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Simultaneous determination of all polyphenols in vegetables, fruits, and teas.
J Agric Food Chem. 2003 Jan 29; 51(3):571-81.JA

Abstract

Polyphenols, which have beneficial effects on health and occur ubiquitously in plant foods, are extremely diverse. We developed a method for simultaneously determining all the polyphenols in foodstuffs, using HPLC and a photodiode array to construct a library comprising retention times, spectra of aglycons, and respective calibration curves for 100 standard chemicals. The food was homogenized in liquid nitrogen, lyophilized, extracted with 90% methanol, and subjected to HPLC without hydrolysis. The recovery was 68-92%, and the variation in reproducibility ranged between 1 and 9%. The HPLC eluted polyphenols with good resolution within 95 min in the following order: simple polyphenols, catechins, anthocyanins, glycosides of flavones, flavonols, isoflavones and flavanones, their aglycons, anthraquinones, chalcones, and theaflavins. All the polyphenols in 63 vegetables, fruits, and teas were then examined in terms of content and class. The present method offers accuracy by avoiding the decomposition of polyphenols during hydrolysis, the ability to determine aglycons separately from glycosides, and information on simple polyphenol levels simultaneously.

Authors+Show Affiliations

Department of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12537425

Citation

Sakakibara, Hiroyuki, et al. "Simultaneous Determination of All Polyphenols in Vegetables, Fruits, and Teas." Journal of Agricultural and Food Chemistry, vol. 51, no. 3, 2003, pp. 571-81.
Sakakibara H, Honda Y, Nakagawa S, et al. Simultaneous determination of all polyphenols in vegetables, fruits, and teas. J Agric Food Chem. 2003;51(3):571-81.
Sakakibara, H., Honda, Y., Nakagawa, S., Ashida, H., & Kanazawa, K. (2003). Simultaneous determination of all polyphenols in vegetables, fruits, and teas. Journal of Agricultural and Food Chemistry, 51(3), 571-81.
Sakakibara H, et al. Simultaneous Determination of All Polyphenols in Vegetables, Fruits, and Teas. J Agric Food Chem. 2003 Jan 29;51(3):571-81. PubMed PMID: 12537425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of all polyphenols in vegetables, fruits, and teas. AU - Sakakibara,Hiroyuki, AU - Honda,Yoshinori, AU - Nakagawa,Satoshi, AU - Ashida,Hitoshi, AU - Kanazawa,Kazuki, PY - 2003/1/23/pubmed PY - 2003/3/13/medline PY - 2003/1/23/entrez SP - 571 EP - 81 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 3 N2 - Polyphenols, which have beneficial effects on health and occur ubiquitously in plant foods, are extremely diverse. We developed a method for simultaneously determining all the polyphenols in foodstuffs, using HPLC and a photodiode array to construct a library comprising retention times, spectra of aglycons, and respective calibration curves for 100 standard chemicals. The food was homogenized in liquid nitrogen, lyophilized, extracted with 90% methanol, and subjected to HPLC without hydrolysis. The recovery was 68-92%, and the variation in reproducibility ranged between 1 and 9%. The HPLC eluted polyphenols with good resolution within 95 min in the following order: simple polyphenols, catechins, anthocyanins, glycosides of flavones, flavonols, isoflavones and flavanones, their aglycons, anthraquinones, chalcones, and theaflavins. All the polyphenols in 63 vegetables, fruits, and teas were then examined in terms of content and class. The present method offers accuracy by avoiding the decomposition of polyphenols during hydrolysis, the ability to determine aglycons separately from glycosides, and information on simple polyphenol levels simultaneously. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12537425/Simultaneous_determination_of_all_polyphenols_in_vegetables_fruits_and_teas_ L2 - https://doi.org/10.1021/jf020926l DB - PRIME DP - Unbound Medicine ER -