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[Extended-spectrum beta-lactamase in Klebsiella pneumoniae and Escherichia coli isolates].
Zhonghua Yi Xue Za Zhi. 2002 Nov 10; 82(21):1476-9.ZY

Abstract

OBJECTIVE

To investigate the prevalence, drug resistance, gene typing, and epidemicity of extended-spectrum beta-lactamases (ESBL) Klebsiella pneumoniae and Escherichia coli isolates.

METHODS

559 strains of K. pneumoniae and 427 strains of E.coli were isolated form Huanshan Hospital from 1 January to 31 December 1999. The ESBL-producing strains were detected by double disc test and confirmed by minimal inhibitory concentration (MIC). The MIC of ESBL-producing strains was detected by agar dilution test. The beta-lactamase genes were detected by PCR. DNA fingerprinting was made by pulsed-field gel electrophoresis (PFGE).

RESULTS

The incidence of ESBL-producing strains was 51% among the isolated K. pneumoniae (285/559) and 23.6% among the isolated E. coli (101/427), most of which were collected from the patients in the intensive care unit and neurosurgical ward. 63.5% of the ESBL-producing K. pneumoniae strains were collected from sputum specimens, and 64.3% of the ESBL-producing E. coli strains were collected from the urine specimens. Most ESBL-producing strains were resistant to most beta-lactam antibiotics, including the third-generation cephalosporins, and non- beta-lactam antimicrobial drugs, such as fluoroquinolones, aminoglycosides, tetracycline, and chloramphenicol. Most of the ESBL-producing strains were susceptible to imipenam, cefmetazole, and beta-lactam antibiotic/clavulanic acid. TEM type beta-lactamase was the main type among those EBSL-producing strains, followed by SHV type and CTX-M type. Some ESBL-producing E. coli and most ESBL-producing K. pneumoniae produced more than one type of beta-lactamase.

CONCLUSION

ESBL-producing strains are common among hospital strains of E. coli and K. pneumoniae. Most of them are multidrug resistant. Prevalence and transmission of these strains exist in hospital.

Authors+Show Affiliations

Institute of Antibiotics, Huanshan Hospital, Fudan University, Shanghai 200040, China. Xiongzzh@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12509910

Citation

Xiong, Zizhong, et al. "[Extended-spectrum Beta-lactamase in Klebsiella Pneumoniae and Escherichia Coli Isolates]." Zhonghua Yi Xue Za Zhi, vol. 82, no. 21, 2002, pp. 1476-9.
Xiong Z, Zhu D, Zhang Y, et al. [Extended-spectrum beta-lactamase in Klebsiella pneumoniae and Escherichia coli isolates]. Zhonghua Yi Xue Za Zhi. 2002;82(21):1476-9.
Xiong, Z., Zhu, D., Zhang, Y., & Wang, F. (2002). [Extended-spectrum beta-lactamase in Klebsiella pneumoniae and Escherichia coli isolates]. Zhonghua Yi Xue Za Zhi, 82(21), 1476-9.
Xiong Z, et al. [Extended-spectrum Beta-lactamase in Klebsiella Pneumoniae and Escherichia Coli Isolates]. Zhonghua Yi Xue Za Zhi. 2002 Nov 10;82(21):1476-9. PubMed PMID: 12509910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Extended-spectrum beta-lactamase in Klebsiella pneumoniae and Escherichia coli isolates]. AU - Xiong,Zizhong, AU - Zhu,Demei, AU - Zhang,Yingyuan, AU - Wang,Fu, PY - 2003/1/3/pubmed PY - 2003/7/29/medline PY - 2003/1/3/entrez SP - 1476 EP - 9 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 82 IS - 21 N2 - OBJECTIVE: To investigate the prevalence, drug resistance, gene typing, and epidemicity of extended-spectrum beta-lactamases (ESBL) Klebsiella pneumoniae and Escherichia coli isolates. METHODS: 559 strains of K. pneumoniae and 427 strains of E.coli were isolated form Huanshan Hospital from 1 January to 31 December 1999. The ESBL-producing strains were detected by double disc test and confirmed by minimal inhibitory concentration (MIC). The MIC of ESBL-producing strains was detected by agar dilution test. The beta-lactamase genes were detected by PCR. DNA fingerprinting was made by pulsed-field gel electrophoresis (PFGE). RESULTS: The incidence of ESBL-producing strains was 51% among the isolated K. pneumoniae (285/559) and 23.6% among the isolated E. coli (101/427), most of which were collected from the patients in the intensive care unit and neurosurgical ward. 63.5% of the ESBL-producing K. pneumoniae strains were collected from sputum specimens, and 64.3% of the ESBL-producing E. coli strains were collected from the urine specimens. Most ESBL-producing strains were resistant to most beta-lactam antibiotics, including the third-generation cephalosporins, and non- beta-lactam antimicrobial drugs, such as fluoroquinolones, aminoglycosides, tetracycline, and chloramphenicol. Most of the ESBL-producing strains were susceptible to imipenam, cefmetazole, and beta-lactam antibiotic/clavulanic acid. TEM type beta-lactamase was the main type among those EBSL-producing strains, followed by SHV type and CTX-M type. Some ESBL-producing E. coli and most ESBL-producing K. pneumoniae produced more than one type of beta-lactamase. CONCLUSION: ESBL-producing strains are common among hospital strains of E. coli and K. pneumoniae. Most of them are multidrug resistant. Prevalence and transmission of these strains exist in hospital. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/12509910/[Extended_spectrum_beta_lactamase_in_Klebsiella_pneumoniae_and_Escherichia_coli_isolates]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2002&vol=82&issue=21&fpage=1476 DB - PRIME DP - Unbound Medicine ER -