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[Effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve].

Abstract

OBJECTIVE

To investigate the effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve.

METHODS

Sciatic nerve injury model was established by transection of right side sciatic nerve in 90 male SD rats, which were randomly divided into two groups. The experimental group was treated with electroacupuncture, no treatment in the control group. The distal part of the injured nerve was harvested after 1, 2, 4, 6 and 10 weeks of operation and stored in the liquid nitrogen. The total RNA was extracted by the TRIzol reagent. Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detected the mRNA expression of NGF and IGF-1.

RESULTS

The mRNA expression of NGF in the experimental group was increased quickly from the second week, and reached to highest level in the fourth week. It was much higher than that of the control group (P < 0.05). Then it began to decline in following time and approximately reached to the level of the first week after 10 weeks of operation. The mRNA expression of IGF-1 in the experimental group was remarkably increased in the second and fourth week, and which was much higher than that of the control group respectively(P < 0.05). Although the mRNA expression of IGF-1 after 10 weeks of operation in the experimental group was higher than that of the control group, but there was no significant difference between the two groups(P > 0.05). There was linear correlation in the fourth week between mRNA expression of NGF and IGF-1 in the experimental group.

CONCLUSION

The mRNA expression of NGF and IGF-1 can be elevated in injured nerve at early stage interfered with electroacupuncture.

Authors+Show Affiliations

Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200092.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12516429

Citation

Chen, X D., et al. "[Effect of Electroacupuncture On mRNA Expression of NGF and IGF-1 in Injured Nerve]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 14, no. 6, 2000, pp. 328-31.
Chen XD, Gu YD, Yang Y. [Effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2000;14(6):328-31.
Chen, X. D., Gu, Y. D., & Yang, Y. (2000). [Effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 14(6), pp. 328-31.
Chen XD, Gu YD, Yang Y. [Effect of Electroacupuncture On mRNA Expression of NGF and IGF-1 in Injured Nerve]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2000;14(6):328-31. PubMed PMID: 12516429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve]. AU - Chen,X D, AU - Gu,Y D, AU - Yang,Y, PY - 2003/1/9/pubmed PY - 2003/2/7/medline PY - 2003/1/9/entrez SP - 328 EP - 31 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 14 IS - 6 N2 - OBJECTIVE: To investigate the effect of electroacupuncture on mRNA expression of NGF and IGF-1 in injured nerve. METHODS: Sciatic nerve injury model was established by transection of right side sciatic nerve in 90 male SD rats, which were randomly divided into two groups. The experimental group was treated with electroacupuncture, no treatment in the control group. The distal part of the injured nerve was harvested after 1, 2, 4, 6 and 10 weeks of operation and stored in the liquid nitrogen. The total RNA was extracted by the TRIzol reagent. Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detected the mRNA expression of NGF and IGF-1. RESULTS: The mRNA expression of NGF in the experimental group was increased quickly from the second week, and reached to highest level in the fourth week. It was much higher than that of the control group (P < 0.05). Then it began to decline in following time and approximately reached to the level of the first week after 10 weeks of operation. The mRNA expression of IGF-1 in the experimental group was remarkably increased in the second and fourth week, and which was much higher than that of the control group respectively(P < 0.05). Although the mRNA expression of IGF-1 after 10 weeks of operation in the experimental group was higher than that of the control group, but there was no significant difference between the two groups(P > 0.05). There was linear correlation in the fourth week between mRNA expression of NGF and IGF-1 in the experimental group. CONCLUSION: The mRNA expression of NGF and IGF-1 can be elevated in injured nerve at early stage interfered with electroacupuncture. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/12516429/[Effect_of_electroacupuncture_on_mRNA_expression_of_NGF_and_IGF_1_in_injured_nerve]_ DB - PRIME DP - Unbound Medicine ER -