Tags

Type your tag names separated by a space and hit enter

Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants.
Infect Immun. 2003 Feb; 71(2):629-40.II

Abstract

The shdA gene is carried on a 25-kb genetic island at centisome 54 (CS54 island) of the Salmonella enterica serotype Typhimurium chromosome. In addition to shdA, the CS54 island of Salmonella serotype Typhimurium strain LT2 contains four open reading frames designated ratA, ratB, sivI, and sivH. DNA hybridization analysis revealed that the CS54 island is comprised of two regions with distinct phylogenetic distribution within the genus Salmonella. Homologues of shdA and ratB were detected only in serotypes of Salmonella enterica subsp. I. In contrast, sequences hybridizing with ratA, sivI, and sivH were present in S. enterica subsp. II and S. bongori in addition to S. enterica subsp. I. Deletion of the ratA and sivI genes did not alter the ability of Salmonella serotype Typhimurium to colonize the organs of mice. Insertional inactivation of the sivH gene resulted in defective colonization of the Peyer's patches of the terminal ileum but normal colonization of the cecum, mesenteric lymph nodes, and spleen. Deletion of the shdA gene resulted in decreased colonization of the cecum and Peyer's patches of the terminal ileum and colonization to a lesser degree in the mesenteric lymph nodes and spleen 5 days post-oral inoculation of mice. A strain containing a deletion in the ratB gene exhibited a defect for the colonization of the cecum but not of the Peyer's patches, mesenteric lymph nodes, and spleen. The shdA and ratB deletion strains exhibited a shedding defect in mice, whereas the sivH deletion strain was shed at numbers similar to the wild type. These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice.

Authors+Show Affiliations

Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University System Health Science Center, College Station 77843-1114, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12540539

Citation

Kingsley, Robert A., et al. "Molecular and Phenotypic Analysis of the CS54 Island of Salmonella Enterica Serotype Typhimurium: Identification of Intestinal Colonization and Persistence Determinants." Infection and Immunity, vol. 71, no. 2, 2003, pp. 629-40.
Kingsley RA, Humphries AD, Weening EH, et al. Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants. Infect Immun. 2003;71(2):629-40.
Kingsley, R. A., Humphries, A. D., Weening, E. H., De Zoete, M. R., Winter, S., Papaconstantinopoulou, A., Dougan, G., & Bäumler, A. J. (2003). Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants. Infection and Immunity, 71(2), 629-40.
Kingsley RA, et al. Molecular and Phenotypic Analysis of the CS54 Island of Salmonella Enterica Serotype Typhimurium: Identification of Intestinal Colonization and Persistence Determinants. Infect Immun. 2003;71(2):629-40. PubMed PMID: 12540539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and phenotypic analysis of the CS54 island of Salmonella enterica serotype typhimurium: identification of intestinal colonization and persistence determinants. AU - Kingsley,Robert A, AU - Humphries,Andrea D, AU - Weening,Eric H, AU - De Zoete,Marcel R, AU - Winter,Sebastian, AU - Papaconstantinopoulou,Anastasia, AU - Dougan,Gordon, AU - Bäumler,Andreas J, PY - 2003/1/24/pubmed PY - 2003/2/27/medline PY - 2003/1/24/entrez SP - 629 EP - 40 JF - Infection and immunity JO - Infect Immun VL - 71 IS - 2 N2 - The shdA gene is carried on a 25-kb genetic island at centisome 54 (CS54 island) of the Salmonella enterica serotype Typhimurium chromosome. In addition to shdA, the CS54 island of Salmonella serotype Typhimurium strain LT2 contains four open reading frames designated ratA, ratB, sivI, and sivH. DNA hybridization analysis revealed that the CS54 island is comprised of two regions with distinct phylogenetic distribution within the genus Salmonella. Homologues of shdA and ratB were detected only in serotypes of Salmonella enterica subsp. I. In contrast, sequences hybridizing with ratA, sivI, and sivH were present in S. enterica subsp. II and S. bongori in addition to S. enterica subsp. I. Deletion of the ratA and sivI genes did not alter the ability of Salmonella serotype Typhimurium to colonize the organs of mice. Insertional inactivation of the sivH gene resulted in defective colonization of the Peyer's patches of the terminal ileum but normal colonization of the cecum, mesenteric lymph nodes, and spleen. Deletion of the shdA gene resulted in decreased colonization of the cecum and Peyer's patches of the terminal ileum and colonization to a lesser degree in the mesenteric lymph nodes and spleen 5 days post-oral inoculation of mice. A strain containing a deletion in the ratB gene exhibited a defect for the colonization of the cecum but not of the Peyer's patches, mesenteric lymph nodes, and spleen. The shdA and ratB deletion strains exhibited a shedding defect in mice, whereas the sivH deletion strain was shed at numbers similar to the wild type. These data suggest that colonization of the murine cecum is required for efficient fecal shedding in mice. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/12540539/Molecular_and_phenotypic_analysis_of_the_CS54_island_of_Salmonella_enterica_serotype_typhimurium:_identification_of_intestinal_colonization_and_persistence_determinants_ L2 - https://journals.asm.org/doi/10.1128/IAI.71.2.629-640.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -