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Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis.
Nucleic Acids Res. 2003 Jan 15; 31(2):690-9.NA

Abstract

ENP1 is an essential Saccharomyces cerevisiae gene encoding a 483 amino acid polypeptide. Enp1 protein is localized in the nucleus and concentrated in the nucleolus. An enp1-1 temperature-sensitive mutant inhibited 35S pre-rRNA early processing at sites A(0), A(1) and A(2) as shown by northern analysis of steady state levels of rRNA precursors. Pulse-chase analysis further revealed that the enp1-1 strain was defective in the synthesis of 20S pre-rRNA and hence 18S rRNA, which led to reduced formation of 40S ribosomal subunits. Co-precipitation analysis revealed that Enp1 was associated with Nop1 protein, as well as with U3 and U14 RNAs, two snoRNAs implicated in early pre-rRNA processing steps. These results suggest a direct role for Enp1 in the early steps of rRNA processing.

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ncbi.nlm.nih.gov
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Authors+Show Affiliations

Chen W
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA.
Bucaria J
No affiliation info available
Band DA
No affiliation info available
Sutton A
No affiliation info available
Sternglanz R
No affiliation info available

MeSH

AllelesAmino Acid SequenceBinding SitesCell NucleolusGreen Fluorescent ProteinsLuminescent ProteinsMolecular Sequence DataMutationNuclear ProteinsProtein BindingRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Small NucleolarRecombinant Fusion ProteinsRibonucleoproteins, Small NucleolarRibosomesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidTemperature

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12527778

Citation

Chen, Weidong, et al. "Enp1, a Yeast Protein Associated With U3 and U14 snoRNAs, Is Required for pre-rRNA Processing and 40S Subunit Synthesis." Nucleic Acids Research, vol. 31, no. 2, 2003, pp. 690-9.
Chen W, Bucaria J, Band DA, et al. Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis. Nucleic Acids Res. 2003;31(2):690-9.
Chen, W., Bucaria, J., Band, D. A., Sutton, A., & Sternglanz, R. (2003). Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis. Nucleic Acids Research, 31(2), 690-9.
Chen W, et al. Enp1, a Yeast Protein Associated With U3 and U14 snoRNAs, Is Required for pre-rRNA Processing and 40S Subunit Synthesis. Nucleic Acids Res. 2003 Jan 15;31(2):690-9. PubMed PMID: 12527778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis. AU - Chen,Weidong, AU - Bucaria,Jean, AU - Band,David A, AU - Sutton,Ann, AU - Sternglanz,Rolf, PY - 2003/1/16/pubmed PY - 2003/3/7/medline PY - 2003/1/16/entrez SP - 690 EP - 9 JF - Nucleic acids research JO - Nucleic Acids Res VL - 31 IS - 2 N2 - ENP1 is an essential Saccharomyces cerevisiae gene encoding a 483 amino acid polypeptide. Enp1 protein is localized in the nucleus and concentrated in the nucleolus. An enp1-1 temperature-sensitive mutant inhibited 35S pre-rRNA early processing at sites A(0), A(1) and A(2) as shown by northern analysis of steady state levels of rRNA precursors. Pulse-chase analysis further revealed that the enp1-1 strain was defective in the synthesis of 20S pre-rRNA and hence 18S rRNA, which led to reduced formation of 40S ribosomal subunits. Co-precipitation analysis revealed that Enp1 was associated with Nop1 protein, as well as with U3 and U14 RNAs, two snoRNAs implicated in early pre-rRNA processing steps. These results suggest a direct role for Enp1 in the early steps of rRNA processing. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/12527778/Enp1_a_yeast_protein_associated_with_U3_and_U14_snoRNAs_is_required_for_pre_rRNA_processing_and_40S_subunit_synthesis_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkg145 DB - PRIME DP - Unbound Medicine ER -