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Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism.
Blood Cells Mol Dis. 2002 Nov-Dec; 29(3):356-60.BC

Abstract

Dcytb has been identified as the mammalian transplasma ferric reductase that catalyzes the reduction of ferric to ferrous iron in the process of iron absorption. Its mRNA and protein levels are up-regulated by several independent stimulators of iron absorption. Furthermore, its cDNA encodes putative binding sites for heme and ascorbic acid. Using Northern and Western blots, RT-PCR and confocal microscopy, we studied the expression and localisation of Dcytb in cell lines and tissues of CD1 mice. Dcytb expression and function were modulated by iron. Dcytb and DMT1, both predominantly localised in the apical region of the duodenum were up-regulated in iron deficiency. Dcytb, the iron regulated ferric reductase may also utilize cytoplasmic ascorbate as electron donor for transmembrane reduction of iron. Dcytb expression was found in other tissues apart from the duodenum and its regulation and functions at these other sites are of interest in iron metabolism.

Authors+Show Affiliations

Division of Life Sciences, King's College, 150 Stamford Street, London SE1 9NN, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12547225

Citation

Latunde-Dada, G O., et al. "Molecular and Functional Roles of Duodenal Cytochrome B (Dcytb) in Iron Metabolism." Blood Cells, Molecules & Diseases, vol. 29, no. 3, 2002, pp. 356-60.
Latunde-Dada GO, Van der Westhuizen J, Vulpe CD, et al. Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. Blood Cells Mol Dis. 2002;29(3):356-60.
Latunde-Dada, G. O., Van der Westhuizen, J., Vulpe, C. D., Anderson, G. J., Simpson, R. J., & McKie, A. T. (2002). Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. Blood Cells, Molecules & Diseases, 29(3), 356-60.
Latunde-Dada GO, et al. Molecular and Functional Roles of Duodenal Cytochrome B (Dcytb) in Iron Metabolism. Blood Cells Mol Dis. 2002 Nov-Dec;29(3):356-60. PubMed PMID: 12547225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. AU - Latunde-Dada,G O, AU - Van der Westhuizen,J, AU - Vulpe,C D, AU - Anderson,G J, AU - Simpson,R J, AU - McKie,A T, PY - 2003/1/28/pubmed PY - 2003/12/3/medline PY - 2003/1/28/entrez SP - 356 EP - 60 JF - Blood cells, molecules & diseases JO - Blood Cells Mol Dis VL - 29 IS - 3 N2 - Dcytb has been identified as the mammalian transplasma ferric reductase that catalyzes the reduction of ferric to ferrous iron in the process of iron absorption. Its mRNA and protein levels are up-regulated by several independent stimulators of iron absorption. Furthermore, its cDNA encodes putative binding sites for heme and ascorbic acid. Using Northern and Western blots, RT-PCR and confocal microscopy, we studied the expression and localisation of Dcytb in cell lines and tissues of CD1 mice. Dcytb expression and function were modulated by iron. Dcytb and DMT1, both predominantly localised in the apical region of the duodenum were up-regulated in iron deficiency. Dcytb, the iron regulated ferric reductase may also utilize cytoplasmic ascorbate as electron donor for transmembrane reduction of iron. Dcytb expression was found in other tissues apart from the duodenum and its regulation and functions at these other sites are of interest in iron metabolism. SN - 1079-9796 UR - https://www.unboundmedicine.com/medline/citation/12547225/Molecular_and_functional_roles_of_duodenal_cytochrome_B__Dcytb__in_iron_metabolism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1079979602905747 DB - PRIME DP - Unbound Medicine ER -