Tags

Type your tag names separated by a space and hit enter

Lymphocyte subpopulations in hypertrophied adenoid in children with otitis media with effusion.
Rocz Akad Med Bialymst. 2002; 47:105-12.RA

Abstract

Otitis media with effusion (OME) usually coexist with the adenoid hypertrophy. The aim of this study was a quantitative evaluation of the lymphocyte subpopulations in hypertrophied adenoid in children with persistent (ove three months) otitis media with effusion. We used flow cytometry for study the subpopulations of the lymphocytes in the pharyngeal adenoid during otitis media in the children, alternative group consist of children with simple adenoid hypertrophy. The monoclonal antibodies selected were specific markers for T lymphocytes (CD3), T-helper cells (CD4), T-suppressor cells (CD8), B cells (CD19) and NK cells (CD56). There were also analyzed T activation markers: HLA-DR and CD25. The percentage of NK cells was significantly decreased in the group of younger children with otitis media with effusion (0.81 +/- 0.51) in comparison to older ones (1.40 +/- 0.59%). Also, in the group of younger children with adenoid hypertrophy, the NK cells percentage was significantly lower (0.85 +/- 0.37%) than in the group of older children--(1.46 +/- 0.83). In both subgroups of younger children the percentage of lymphocytes T with HLA-DR activation marker was close, but it was significantly lower in the group of older children with otitis media with effusion (4.28 +/- 3.40%) in comparison to the group of older children with adenoid hypertrophy (5.03 +/- 3.00%).

Authors+Show Affiliations

Wysocka J
Department of Pediatric Laboratory Diagnostics, Medical Academy of Bialystok, Białystok, Poland. zldpam@poczta.fm
Hassmann E
No affiliation info available
Kasprzycka E
No affiliation info available
Musiatowicz M
No affiliation info available
Lipska A
No affiliation info available

MeSH

AdenoidectomyAdenoidsAdolescentAntigens, CD19B-Lymphocyte SubsetsBiomarkersCD3 ComplexCase-Control StudiesChildChild, PreschoolCulture TechniquesFemaleFlow CytometryHumansHypertrophyKiller Cells, NaturalMaleOtitis Media with EffusionProbabilityRecurrenceSeverity of Illness IndexStatistics, NonparametricT-Lymphocyte Subsets

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12533952

Citation

Wysocka, Jolanta, et al. "Lymphocyte Subpopulations in Hypertrophied Adenoid in Children With Otitis Media With Effusion." Roczniki Akademii Medycznej W Bialymstoku (1995), vol. 47, 2002, pp. 105-12.
Wysocka J, Hassmann E, Kasprzycka E, et al. Lymphocyte subpopulations in hypertrophied adenoid in children with otitis media with effusion. Rocz Akad Med Bialymst. 2002;47:105-12.
Wysocka, J., Hassmann, E., Kasprzycka, E., Musiatowicz, M., & Lipska, A. (2002). Lymphocyte subpopulations in hypertrophied adenoid in children with otitis media with effusion. Roczniki Akademii Medycznej W Bialymstoku (1995), 47, 105-12.
Wysocka J, et al. Lymphocyte Subpopulations in Hypertrophied Adenoid in Children With Otitis Media With Effusion. Rocz Akad Med Bialymst. 2002;47:105-12. PubMed PMID: 12533952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lymphocyte subpopulations in hypertrophied adenoid in children with otitis media with effusion. AU - Wysocka,Jolanta, AU - Hassmann,Elzbieta, AU - Kasprzycka,Edwina, AU - Musiatowicz,Marcin, AU - Lipska,Alina, PY - 2003/1/22/pubmed PY - 2003/2/7/medline PY - 2003/1/22/entrez SP - 105 EP - 12 JF - Roczniki Akademii Medycznej w Bialymstoku (1995) JO - Rocz Akad Med Bialymst VL - 47 N2 - Otitis media with effusion (OME) usually coexist with the adenoid hypertrophy. The aim of this study was a quantitative evaluation of the lymphocyte subpopulations in hypertrophied adenoid in children with persistent (ove three months) otitis media with effusion. We used flow cytometry for study the subpopulations of the lymphocytes in the pharyngeal adenoid during otitis media in the children, alternative group consist of children with simple adenoid hypertrophy. The monoclonal antibodies selected were specific markers for T lymphocytes (CD3), T-helper cells (CD4), T-suppressor cells (CD8), B cells (CD19) and NK cells (CD56). There were also analyzed T activation markers: HLA-DR and CD25. The percentage of NK cells was significantly decreased in the group of younger children with otitis media with effusion (0.81 +/- 0.51) in comparison to older ones (1.40 +/- 0.59%). Also, in the group of younger children with adenoid hypertrophy, the NK cells percentage was significantly lower (0.85 +/- 0.37%) than in the group of older children--(1.46 +/- 0.83). In both subgroups of younger children the percentage of lymphocytes T with HLA-DR activation marker was close, but it was significantly lower in the group of older children with otitis media with effusion (4.28 +/- 3.40%) in comparison to the group of older children with adenoid hypertrophy (5.03 +/- 3.00%). UR - https://www.unboundmedicine.com/medline/citation/12533952/Lymphocyte_subpopulations_in_hypertrophied_adenoid_in_children_with_otitis_media_with_effusion_ DB - PRIME DP - Unbound Medicine ER -