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Differential effect of omega3 PUFA supplementations on Na,K-ATPase and Mg-ATPase activities: possible role of the membrane omega6/omega3 ratio.
J Membr Biol. 2003 Jan 01; 191(1):37-47.JM

Abstract

Several functional properties of Na,K-ATPase are strongly dependent on membrane fatty acid composition, but the underlying mechanism is still not well defined. We have studied the effects of two types of supplementations enriched in the w3 polyunsaturated fatty acids on the Na,K-ATPase and Mg-ATPase activities in sciatic nerve (SN) and red blood cells (RBC). Eight groups of rats, controls and diabetics, received a standard diet, supplemented or not with 30 or 60 mg/kg/day of docosahexaenoic acid (DHA) or with soybean for eight weeks. Diabetes induced significant decrease of Na,K-ATPase activity in SN (-23%) and RBC (-25%), without affecting Mg-ATPase activity. In RBC, soybean and DHA supplementations caused significant increases in Na,K-ATPase activity (in various range, +13% to +145%) in all groups, and in Mg-ATPase activity in control soybean (+65%), control and diabetic DHA high dose (+39%, +53%) and diabetic DHA low dose (+131%) groups. In SN, the soybean caused a significant decrease in Na,K-ATPase activity (-26%) and still more in the diabetic group (-53%). The DHA diet induced a slight decrease in activity in control groups, whilst during diabetes, at high dose, we noted an aggravation of this decrease (-36%). Mg-ATPase activity was not modified by supplementations except for the low dose of DHA where the activity was slightly decreased in the control group (-16%). The supplementations induced multiple tissue-specific modifications in the membrane fatty acid composition of RBC and of SN homogenates. Several specific correlations have been found between variations in fatty acids amounts and Na,K-ATPase activity in these tissues but only in RBC for Mg-ATPase activity. Indeed, we observed that the variations in Na,K-ATPase activity are positively and significantly correlated with changes in the omega6/omega3 ratio in SN as well as in RBC. These data clearly show, for the first time, that the diet could modulate the Na,K-ATPase activity via the omega6/omega3 ratio in the membranes. A similar correlation was observed with Mg-ATPase activity in RBC, suggesting also a dietary regulation of the enzyme; but for the SN, this activity might be regulated by a different omega6/omega3 ratio or by another pathway.

Authors+Show Affiliations

Laboratoire de Diabétologie, UPRES EA 2193, Faculté de Médecine Timone, 27 Boulevard Jean Moulin, 13385 Marseille cedex 05, France. anissa.djemli@sympatico.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12532275

Citation

Djemli-Shipkolye, A, et al. "Differential Effect of Omega3 PUFA Supplementations On Na,K-ATPase and Mg-ATPase Activities: Possible Role of the Membrane Omega6/omega3 Ratio." The Journal of Membrane Biology, vol. 191, no. 1, 2003, pp. 37-47.
Djemli-Shipkolye A, Raccah D, Pieroni G, et al. Differential effect of omega3 PUFA supplementations on Na,K-ATPase and Mg-ATPase activities: possible role of the membrane omega6/omega3 ratio. J Membr Biol. 2003;191(1):37-47.
Djemli-Shipkolye, A., Raccah, D., Pieroni, G., Vague, P., Coste, T. C., & Gerbi, A. (2003). Differential effect of omega3 PUFA supplementations on Na,K-ATPase and Mg-ATPase activities: possible role of the membrane omega6/omega3 ratio. The Journal of Membrane Biology, 191(1), 37-47.
Djemli-Shipkolye A, et al. Differential Effect of Omega3 PUFA Supplementations On Na,K-ATPase and Mg-ATPase Activities: Possible Role of the Membrane Omega6/omega3 Ratio. J Membr Biol. 2003 Jan 1;191(1):37-47. PubMed PMID: 12532275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effect of omega3 PUFA supplementations on Na,K-ATPase and Mg-ATPase activities: possible role of the membrane omega6/omega3 ratio. AU - Djemli-Shipkolye,A, AU - Raccah,D, AU - Pieroni,G, AU - Vague,P, AU - Coste,T C, AU - Gerbi,A, PY - 2001/12/03/received PY - 2003/1/18/pubmed PY - 2003/10/1/medline PY - 2003/1/18/entrez SP - 37 EP - 47 JF - The Journal of membrane biology JO - J Membr Biol VL - 191 IS - 1 N2 - Several functional properties of Na,K-ATPase are strongly dependent on membrane fatty acid composition, but the underlying mechanism is still not well defined. We have studied the effects of two types of supplementations enriched in the w3 polyunsaturated fatty acids on the Na,K-ATPase and Mg-ATPase activities in sciatic nerve (SN) and red blood cells (RBC). Eight groups of rats, controls and diabetics, received a standard diet, supplemented or not with 30 or 60 mg/kg/day of docosahexaenoic acid (DHA) or with soybean for eight weeks. Diabetes induced significant decrease of Na,K-ATPase activity in SN (-23%) and RBC (-25%), without affecting Mg-ATPase activity. In RBC, soybean and DHA supplementations caused significant increases in Na,K-ATPase activity (in various range, +13% to +145%) in all groups, and in Mg-ATPase activity in control soybean (+65%), control and diabetic DHA high dose (+39%, +53%) and diabetic DHA low dose (+131%) groups. In SN, the soybean caused a significant decrease in Na,K-ATPase activity (-26%) and still more in the diabetic group (-53%). The DHA diet induced a slight decrease in activity in control groups, whilst during diabetes, at high dose, we noted an aggravation of this decrease (-36%). Mg-ATPase activity was not modified by supplementations except for the low dose of DHA where the activity was slightly decreased in the control group (-16%). The supplementations induced multiple tissue-specific modifications in the membrane fatty acid composition of RBC and of SN homogenates. Several specific correlations have been found between variations in fatty acids amounts and Na,K-ATPase activity in these tissues but only in RBC for Mg-ATPase activity. Indeed, we observed that the variations in Na,K-ATPase activity are positively and significantly correlated with changes in the omega6/omega3 ratio in SN as well as in RBC. These data clearly show, for the first time, that the diet could modulate the Na,K-ATPase activity via the omega6/omega3 ratio in the membranes. A similar correlation was observed with Mg-ATPase activity in RBC, suggesting also a dietary regulation of the enzyme; but for the SN, this activity might be regulated by a different omega6/omega3 ratio or by another pathway. SN - 0022-2631 UR - https://www.unboundmedicine.com/medline/citation/12532275/Differential_effect_of_omega3_PUFA_supplementations_on_NaK_ATPase_and_Mg_ATPase_activities:_possible_role_of_the_membrane_omega6/omega3_ratio_ L2 - https://dx.doi.org/10.1007/s00232-002-1039-z DB - PRIME DP - Unbound Medicine ER -