Tags

Type your tag names separated by a space and hit enter

Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery.
Nat Genet. 2003 Feb; 33(2):155-61.NGen

Abstract

Nuclear export of mRNA is mediated by a complex machinery of RNA-binding proteins that recognizes and routes mRNAs through a messenger ribonucleoprotein (mRNP) network. The full spectrum of mRNA cargoes for any dedicated mRNA export factor is unknown. We identified the mRNAs that bind two conserved yeast mRNA export factors, Yra1 (refs. 1-5) and Mex67 (refs. 6,7), on a genome-wide scale and determined their level of binding. Yra1 and Mex67 bind approximately 1,000 and 1,150 mRNAs, respectively, corresponding to almost 20% of the yeast genome and roughly 36% of all transcriptional events each. The binding level of Yra1 targets is related to their transcriptional frequency, but that of Mex67 targets is not. Yra1-bound transcripts are enriched in mRNAs that are regulated by a number of transcription factors. Yra1- and Mex67-bound populations also show enrichment of mRNAs encoding distinct functional classes of proteins, some of which are regulated by these transcription factors. We determined that one such transcription factor, Abf1 (refs. 8-10), associates with Yra1. These results indicate a previously unidentified specificity of mRNA export factors, which coordinates the export of transcriptionally co-regulated, functional classes of transcripts, perhaps through interactions with the transcriptional machinery.

Authors+Show Affiliations

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12524544

Citation

Hieronymus, Haley, and Pamela A. Silver. "Genome-wide Analysis of RNA-protein Interactions Illustrates Specificity of the mRNA Export Machinery." Nature Genetics, vol. 33, no. 2, 2003, pp. 155-61.
Hieronymus H, Silver PA. Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery. Nat Genet. 2003;33(2):155-61.
Hieronymus, H., & Silver, P. A. (2003). Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery. Nature Genetics, 33(2), 155-61.
Hieronymus H, Silver PA. Genome-wide Analysis of RNA-protein Interactions Illustrates Specificity of the mRNA Export Machinery. Nat Genet. 2003;33(2):155-61. PubMed PMID: 12524544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery. AU - Hieronymus,Haley, AU - Silver,Pamela A, Y1 - 2003/01/13/ PY - 2002/08/02/received PY - 2002/12/05/accepted PY - 2003/1/14/pubmed PY - 2003/3/5/medline PY - 2003/1/14/entrez SP - 155 EP - 61 JF - Nature genetics JO - Nat Genet VL - 33 IS - 2 N2 - Nuclear export of mRNA is mediated by a complex machinery of RNA-binding proteins that recognizes and routes mRNAs through a messenger ribonucleoprotein (mRNP) network. The full spectrum of mRNA cargoes for any dedicated mRNA export factor is unknown. We identified the mRNAs that bind two conserved yeast mRNA export factors, Yra1 (refs. 1-5) and Mex67 (refs. 6,7), on a genome-wide scale and determined their level of binding. Yra1 and Mex67 bind approximately 1,000 and 1,150 mRNAs, respectively, corresponding to almost 20% of the yeast genome and roughly 36% of all transcriptional events each. The binding level of Yra1 targets is related to their transcriptional frequency, but that of Mex67 targets is not. Yra1-bound transcripts are enriched in mRNAs that are regulated by a number of transcription factors. Yra1- and Mex67-bound populations also show enrichment of mRNAs encoding distinct functional classes of proteins, some of which are regulated by these transcription factors. We determined that one such transcription factor, Abf1 (refs. 8-10), associates with Yra1. These results indicate a previously unidentified specificity of mRNA export factors, which coordinates the export of transcriptionally co-regulated, functional classes of transcripts, perhaps through interactions with the transcriptional machinery. SN - 1061-4036 UR - https://www.unboundmedicine.com/medline/citation/12524544/Genome_wide_analysis_of_RNA_protein_interactions_illustrates_specificity_of_the_mRNA_export_machinery_ L2 - https://facultyopinions.com/pubmed/12524544 DB - PRIME DP - Unbound Medicine ER -