and food extracts. The FI system includes a HPLC pump that flows the mobile phase (a solution of ABTS(*)(+) in ethanol) through a 20 microL loop injector, a single bead string reactor filled with acid-washed silanized beads, a delay coil and a photodiode array UV-visible detector. The technique was very sensitive, with limits of detection and of quantification of 4.14 and 9.29 micromol of Trolox/L, respectively, and demonstrated high repeatability and reproducibility. The proposed technique was then applied to the evaluation of the antioxidant activity of some pure compounds, demonstrating good agreement with published data obtained by the original spectrophotometric ABTS(*)(+) assay. Finally, the total antioxidant activity of 10 beverages was determined by both the proposed and the original method. The values ranged from 0.09 mmol L(-)(1) for cola to 49.24 mmol L(-)(1) for espresso coffee and did not result significantly different from those obtained by the original spectrophotometric ABTS(*)(+) assay (Student's paired t-test: t = 1.4074, p = 0.1929). In conclusion, the proposed FI technique seems suitable for the direct, rapid and reliable monitoring of total antioxidant activity of pure compounds and beverages and, due to the ability to operate in continuous, it allows the analysis of about 30 samples h(-)(1) making the assay particularly suitable for large screening of total antioxidant activity in food samples. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12502418/Application_of_the_22'_azinobis_3_ethylbenzothiazoline_6_sulfonic_acid__radical_cation_assay_to_a_flow_injection_system_for_the_evaluation_of_antioxidant_activity_of_some_pure_compounds_and_beverages_ L2 - https://dx.doi.org/10.1021/jf020657z DB - PRIME DP - Unbound Medicine ER -