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Bumetanide-induced enlargement of the intercellular space in the stria vascularis requires an active Na+-K+-ATPase.
Acta Otolaryngol. 2002 Dec; 122(8):816-21.AO

Abstract

OBJECTIVE

Loop diuretics such as bumetanide and furosemide cause an acute enlargement of the intrastrial space of the stria vascularis, with an associated decline in the endocochlear DC potential (EP). The aim of this study was to determine the role played by the Na+-K+-ATPase in the bumetanide-induced enlargement of the intrastrial space, and to examine the importance of the balance between the activities of the Na+-K+-2Cl- cotransporter and the Na+-K+-ATPase to the physiological function of the stria vascularis.

MATERIAL AND METHODS

Albino guinea pigs were used in experiments involving perilymphatic perfusion, EP measurement and electron microscopy. The effects of bumetanide on the stria vascularis were examined following inhibition of the Na+-K+-ATPase by ouabain. Ouabain was administered to the perfusate and, when the EP reached 0 mV, both ouabain and bumetanide were administered.

RESULTS

Although there was no enlargement of the intrastrial space, vacuoles were apparent in marginal cells. The vacuolar change in marginal cells was similar to that caused by ouabain alone.

CONCLUSION

This study indicates that the enlargement of the intrastrial space requires not only the blockade of the Na+-K+-2Cl- cotransporter but also normal activity of the Na+-K+-ATPase, and suggests that the bumetanide-induced enlargement of the intrastrial space resulted from the imbalance between the activities of the Na+-K+-2Cl- cotransporter and the Na+-K+-ATPase.

Authors+Show Affiliations

Department of Otolaryngology, Kochi Medical School, Nankoku, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12542198

Citation

Azuma, Hiroshi, et al. "Bumetanide-induced Enlargement of the Intercellular Space in the Stria Vascularis Requires an Active Na+-K+-ATPase." Acta Oto-laryngologica, vol. 122, no. 8, 2002, pp. 816-21.
Azuma H, Takeuchi S, Higashiyama K, et al. Bumetanide-induced enlargement of the intercellular space in the stria vascularis requires an active Na+-K+-ATPase. Acta Otolaryngol. 2002;122(8):816-21.
Azuma, H., Takeuchi, S., Higashiyama, K., Ando, M., Kakigi, A., Nakahira, M., Yamakawa, K., & Takeda, T. (2002). Bumetanide-induced enlargement of the intercellular space in the stria vascularis requires an active Na+-K+-ATPase. Acta Oto-laryngologica, 122(8), 816-21.
Azuma H, et al. Bumetanide-induced Enlargement of the Intercellular Space in the Stria Vascularis Requires an Active Na+-K+-ATPase. Acta Otolaryngol. 2002;122(8):816-21. PubMed PMID: 12542198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bumetanide-induced enlargement of the intercellular space in the stria vascularis requires an active Na+-K+-ATPase. AU - Azuma,Hiroshi, AU - Takeuchi,Shunji, AU - Higashiyama,Kasumi, AU - Ando,Motonori, AU - Kakigi,Akinobu, AU - Nakahira,Mitsuhiko, AU - Yamakawa,Kazuhiro, AU - Takeda,Taizo, PY - 2003/1/25/pubmed PY - 2003/4/11/medline PY - 2003/1/25/entrez SP - 816 EP - 21 JF - Acta oto-laryngologica JO - Acta Otolaryngol. VL - 122 IS - 8 N2 - OBJECTIVE: Loop diuretics such as bumetanide and furosemide cause an acute enlargement of the intrastrial space of the stria vascularis, with an associated decline in the endocochlear DC potential (EP). The aim of this study was to determine the role played by the Na+-K+-ATPase in the bumetanide-induced enlargement of the intrastrial space, and to examine the importance of the balance between the activities of the Na+-K+-2Cl- cotransporter and the Na+-K+-ATPase to the physiological function of the stria vascularis. MATERIAL AND METHODS: Albino guinea pigs were used in experiments involving perilymphatic perfusion, EP measurement and electron microscopy. The effects of bumetanide on the stria vascularis were examined following inhibition of the Na+-K+-ATPase by ouabain. Ouabain was administered to the perfusate and, when the EP reached 0 mV, both ouabain and bumetanide were administered. RESULTS: Although there was no enlargement of the intrastrial space, vacuoles were apparent in marginal cells. The vacuolar change in marginal cells was similar to that caused by ouabain alone. CONCLUSION: This study indicates that the enlargement of the intrastrial space requires not only the blockade of the Na+-K+-2Cl- cotransporter but also normal activity of the Na+-K+-ATPase, and suggests that the bumetanide-induced enlargement of the intrastrial space resulted from the imbalance between the activities of the Na+-K+-2Cl- cotransporter and the Na+-K+-ATPase. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/12542198/Bumetanide_induced_enlargement_of_the_intercellular_space_in_the_stria_vascularis_requires_an_active_Na+_K+_ATPase_ DB - PRIME DP - Unbound Medicine ER -