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Transcriptional regulation of the human Sp1 gene promoter by the specificity protein (Sp) family members nuclear factor Y (NF-Y) and E2F.
Biochem J. 2003 Apr 15; 371(Pt 2):265-75.BJ

Abstract

We analysed in detail the minimal promoter of transcription factor Sp1, which extends 217 bp from the initiation of transcription. Within this sequence we identified putative binding sites for Sp1, nuclear factor Y (NF-Y), activator protein 2 ('AP-2'), CCAAT/enhancer-binding protein ('C/EBP') and E2F transcription factors. In one case, the boxes for Sp1 and NF-Y are overlapping. Gel-shift and supershift assays demonstrated specific binding of Sp1, Sp3 and NF-Y proteins. Transient transfections and luciferase assays revealed activation of the Sp1 minimal promoter upon overexpression of Sp1 itself, NF-Y and E2F. Whereas overexpression of NF-Y or E2F had an additive effect on Sp1 overexpression, the activation of Sp1 transcription due to Sp1 was counteracted by Sp3 overexpression. Mutagenesis analysis of the NFY/Sp1-overlapping box revealed that both factors compete for this box, and that when the NF-Y site of this overlapping box is specifically mutated there is an increase in Sp1 binding, thus increasing transcriptional activity. These results help to explain the complex regulation of the Sp1 gene, which depends on the relative amounts of Sp1, Sp3, E2F and NF-Y proteins in the cell.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, E-08028 Barcelona, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12513689

Citation

Nicolás, Marta, et al. "Transcriptional Regulation of the Human Sp1 Gene Promoter By the Specificity Protein (Sp) Family Members Nuclear Factor Y (NF-Y) and E2F." The Biochemical Journal, vol. 371, no. Pt 2, 2003, pp. 265-75.
Nicolás M, Noé V, Ciudad CJ. Transcriptional regulation of the human Sp1 gene promoter by the specificity protein (Sp) family members nuclear factor Y (NF-Y) and E2F. Biochem J. 2003;371(Pt 2):265-75.
Nicolás, M., Noé, V., & Ciudad, C. J. (2003). Transcriptional regulation of the human Sp1 gene promoter by the specificity protein (Sp) family members nuclear factor Y (NF-Y) and E2F. The Biochemical Journal, 371(Pt 2), 265-75.
Nicolás M, Noé V, Ciudad CJ. Transcriptional Regulation of the Human Sp1 Gene Promoter By the Specificity Protein (Sp) Family Members Nuclear Factor Y (NF-Y) and E2F. Biochem J. 2003 Apr 15;371(Pt 2):265-75. PubMed PMID: 12513689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional regulation of the human Sp1 gene promoter by the specificity protein (Sp) family members nuclear factor Y (NF-Y) and E2F. AU - Nicolás,Marta, AU - Noé,Vèronique, AU - Ciudad,Carlos J, PY - 2003/01/03/accepted PY - 2002/12/05/revised PY - 2002/07/25/received PY - 2003/1/7/pubmed PY - 2003/7/4/medline PY - 2003/1/7/entrez SP - 265 EP - 75 JF - The Biochemical journal JO - Biochem J VL - 371 IS - Pt 2 N2 - We analysed in detail the minimal promoter of transcription factor Sp1, which extends 217 bp from the initiation of transcription. Within this sequence we identified putative binding sites for Sp1, nuclear factor Y (NF-Y), activator protein 2 ('AP-2'), CCAAT/enhancer-binding protein ('C/EBP') and E2F transcription factors. In one case, the boxes for Sp1 and NF-Y are overlapping. Gel-shift and supershift assays demonstrated specific binding of Sp1, Sp3 and NF-Y proteins. Transient transfections and luciferase assays revealed activation of the Sp1 minimal promoter upon overexpression of Sp1 itself, NF-Y and E2F. Whereas overexpression of NF-Y or E2F had an additive effect on Sp1 overexpression, the activation of Sp1 transcription due to Sp1 was counteracted by Sp3 overexpression. Mutagenesis analysis of the NFY/Sp1-overlapping box revealed that both factors compete for this box, and that when the NF-Y site of this overlapping box is specifically mutated there is an increase in Sp1 binding, thus increasing transcriptional activity. These results help to explain the complex regulation of the Sp1 gene, which depends on the relative amounts of Sp1, Sp3, E2F and NF-Y proteins in the cell. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/12513689/Transcriptional_regulation_of_the_human_Sp1_gene_promoter_by_the_specificity_protein__Sp__family_members_nuclear_factor_Y__NF_Y__and_E2F_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BJ20021166 DB - PRIME DP - Unbound Medicine ER -