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In vitro antioxidant activity of some selected Prunus species in Korea.

Abstract

In the course of the investigations of natural antioxidants, we examined the antioxidant activities of the methanol (MeOH) extracts of some selected Prunus species, including P buergeriana, P davidiana, P padus, P pendula for. ascendens, P. sargentii, P. serrulata var. spontanea and P. yedoensis by three methods as represented by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, total ROS (reactive oxygen species) and the peroxynitrite (ONOO-) scavenging activity tests. We also evaluated the activities of the organic solvent-soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (n-BuOH) fractions and the water (H2O) layer of P serrulata var. spontanea leaves. By means of bioassay-directed fractionation, we isolated eleven known flavonoids (1-11) from the EtOAc soluble fraction of the MeOH extract of the Prunus serrulata var. spontanea leaves, exhibiting strong antioxidant activity and characterized as prunetin (1), genistein (2), quercetin (3), prunetin 4'-O-beta-glucopyranoside (4), kaempferol 3-O-alpha-arabinofuranoside (5), prunetin 5-O-beta-glucopyranoside (6), kaempferol 3-O-beta-xylopyranoside (7), genistin (8), kaempferol 3-O-beta-glucopyranoside (9), quercetin 3-O-beta-glucopyranoside (10) and kaempferol 3-O-beta-xylopyranosyl-(1-->2)-beta-glucopyranoside (11). Compounds 3 and 10 showed good activities in all tested model systems. Compounds 2 and 8 showed scavenging activities in the DPPH and ONOO- tests, while compounds 5, 7, 9 and 11 were active in the ONOO- and ROS tests. On the other hand, compounds 1, 4 and 6 did not show any activities in the tested model systems.

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  • Authors+Show Affiliations

    ,

    Faculty of Food Science and Biotechnology, Pukyong National University, Pusan 608-737, Korea.

    , ,

    Source

    Archives of pharmacal research 25:6 2002 Dec pg 865-72

    MeSH

    Animals
    Antioxidants
    Free Radical Scavengers
    Kidney
    Korea
    Male
    Plant Extracts
    Plant Leaves
    Plant Stems
    Prunus
    Rats
    Rats, Wistar

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    12510840

    Citation

    Jung, Hyun Ah, et al. "In Vitro Antioxidant Activity of some Selected Prunus Species in Korea." Archives of Pharmacal Research, vol. 25, no. 6, 2002, pp. 865-72.
    Jung HA, Kim AR, Chung HY, et al. In vitro antioxidant activity of some selected Prunus species in Korea. Arch Pharm Res. 2002;25(6):865-72.
    Jung, H. A., Kim, A. R., Chung, H. Y., & Choi, J. S. (2002). In vitro antioxidant activity of some selected Prunus species in Korea. Archives of Pharmacal Research, 25(6), pp. 865-72.
    Jung HA, et al. In Vitro Antioxidant Activity of some Selected Prunus Species in Korea. Arch Pharm Res. 2002;25(6):865-72. PubMed PMID: 12510840.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro antioxidant activity of some selected Prunus species in Korea. AU - Jung,Hyun Ah, AU - Kim,Ae Ra, AU - Chung,Hae Young, AU - Choi,Jae Sue, PY - 2003/1/4/pubmed PY - 2003/6/24/medline PY - 2003/1/4/entrez SP - 865 EP - 72 JF - Archives of pharmacal research JO - Arch. Pharm. Res. VL - 25 IS - 6 N2 - In the course of the investigations of natural antioxidants, we examined the antioxidant activities of the methanol (MeOH) extracts of some selected Prunus species, including P buergeriana, P davidiana, P padus, P pendula for. ascendens, P. sargentii, P. serrulata var. spontanea and P. yedoensis by three methods as represented by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, total ROS (reactive oxygen species) and the peroxynitrite (ONOO-) scavenging activity tests. We also evaluated the activities of the organic solvent-soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (n-BuOH) fractions and the water (H2O) layer of P serrulata var. spontanea leaves. By means of bioassay-directed fractionation, we isolated eleven known flavonoids (1-11) from the EtOAc soluble fraction of the MeOH extract of the Prunus serrulata var. spontanea leaves, exhibiting strong antioxidant activity and characterized as prunetin (1), genistein (2), quercetin (3), prunetin 4'-O-beta-glucopyranoside (4), kaempferol 3-O-alpha-arabinofuranoside (5), prunetin 5-O-beta-glucopyranoside (6), kaempferol 3-O-beta-xylopyranoside (7), genistin (8), kaempferol 3-O-beta-glucopyranoside (9), quercetin 3-O-beta-glucopyranoside (10) and kaempferol 3-O-beta-xylopyranosyl-(1-->2)-beta-glucopyranoside (11). Compounds 3 and 10 showed good activities in all tested model systems. Compounds 2 and 8 showed scavenging activities in the DPPH and ONOO- tests, while compounds 5, 7, 9 and 11 were active in the ONOO- and ROS tests. On the other hand, compounds 1, 4 and 6 did not show any activities in the tested model systems. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/12510840/In_vitro_antioxidant_activity_of_some_selected_Prunus_species_in_Korea_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=12510840.ui DB - PRIME DP - Unbound Medicine ER -