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Structure of the L1 protuberance in the ribosome.
Nat Struct Biol. 2003 Feb; 10(2):104-8.NS

Abstract

The L1 protuberance of the 50S ribosomal subunit is implicated in the release/disposal of deacylated tRNA from the E site. The apparent mobility of this ribosomal region has thus far prevented an accurate determination of its three-dimensional structure within either the 50S subunit or the 70S ribosome. Here we report the crystal structure at 2.65 A resolution of ribosomal protein L1 from Sulfolobus acidocaldarius in complex with a specific 55-nucleotide fragment of 23S rRNA from Thermus thermophilus. This structure fills a major gap in current models of the 50S ribosomal subunit. The conformations of L1 and of the rRNA fragment differ dramatically from those within the crystallographic model of the T. thermophilus 70S ribosome. Incorporation of the L1-rRNA complex into the structural models of the T. thermophilus 70S ribosome and the Deinococcus radiodurans 50S subunit gives a reliable representation of most of the L1 protuberance within the ribosome.

Authors+Show Affiliations

Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12514741

Citation

Nikulin, Alexei, et al. "Structure of the L1 Protuberance in the Ribosome." Nature Structural Biology, vol. 10, no. 2, 2003, pp. 104-8.
Nikulin A, Eliseikina I, Tishchenko S, et al. Structure of the L1 protuberance in the ribosome. Nat Struct Biol. 2003;10(2):104-8.
Nikulin, A., Eliseikina, I., Tishchenko, S., Nevskaya, N., Davydova, N., Platonova, O., Piendl, W., Selmer, M., Liljas, A., Drygin, D., Zimmermann, R., Garber, M., & Nikonov, S. (2003). Structure of the L1 protuberance in the ribosome. Nature Structural Biology, 10(2), 104-8.
Nikulin A, et al. Structure of the L1 Protuberance in the Ribosome. Nat Struct Biol. 2003;10(2):104-8. PubMed PMID: 12514741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure of the L1 protuberance in the ribosome. AU - Nikulin,Alexei, AU - Eliseikina,Irina, AU - Tishchenko,Svetlana, AU - Nevskaya,Natalia, AU - Davydova,Natalia, AU - Platonova,Olga, AU - Piendl,Wolfgang, AU - Selmer,Maria, AU - Liljas,Anders, AU - Drygin,Denis, AU - Zimmermann,Robert, AU - Garber,Maria, AU - Nikonov,Stanislav, PY - 2002/10/23/received PY - 2002/11/27/accepted PY - 2003/1/7/pubmed PY - 2003/2/26/medline PY - 2003/1/7/entrez SP - 104 EP - 8 JF - Nature structural biology JO - Nat Struct Biol VL - 10 IS - 2 N2 - The L1 protuberance of the 50S ribosomal subunit is implicated in the release/disposal of deacylated tRNA from the E site. The apparent mobility of this ribosomal region has thus far prevented an accurate determination of its three-dimensional structure within either the 50S subunit or the 70S ribosome. Here we report the crystal structure at 2.65 A resolution of ribosomal protein L1 from Sulfolobus acidocaldarius in complex with a specific 55-nucleotide fragment of 23S rRNA from Thermus thermophilus. This structure fills a major gap in current models of the 50S ribosomal subunit. The conformations of L1 and of the rRNA fragment differ dramatically from those within the crystallographic model of the T. thermophilus 70S ribosome. Incorporation of the L1-rRNA complex into the structural models of the T. thermophilus 70S ribosome and the Deinococcus radiodurans 50S subunit gives a reliable representation of most of the L1 protuberance within the ribosome. SN - 1072-8368 UR - https://www.unboundmedicine.com/medline/citation/12514741/Structure_of_the_L1_protuberance_in_the_ribosome_ DB - PRIME DP - Unbound Medicine ER -