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Effect of transforming growth factor-alpha on intestinal adaptation in a rat model of short bowel syndrome.
J Surg Res. 2002 Dec; 108(2):235-42.JS

Abstract

OBJECTIVE

TGF-alpha has recently been shown to stimulate enterocyte proliferation. In the present study we investigated the effect of TGF-alpha on enterocyte proliferation and loss via apoptosis and its effects on intestinal adaptation in a rat following massive bowel resection.

METHODS

Male Sprague-Dawley rats underwent bowel transection and reanastomosis (sham group) or 75% small bowel resection and anastomosis (SBS group) and were treated with intraperitoneal TGF-alpha (75 microg/kg) from the ninth postoperative day (SBS-TGF-alpha group). Parameters of intestinal adaptation (overall bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth), enterocyte proliferation, and apoptosis were determined on day 15. Statistical significance was determined by ANOVA with a P < 0.05 considered significant.

RESULTS

SBS-TGF-alpha rats demonstrated a significant increase (vs SBS) in duodenal, jejunal, and ileal overall bowel and mucosal weights; ileal mucosal DNA and protein; and jejunal and ileal villus height. SBS-TGF-alpha rats also showed an increased cell proliferation index in jejunum (704 +/- 43 vs 499 +/- 63 BrdU-positive cells/10 crypts, P < 0.05) and ileum (715 +/- 84 vs 529 +/- 40 BrdU-positive cells/10 crypts, P < 0.05) and decreased apoptotic index in ileum (8.7 +/- 1.1 vs 21.8 +/- 3.2 apoptotic cells/1,000 villus cells, P < 0.05) compared to SBS animals.

CONCLUSIONS

In a rat model of SBS, TGF-alpha enhances intestinal adaptation. Possible mechanisms may include increased cell proliferation and decreased enterocyte loss via apoptosis.

Authors+Show Affiliations

Rappaport Faculty of Medicine, Technion, Carmel Medical Center, Haifa 34362, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12505047

Citation

Sukhotnik, Igor, et al. "Effect of Transforming Growth Factor-alpha On Intestinal Adaptation in a Rat Model of Short Bowel Syndrome." The Journal of Surgical Research, vol. 108, no. 2, 2002, pp. 235-42.
Sukhotnik I, Yakirevich E, Coran AG, et al. Effect of transforming growth factor-alpha on intestinal adaptation in a rat model of short bowel syndrome. J Surg Res. 2002;108(2):235-42.
Sukhotnik, I., Yakirevich, E., Coran, A. G., Siplovich, L., Krausz, M., Hirsh, M., Sabo, E., & Shiloni, E. (2002). Effect of transforming growth factor-alpha on intestinal adaptation in a rat model of short bowel syndrome. The Journal of Surgical Research, 108(2), 235-42.
Sukhotnik I, et al. Effect of Transforming Growth Factor-alpha On Intestinal Adaptation in a Rat Model of Short Bowel Syndrome. J Surg Res. 2002;108(2):235-42. PubMed PMID: 12505047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of transforming growth factor-alpha on intestinal adaptation in a rat model of short bowel syndrome. AU - Sukhotnik,Igor, AU - Yakirevich,Evgeny, AU - Coran,Arnold G, AU - Siplovich,Leonardo, AU - Krausz,Michael, AU - Hirsh,Mark, AU - Sabo,Edmund, AU - Shiloni,Eitan, PY - 2002/12/31/pubmed PY - 2003/1/23/medline PY - 2002/12/31/entrez SP - 235 EP - 42 JF - The Journal of surgical research JO - J Surg Res VL - 108 IS - 2 N2 - OBJECTIVE: TGF-alpha has recently been shown to stimulate enterocyte proliferation. In the present study we investigated the effect of TGF-alpha on enterocyte proliferation and loss via apoptosis and its effects on intestinal adaptation in a rat following massive bowel resection. METHODS: Male Sprague-Dawley rats underwent bowel transection and reanastomosis (sham group) or 75% small bowel resection and anastomosis (SBS group) and were treated with intraperitoneal TGF-alpha (75 microg/kg) from the ninth postoperative day (SBS-TGF-alpha group). Parameters of intestinal adaptation (overall bowel and mucosal weight, mucosal DNA and protein, villus height, and crypt depth), enterocyte proliferation, and apoptosis were determined on day 15. Statistical significance was determined by ANOVA with a P < 0.05 considered significant. RESULTS: SBS-TGF-alpha rats demonstrated a significant increase (vs SBS) in duodenal, jejunal, and ileal overall bowel and mucosal weights; ileal mucosal DNA and protein; and jejunal and ileal villus height. SBS-TGF-alpha rats also showed an increased cell proliferation index in jejunum (704 +/- 43 vs 499 +/- 63 BrdU-positive cells/10 crypts, P < 0.05) and ileum (715 +/- 84 vs 529 +/- 40 BrdU-positive cells/10 crypts, P < 0.05) and decreased apoptotic index in ileum (8.7 +/- 1.1 vs 21.8 +/- 3.2 apoptotic cells/1,000 villus cells, P < 0.05) compared to SBS animals. CONCLUSIONS: In a rat model of SBS, TGF-alpha enhances intestinal adaptation. Possible mechanisms may include increased cell proliferation and decreased enterocyte loss via apoptosis. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/12505047/Effect_of_transforming_growth_factor_alpha_on_intestinal_adaptation_in_a_rat_model_of_short_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -