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Calcium exerts a larger regulatory effect on potassium+ channels in small mesenteric artery mycoytes from spontaneously hypertensive rats compared to Wistar-Kyoto rats.
Am J Hypertens. 2003 Jan; 16(1):21-7.AJ

Abstract

Hypertension is associated with a remodeling of arterial smooth muscle K(+) channels with Ca(2+)-gated K(+) channel (BK(Ca)) activity being enhanced and voltage-gated K(+) channel (K(v)) activity depressed. Because both of these channel types are modulated by intracellular Ca(2+), we tested the hypothesis that Ca(2+) had a larger effect on both BK(Ca) and K(v) channels in arterial myocytes from hypertensive animals. Myocytes were enzymatically dispersed from small mesenteric arteries (SMA) of 12-week-old Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). Using whole cell patch clamp methods, BK(Ca) and K(v) current components were determined as iberiotoxin-sensitive and -insensitive currents, respectively. The effects of Ca(2+) on these K(+) current components were determined from measurements made with 0.2 and 2 mmol/L external Ca(2+). Increasing external Ca(2+) from 0.2 to 2 mmol/L Ca(2+) increased BK(Ca) currents recorded using myocytes from both WKY rats and SHR with a larger effect in SHR. Increasing external Ca(2+) decreased K(v) currents recorded using myocytes from both WKY and SHR also with a larger effect in SHR. In other experiments, currents through voltage-gated Ca(2+) channels (Ca(v)) measured at 0.2 mmol/L external Ca(2+) were 12 +/- 2% (n = 12) of those recorded at 2 mmol/L Ca(2+) with no differences in percent effect between WKY and SHR. In isolated SMA segments, isometric force development in response to 140 mmol/L KCl at 0.2 mmol/L external Ca(2+) was about 23 +/- 6% (n = 8) of that measured at 2 mmol/L external Ca(2+). These results suggest that an increase in Ca(2+) influx through Ca(v) or in intracellular Ca(2+) secondary to an increase in external Ca(2+) augments BK(Ca) currents and inhibits K(v) currents in SMA myocytes with a larger effect in SHR compared to WKY. This mechanism may contribute to the functional remodeling of K(+) currents of arterial myocytes in hypertensive animals.

Authors+Show Affiliations

Cox RH
Lankenau Institute for Medical Research, Jefferson Health System, Philadelphia, Pennsylvania, USA. coxr@mlhs.org
Lozinskaya I
No affiliation info available
Dietz NJ
No affiliation info available

MeSH

AnimalsCalciumCalcium ChannelsHypertensionLarge-Conductance Calcium-Activated Potassium ChannelsMembrane PotentialsMesenteric ArteriesMyocytes, Smooth MusclePatch-Clamp TechniquesPotassiumPotassium Channels, Calcium-ActivatedPotassium Channels, Voltage-GatedRatsRats, Inbred SHRRats, Inbred WKYSpecies Specificity

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12517678

Citation

Cox, Robert H., et al. "Calcium Exerts a Larger Regulatory Effect On Potassium+ Channels in Small Mesenteric Artery Mycoytes From Spontaneously Hypertensive Rats Compared to Wistar-Kyoto Rats." American Journal of Hypertension, vol. 16, no. 1, 2003, pp. 21-7.
Cox RH, Lozinskaya I, Dietz NJ. Calcium exerts a larger regulatory effect on potassium+ channels in small mesenteric artery mycoytes from spontaneously hypertensive rats compared to Wistar-Kyoto rats. Am J Hypertens. 2003;16(1):21-7.
Cox, R. H., Lozinskaya, I., & Dietz, N. J. (2003). Calcium exerts a larger regulatory effect on potassium+ channels in small mesenteric artery mycoytes from spontaneously hypertensive rats compared to Wistar-Kyoto rats. American Journal of Hypertension, 16(1), 21-7.
Cox RH, Lozinskaya I, Dietz NJ. Calcium Exerts a Larger Regulatory Effect On Potassium+ Channels in Small Mesenteric Artery Mycoytes From Spontaneously Hypertensive Rats Compared to Wistar-Kyoto Rats. Am J Hypertens. 2003;16(1):21-7. PubMed PMID: 12517678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calcium exerts a larger regulatory effect on potassium+ channels in small mesenteric artery mycoytes from spontaneously hypertensive rats compared to Wistar-Kyoto rats. AU - Cox,Robert H, AU - Lozinskaya,Irina, AU - Dietz,Nancy J, PY - 2003/1/9/pubmed PY - 2003/7/17/medline PY - 2003/1/9/entrez SP - 21 EP - 7 JF - American journal of hypertension JO - Am J Hypertens VL - 16 IS - 1 N2 - Hypertension is associated with a remodeling of arterial smooth muscle K(+) channels with Ca(2+)-gated K(+) channel (BK(Ca)) activity being enhanced and voltage-gated K(+) channel (K(v)) activity depressed. Because both of these channel types are modulated by intracellular Ca(2+), we tested the hypothesis that Ca(2+) had a larger effect on both BK(Ca) and K(v) channels in arterial myocytes from hypertensive animals. Myocytes were enzymatically dispersed from small mesenteric arteries (SMA) of 12-week-old Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). Using whole cell patch clamp methods, BK(Ca) and K(v) current components were determined as iberiotoxin-sensitive and -insensitive currents, respectively. The effects of Ca(2+) on these K(+) current components were determined from measurements made with 0.2 and 2 mmol/L external Ca(2+). Increasing external Ca(2+) from 0.2 to 2 mmol/L Ca(2+) increased BK(Ca) currents recorded using myocytes from both WKY rats and SHR with a larger effect in SHR. Increasing external Ca(2+) decreased K(v) currents recorded using myocytes from both WKY and SHR also with a larger effect in SHR. In other experiments, currents through voltage-gated Ca(2+) channels (Ca(v)) measured at 0.2 mmol/L external Ca(2+) were 12 +/- 2% (n = 12) of those recorded at 2 mmol/L Ca(2+) with no differences in percent effect between WKY and SHR. In isolated SMA segments, isometric force development in response to 140 mmol/L KCl at 0.2 mmol/L external Ca(2+) was about 23 +/- 6% (n = 8) of that measured at 2 mmol/L external Ca(2+). These results suggest that an increase in Ca(2+) influx through Ca(v) or in intracellular Ca(2+) secondary to an increase in external Ca(2+) augments BK(Ca) currents and inhibits K(v) currents in SMA myocytes with a larger effect in SHR compared to WKY. This mechanism may contribute to the functional remodeling of K(+) currents of arterial myocytes in hypertensive animals. SN - 0895-7061 UR - https://www.unboundmedicine.com/medline/citation/12517678/Calcium_exerts_a_larger_regulatory_effect_on_potassium+_channels_in_small_mesenteric_artery_mycoytes_from_spontaneously_hypertensive_rats_compared_to_Wistar_Kyoto_rats_ DB - PRIME DP - Unbound Medicine ER -