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Concomitant allorecognition of mismatched donor HLA class I- and class II-derived peptides in pediatric lung transplant recipients with bronchiolitis obliterans syndrome.
J Heart Lung Transplant. 2003 Jan; 22(1):35-43.JH

Abstract

BACKGROUND

The authors' previous studies with 2 different adult patient populations demonstrated a correlation between indirect allorecognition of mismatched donor HLA Class I- and Class II-derived peptides and the development of bronchiolitis obliterans syndrome (BOS) after lung transplantation. The aim of this study was to determine whether a parallel allorecognition of mismatched donor HLA Class I- and Class II-derived peptides occurs after lung transplantation and to determine its correlation with the development of BOS after lung transplantation in a group of pediatric patients.

METHODS

Peripheral blood mononuclear cells from 7 BOS-positive and 6 BOS-negative pediatric lung transplant recipients (age, 11.5 +/- 4.4 years) were cultured in the presence of synthetic peptides corresponding to the alpha-chain hypervariable regions of a mismatched donor HLA Class I molecule and the beta-chain hypervariable region of a mismatched donor HLA-DR molecule. The frequencies of HLA Class I and Class II alloreactive T cells were determined using limiting dilution analysis.

RESULTS

A significant increase (p = 0.025) in HLA Class I-alloreactive T cells was observed in BOS-positive patients (7.1 x 10(-5) +/- 4.3 x 10(-5)) compared with BOS-negative patients (2.1 x 10(-5) +/- 1.8 x 10(-6)). In addition, a significant increase (p = 0.033) in HLA Class II-alloreactive T cells also was observed in BOS-positive patients (9.6 x 10(-5) +/- 7.9 x 10(-5)) compared with BOS-negative patients (1.3 x 10(-5) +/- 2.1 x 10(-6)).

CONCLUSIONS

This study indicates that a parallel CD4+ T-cell alloreactivity to both donor HLA Class I and Class II molecules may play a role in the pathogenesis of BOS both in adult and pediatric lung transplant recipients.

Authors+Show Affiliations

Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12531411

Citation

Lu, Kim C., et al. "Concomitant Allorecognition of Mismatched Donor HLA Class I- and Class II-derived Peptides in Pediatric Lung Transplant Recipients With Bronchiolitis Obliterans Syndrome." The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation, vol. 22, no. 1, 2003, pp. 35-43.
Lu KC, Jaramillo A, Mendeloff EN, et al. Concomitant allorecognition of mismatched donor HLA class I- and class II-derived peptides in pediatric lung transplant recipients with bronchiolitis obliterans syndrome. J Heart Lung Transplant. 2003;22(1):35-43.
Lu, K. C., Jaramillo, A., Mendeloff, E. N., Huddleston, C. B., Sweet, S. C., Patterson, G. A., & Mohanakumar, T. (2003). Concomitant allorecognition of mismatched donor HLA class I- and class II-derived peptides in pediatric lung transplant recipients with bronchiolitis obliterans syndrome. The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation, 22(1), 35-43.
Lu KC, et al. Concomitant Allorecognition of Mismatched Donor HLA Class I- and Class II-derived Peptides in Pediatric Lung Transplant Recipients With Bronchiolitis Obliterans Syndrome. J Heart Lung Transplant. 2003;22(1):35-43. PubMed PMID: 12531411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Concomitant allorecognition of mismatched donor HLA class I- and class II-derived peptides in pediatric lung transplant recipients with bronchiolitis obliterans syndrome. AU - Lu,Kim C, AU - Jaramillo,Andrés, AU - Mendeloff,Eric N, AU - Huddleston,Charles B, AU - Sweet,Stuart C, AU - Patterson,G Alexander, AU - Mohanakumar,T, PY - 2003/1/18/pubmed PY - 2003/5/31/medline PY - 2003/1/18/entrez SP - 35 EP - 43 JF - The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation JO - J. Heart Lung Transplant. VL - 22 IS - 1 N2 - BACKGROUND: The authors' previous studies with 2 different adult patient populations demonstrated a correlation between indirect allorecognition of mismatched donor HLA Class I- and Class II-derived peptides and the development of bronchiolitis obliterans syndrome (BOS) after lung transplantation. The aim of this study was to determine whether a parallel allorecognition of mismatched donor HLA Class I- and Class II-derived peptides occurs after lung transplantation and to determine its correlation with the development of BOS after lung transplantation in a group of pediatric patients. METHODS: Peripheral blood mononuclear cells from 7 BOS-positive and 6 BOS-negative pediatric lung transplant recipients (age, 11.5 +/- 4.4 years) were cultured in the presence of synthetic peptides corresponding to the alpha-chain hypervariable regions of a mismatched donor HLA Class I molecule and the beta-chain hypervariable region of a mismatched donor HLA-DR molecule. The frequencies of HLA Class I and Class II alloreactive T cells were determined using limiting dilution analysis. RESULTS: A significant increase (p = 0.025) in HLA Class I-alloreactive T cells was observed in BOS-positive patients (7.1 x 10(-5) +/- 4.3 x 10(-5)) compared with BOS-negative patients (2.1 x 10(-5) +/- 1.8 x 10(-6)). In addition, a significant increase (p = 0.033) in HLA Class II-alloreactive T cells also was observed in BOS-positive patients (9.6 x 10(-5) +/- 7.9 x 10(-5)) compared with BOS-negative patients (1.3 x 10(-5) +/- 2.1 x 10(-6)). CONCLUSIONS: This study indicates that a parallel CD4+ T-cell alloreactivity to both donor HLA Class I and Class II molecules may play a role in the pathogenesis of BOS both in adult and pediatric lung transplant recipients. SN - 1053-2498 UR - https://www.unboundmedicine.com/medline/citation/12531411/Concomitant_allorecognition_of_mismatched_donor_HLA_class_I__and_class_II_derived_peptides_in_pediatric_lung_transplant_recipients_with_bronchiolitis_obliterans_syndrome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1053249802004783 DB - PRIME DP - Unbound Medicine ER -