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Cytochrome P450 CYP6X1 cDNAs and mRNA expression levels in three strains of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae) having different susceptibilities to pyrethroid insecticide.
Insect Mol Biol. 2003 Feb; 12(1):39-49.IM

Abstract

Three cDNAs, cloned from both pyrethroid-susceptible and -resistant strains of Lygus lineolaris, contained a 1548 nucleotide open reading frame encoding a 516 amino acid residue protein. Predicted cytochrome P450s from cDNAs were classified as the first three new members of subfamily CYP6X, CYP6X1v1 for a susceptible strain and CYP6X1v2 and CYP6X1v3 for two resistant strains. Putative cytochrome P450 CYP6X1s from L. lineolaris were highly similar (up to 42% amino acid sequence identity) to several insect CYP6s that are responsible for reduced sensitivity to pyrethroid insecticides. A total of twenty-six nucleotide substitutions were revealed between cDNAs of susceptible and resistant strains. Two nucleotide substitutions resulted in amino acid changes, Asp373 to Ala373 and Ser487 to Ala487, between susceptible and resistant strains. The resistant laboratory strain contained 2.1-fold higher cytochrome P450 mRNA per microgram total RNA than the susceptible laboratory strain. Topical treatment with 10 ng permethrin elevated cytochrome P450 mRNA levels by approximately 2-fold. The results of this study indicated that cytochrome P450 gene mutation, coupled with up-regulation, was present only in the pyrethroid resistant strains, and was possibly related to resistance development in the tarnished plant bug.

Authors+Show Affiliations

Jamie Whitten Delta States Research Center, ARS-USDA, Stoneville, Mississippi 38776, USA. yczhu@ars.usda.govNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12542634

Citation

Zhu, Y C., and G L. Snodgrass. "Cytochrome P450 CYP6X1 cDNAs and mRNA Expression Levels in Three Strains of the Tarnished Plant Bug Lygus Lineolaris (Heteroptera: Miridae) Having Different Susceptibilities to Pyrethroid Insecticide." Insect Molecular Biology, vol. 12, no. 1, 2003, pp. 39-49.
Zhu YC, Snodgrass GL. Cytochrome P450 CYP6X1 cDNAs and mRNA expression levels in three strains of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae) having different susceptibilities to pyrethroid insecticide. Insect Mol Biol. 2003;12(1):39-49.
Zhu, Y. C., & Snodgrass, G. L. (2003). Cytochrome P450 CYP6X1 cDNAs and mRNA expression levels in three strains of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae) having different susceptibilities to pyrethroid insecticide. Insect Molecular Biology, 12(1), 39-49.
Zhu YC, Snodgrass GL. Cytochrome P450 CYP6X1 cDNAs and mRNA Expression Levels in Three Strains of the Tarnished Plant Bug Lygus Lineolaris (Heteroptera: Miridae) Having Different Susceptibilities to Pyrethroid Insecticide. Insect Mol Biol. 2003;12(1):39-49. PubMed PMID: 12542634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochrome P450 CYP6X1 cDNAs and mRNA expression levels in three strains of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae) having different susceptibilities to pyrethroid insecticide. AU - Zhu,Y C, AU - Snodgrass,G L, PY - 2003/1/25/pubmed PY - 2003/4/4/medline PY - 2003/1/25/entrez SP - 39 EP - 49 JF - Insect molecular biology JO - Insect Mol Biol VL - 12 IS - 1 N2 - Three cDNAs, cloned from both pyrethroid-susceptible and -resistant strains of Lygus lineolaris, contained a 1548 nucleotide open reading frame encoding a 516 amino acid residue protein. Predicted cytochrome P450s from cDNAs were classified as the first three new members of subfamily CYP6X, CYP6X1v1 for a susceptible strain and CYP6X1v2 and CYP6X1v3 for two resistant strains. Putative cytochrome P450 CYP6X1s from L. lineolaris were highly similar (up to 42% amino acid sequence identity) to several insect CYP6s that are responsible for reduced sensitivity to pyrethroid insecticides. A total of twenty-six nucleotide substitutions were revealed between cDNAs of susceptible and resistant strains. Two nucleotide substitutions resulted in amino acid changes, Asp373 to Ala373 and Ser487 to Ala487, between susceptible and resistant strains. The resistant laboratory strain contained 2.1-fold higher cytochrome P450 mRNA per microgram total RNA than the susceptible laboratory strain. Topical treatment with 10 ng permethrin elevated cytochrome P450 mRNA levels by approximately 2-fold. The results of this study indicated that cytochrome P450 gene mutation, coupled with up-regulation, was present only in the pyrethroid resistant strains, and was possibly related to resistance development in the tarnished plant bug. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/12542634/Cytochrome_P450_CYP6X1_cDNAs_and_mRNA_expression_levels_in_three_strains_of_the_tarnished_plant_bug_Lygus_lineolaris__Heteroptera:_Miridae__having_different_susceptibilities_to_pyrethroid_insecticide_ DB - PRIME DP - Unbound Medicine ER -