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[Culture and characterization of human peritoneal mesothelial cells].
Hunan Yi Ke Da Xue Xue Bao. 2001 Aug 28; 26(4):321-4.HY

Abstract

OBJECTIVE

To establish a reproducible model for culture of human peritoneal mesothelial cells (HPMCs), and observe the expressions of extracellular matrix (ECM) protein and interleukin-8 (IL-8) in HPMC.

METHODS

Mesothelial cells were isolated from human omenta by trypsin EDTA disaggregation. HPMCs were identified by morphology and streptomyces protein-peroxidase (SP) method. In the same way, expressions of fibronectin, collagen type III, V and transforming growth factor beta 1 (TGF beta 1) were measured. Expression of IL-8 mRNA and FN mRNA in the HPMCs was detected by reverse transcription (RT) and polymerase chain reaction (PCR) amplification.

RESULTS

Confluent HPMCs appeared multipolar under microscope; numerous surface microvilli and an abundant endoplasmic reticulum were observed. Cultured HPMCs coexpressed cytokeratin and vimentin, and synthesized fibronectin, TGF beta 1 and collagen types III (instead of type V). Expressions of IL-8 mRNA and FN mRNA were also observed in HPMCs.

CONCLUSION

Establishment of cultured HPMCs model will provide the basis of the research in preventment of peritoneal fibrosis and roles of IL-8 in peritoneal dialysis.

Authors+Show Affiliations

Department of Nephrology, Second Xiangya Hospital, Central South University, Chiangsha 410011, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12536723

Citation

Liu, F Y., et al. "[Culture and Characterization of Human Peritoneal Mesothelial Cells]." Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University, vol. 26, no. 4, 2001, pp. 321-4.
Liu FY, Duan SB, Long ZG. [Culture and characterization of human peritoneal mesothelial cells]. Hunan Yi Ke Da Xue Xue Bao. 2001;26(4):321-4.
Liu, F. Y., Duan, S. B., & Long, Z. G. (2001). [Culture and characterization of human peritoneal mesothelial cells]. Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University, 26(4), 321-4.
Liu FY, Duan SB, Long ZG. [Culture and Characterization of Human Peritoneal Mesothelial Cells]. Hunan Yi Ke Da Xue Xue Bao. 2001 Aug 28;26(4):321-4. PubMed PMID: 12536723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Culture and characterization of human peritoneal mesothelial cells]. AU - Liu,F Y, AU - Duan,S B, AU - Long,Z G, PY - 2003/1/23/pubmed PY - 2003/6/19/medline PY - 2003/1/23/entrez SP - 321 EP - 4 JF - Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University JO - Hunan Yi Ke Da Xue Xue Bao VL - 26 IS - 4 N2 - OBJECTIVE: To establish a reproducible model for culture of human peritoneal mesothelial cells (HPMCs), and observe the expressions of extracellular matrix (ECM) protein and interleukin-8 (IL-8) in HPMC. METHODS: Mesothelial cells were isolated from human omenta by trypsin EDTA disaggregation. HPMCs were identified by morphology and streptomyces protein-peroxidase (SP) method. In the same way, expressions of fibronectin, collagen type III, V and transforming growth factor beta 1 (TGF beta 1) were measured. Expression of IL-8 mRNA and FN mRNA in the HPMCs was detected by reverse transcription (RT) and polymerase chain reaction (PCR) amplification. RESULTS: Confluent HPMCs appeared multipolar under microscope; numerous surface microvilli and an abundant endoplasmic reticulum were observed. Cultured HPMCs coexpressed cytokeratin and vimentin, and synthesized fibronectin, TGF beta 1 and collagen types III (instead of type V). Expressions of IL-8 mRNA and FN mRNA were also observed in HPMCs. CONCLUSION: Establishment of cultured HPMCs model will provide the basis of the research in preventment of peritoneal fibrosis and roles of IL-8 in peritoneal dialysis. SN - 1000-5625 UR - https://www.unboundmedicine.com/medline/citation/12536723/[Culture_and_characterization_of_human_peritoneal_mesothelial_cells]_ DB - PRIME DP - Unbound Medicine ER -