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Effect of fish oil on LDL oxidation and plasma homocysteine concentrations in health.
J Lab Clin Med. 2003 Jan; 141(1):41-9.JL

Abstract

Oxidation of low-density lipoprotein (LDL) and hyperhomocysteinemia are believed to play a role in therogenesis. Whether n-3 polyunsaturated fatty acids increase LDL susceptibility to oxidation or influence homocysteine (Hcy) metabolism has long been a subject of controversy. In this study, we evaluated the effect of 8 weeks of dietary supplementation with 6 g/day of fish oil (FO; 3 g of n-3 fatty acids) on plasma lipoproteins, in vitro LDL peroxidation, antioxidant status, and plasma Hcy concentrations in 16 normolipidemic subjects. FO rapidly and significantly (P < .01) decreased plasma total and very low density lipoprotein triglyceride concentrations and had no effect on LDL or high-density-lipoprotein cholesterol. The mean lag time before onset of Cu(2+)-induced LDL oxidation, as well as plasma and LDL alpha-tocopherol and beta-carotene concentrations, was unchanged. However, changes in plasma aminothiol concentrations occurred during the study. Specifically, a progressive and significant increase in total Hcy plasma concentrations was observed (13.4% and 20% after 4 and 8 weeks, respectively; P < .01). Total glutathione concentrations were significantly higher after 8 weeks (P < .05). The tHcy increase was not associated with changes in plasma folate or vitamin B(12) concentrations. However, concentrations of plasma nitric oxide metabolites (NO(x) = NO(2) + NO(3)) were significantly higher than at baseline after 8 weeks of FO intake (74%; P < .01). Further, the changes in total Hcy and NO(x) plasma concentrations observed after 8 weeks of FO were found to be significantly correlated (r = .78, P < .001). With this study, we report for the first time the apparent interaction of n-3 fatty acids and nitric oxide on Hcy metabolism.

Authors+Show Affiliations

Hyperlipidemia and Atherosclerosis Research Group of the Clinical Research Institute of Montreal and INSERM-U498-Faculté de Médecine, Université de Bourgogne, Dijon, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12518167

Citation

Piolot, Alain, et al. "Effect of Fish Oil On LDL Oxidation and Plasma Homocysteine Concentrations in Health." The Journal of Laboratory and Clinical Medicine, vol. 141, no. 1, 2003, pp. 41-9.
Piolot A, Blache D, Boulet L, et al. Effect of fish oil on LDL oxidation and plasma homocysteine concentrations in health. J Lab Clin Med. 2003;141(1):41-9.
Piolot, A., Blache, D., Boulet, L., Fortin, L. J., Dubreuil, D., Marcoux, C., Davignon, J., & Lussier-Cacan, S. (2003). Effect of fish oil on LDL oxidation and plasma homocysteine concentrations in health. The Journal of Laboratory and Clinical Medicine, 141(1), 41-9.
Piolot A, et al. Effect of Fish Oil On LDL Oxidation and Plasma Homocysteine Concentrations in Health. J Lab Clin Med. 2003;141(1):41-9. PubMed PMID: 12518167.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of fish oil on LDL oxidation and plasma homocysteine concentrations in health. AU - Piolot,Alain, AU - Blache,Denis, AU - Boulet,Lucie, AU - Fortin,Louis Jacques, AU - Dubreuil,Denise, AU - Marcoux,Caroline, AU - Davignon,Jean, AU - Lussier-Cacan,Suzanne, PY - 2003/1/9/pubmed PY - 2003/1/25/medline PY - 2003/1/9/entrez SP - 41 EP - 9 JF - The Journal of laboratory and clinical medicine JO - J Lab Clin Med VL - 141 IS - 1 N2 - Oxidation of low-density lipoprotein (LDL) and hyperhomocysteinemia are believed to play a role in therogenesis. Whether n-3 polyunsaturated fatty acids increase LDL susceptibility to oxidation or influence homocysteine (Hcy) metabolism has long been a subject of controversy. In this study, we evaluated the effect of 8 weeks of dietary supplementation with 6 g/day of fish oil (FO; 3 g of n-3 fatty acids) on plasma lipoproteins, in vitro LDL peroxidation, antioxidant status, and plasma Hcy concentrations in 16 normolipidemic subjects. FO rapidly and significantly (P < .01) decreased plasma total and very low density lipoprotein triglyceride concentrations and had no effect on LDL or high-density-lipoprotein cholesterol. The mean lag time before onset of Cu(2+)-induced LDL oxidation, as well as plasma and LDL alpha-tocopherol and beta-carotene concentrations, was unchanged. However, changes in plasma aminothiol concentrations occurred during the study. Specifically, a progressive and significant increase in total Hcy plasma concentrations was observed (13.4% and 20% after 4 and 8 weeks, respectively; P < .01). Total glutathione concentrations were significantly higher after 8 weeks (P < .05). The tHcy increase was not associated with changes in plasma folate or vitamin B(12) concentrations. However, concentrations of plasma nitric oxide metabolites (NO(x) = NO(2) + NO(3)) were significantly higher than at baseline after 8 weeks of FO intake (74%; P < .01). Further, the changes in total Hcy and NO(x) plasma concentrations observed after 8 weeks of FO were found to be significantly correlated (r = .78, P < .001). With this study, we report for the first time the apparent interaction of n-3 fatty acids and nitric oxide on Hcy metabolism. SN - 0022-2143 UR - https://www.unboundmedicine.com/medline/citation/12518167/Effect_of_fish_oil_on_LDL_oxidation_and_plasma_homocysteine_concentrations_in_health_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022214302930167 DB - PRIME DP - Unbound Medicine ER -