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The role of reactive oxygen species in action of nitric oxide-donors on stress-induced gastric mucosal lesions.
J Physiol Pharmacol. 2002 Dec; 53(4 Pt 2):761-73.JP

Abstract

The experimental model of acute gastritis such as water immersion restraint (WRS) stress-induced gastric injury is useful tool in examination of pathomechanism of acute gastritis. Nitric oxide (NO) plays an important role in the maintenance of gastric barrier, however, the interaction between reactive oxygen species (ROS) and NO on gastric mucosal integrity has been little studied. The purpose of our present study was to explain the participation of ROS in healing of WRS-induced gastric lesions accelerated by NO. Experiments were carrying out on 120 male Wistar rats. To assess gastric blood flow (GBF) laser Doppler flowmeter was used and the number of gastric lesions was counted in each stomach. The colorimetric assays were used to determine gastric tissue level of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), the products of lipid peroxidation by ROS, as well as superoxide dismutase (SOD) activity, the enzyme scavanger of ROS. We demonstrated that 3.5 h of WRS resulted in appearance of acute gastric lesions accompanied by a significant decrease of GBF. Biological effects of ROS were estimated by measuring tissue levels of MDA and 4-HNE, as well as the SOD activity. It was demonstrated that 3.5 h of WRS led to significant increase of mucosal levels of MDA and 4-HNE, and it was accompanied by a decrease of SOD activity. Pretreatment with NO-donors (SIN-1, SNAP, nitroglycerin, NO-ASA) resulted in reduction in gastric lesion number, increment of GBF, decrease of MDA and 4-HNE tissue level and increase of SOD activity. Suppression of ROS plays an important role in the action of NO-donors on healing of acute gastric lesions induced by 3.5 h of WRS. NO-donors caused an attenuation of lipid peroxidation as documented by a decrease of MDA and 4-HNE levels and enhancement of antioxidative properties as evidenced by an increase of SOD activity.

Authors+Show Affiliations

Department of Physiology, Jagiellonian University School of Medicine, Cracow, Poland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12510862

Citation

Kwiecień, S, et al. "The Role of Reactive Oxygen Species in Action of Nitric Oxide-donors On Stress-induced Gastric Mucosal Lesions." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 53, no. 4 Pt 2, 2002, pp. 761-73.
Kwiecień S, Brzozowski T, Konturek PCh, et al. The role of reactive oxygen species in action of nitric oxide-donors on stress-induced gastric mucosal lesions. J Physiol Pharmacol. 2002;53(4 Pt 2):761-73.
Kwiecień, S., Brzozowski, T., Konturek, P. C. h., & Konturek, S. J. (2002). The role of reactive oxygen species in action of nitric oxide-donors on stress-induced gastric mucosal lesions. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 53(4 Pt 2), 761-73.
Kwiecień S, et al. The Role of Reactive Oxygen Species in Action of Nitric Oxide-donors On Stress-induced Gastric Mucosal Lesions. J Physiol Pharmacol. 2002;53(4 Pt 2):761-73. PubMed PMID: 12510862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of reactive oxygen species in action of nitric oxide-donors on stress-induced gastric mucosal lesions. AU - Kwiecień,S, AU - Brzozowski,T, AU - Konturek,P Ch, AU - Konturek,S J, PY - 2003/1/4/pubmed PY - 2003/6/5/medline PY - 2003/1/4/entrez SP - 761 EP - 73 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 53 IS - 4 Pt 2 N2 - The experimental model of acute gastritis such as water immersion restraint (WRS) stress-induced gastric injury is useful tool in examination of pathomechanism of acute gastritis. Nitric oxide (NO) plays an important role in the maintenance of gastric barrier, however, the interaction between reactive oxygen species (ROS) and NO on gastric mucosal integrity has been little studied. The purpose of our present study was to explain the participation of ROS in healing of WRS-induced gastric lesions accelerated by NO. Experiments were carrying out on 120 male Wistar rats. To assess gastric blood flow (GBF) laser Doppler flowmeter was used and the number of gastric lesions was counted in each stomach. The colorimetric assays were used to determine gastric tissue level of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), the products of lipid peroxidation by ROS, as well as superoxide dismutase (SOD) activity, the enzyme scavanger of ROS. We demonstrated that 3.5 h of WRS resulted in appearance of acute gastric lesions accompanied by a significant decrease of GBF. Biological effects of ROS were estimated by measuring tissue levels of MDA and 4-HNE, as well as the SOD activity. It was demonstrated that 3.5 h of WRS led to significant increase of mucosal levels of MDA and 4-HNE, and it was accompanied by a decrease of SOD activity. Pretreatment with NO-donors (SIN-1, SNAP, nitroglycerin, NO-ASA) resulted in reduction in gastric lesion number, increment of GBF, decrease of MDA and 4-HNE tissue level and increase of SOD activity. Suppression of ROS plays an important role in the action of NO-donors on healing of acute gastric lesions induced by 3.5 h of WRS. NO-donors caused an attenuation of lipid peroxidation as documented by a decrease of MDA and 4-HNE levels and enhancement of antioxidative properties as evidenced by an increase of SOD activity. SN - 0867-5910 UR - https://www.unboundmedicine.com/medline/citation/12510862/The_role_of_reactive_oxygen_species_in_action_of_nitric_oxide_donors_on_stress_induced_gastric_mucosal_lesions_ L2 - http://www.jpp.krakow.pl/journal/archive/12_02/pdf/761_12_02_article.pdf DB - PRIME DP - Unbound Medicine ER -