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Evaluation of genotoxicity of PCP and 2,4-D by micronucleus test in freshwater fish Channa punctatus.
Ecotoxicol Environ Saf. 2003 Jan; 54(1):25-9.EE

Abstract

An in vivo study on the genotoxic effects of pentachlorophenol (PCP) and 2,4-dichlorophenoxyacetic acid (2,4-D) was carried out with freshwater air-breathing fish Channa punctatus. The fish were exposed to three sublethal doses of PCP (0.2, 0.4, and 0.6 ppm) and 2,4-D (25, 50, and 75 ppm) by medium treatment. Micronucleated erythrocytes were sampled at intervals of 48, 72, and 96 h post-treatment. Student's t test revealed significant increases in micronucleus (MN) frequency. Maximum incidence of MN was recorded at highest concentrations and durations for both chemicals. A time- and dose-dependent response of the MN frequency for both chemicals was confirmed. PCP-treated groups had 8-10 MN per cell, specifically at higher concentrations and duration. Echinocytes were recorded at higher concentrations of 2,4-D. The study confirmed the genotoxicity of PCP and 2,4-D in this organism. PCP was found to be more toxic than 2,4-D in terms of MN induction. This assay has further potential for detecting genotoxic compounds in aquatic ecosystems.

Authors+Show Affiliations

Gene-Tox Laboratory, Department of Zoology, Division of Genetics, Aligarh Muslim University, Aligarh-202002, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12547631

Citation

Farah, M Abul, et al. "Evaluation of Genotoxicity of PCP and 2,4-D By Micronucleus Test in Freshwater Fish Channa Punctatus." Ecotoxicology and Environmental Safety, vol. 54, no. 1, 2003, pp. 25-9.
Farah MA, Ateeq B, Ali MN, et al. Evaluation of genotoxicity of PCP and 2,4-D by micronucleus test in freshwater fish Channa punctatus. Ecotoxicol Environ Saf. 2003;54(1):25-9.
Farah, M. A., Ateeq, B., Ali, M. N., & Ahmad, W. (2003). Evaluation of genotoxicity of PCP and 2,4-D by micronucleus test in freshwater fish Channa punctatus. Ecotoxicology and Environmental Safety, 54(1), 25-9.
Farah MA, et al. Evaluation of Genotoxicity of PCP and 2,4-D By Micronucleus Test in Freshwater Fish Channa Punctatus. Ecotoxicol Environ Saf. 2003;54(1):25-9. PubMed PMID: 12547631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of genotoxicity of PCP and 2,4-D by micronucleus test in freshwater fish Channa punctatus. AU - Farah,M Abul, AU - Ateeq,Bushra, AU - Ali,M Niamat, AU - Ahmad,Waseem, PY - 2003/1/28/pubmed PY - 2003/5/14/medline PY - 2003/1/28/entrez SP - 25 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 54 IS - 1 N2 - An in vivo study on the genotoxic effects of pentachlorophenol (PCP) and 2,4-dichlorophenoxyacetic acid (2,4-D) was carried out with freshwater air-breathing fish Channa punctatus. The fish were exposed to three sublethal doses of PCP (0.2, 0.4, and 0.6 ppm) and 2,4-D (25, 50, and 75 ppm) by medium treatment. Micronucleated erythrocytes were sampled at intervals of 48, 72, and 96 h post-treatment. Student's t test revealed significant increases in micronucleus (MN) frequency. Maximum incidence of MN was recorded at highest concentrations and durations for both chemicals. A time- and dose-dependent response of the MN frequency for both chemicals was confirmed. PCP-treated groups had 8-10 MN per cell, specifically at higher concentrations and duration. Echinocytes were recorded at higher concentrations of 2,4-D. The study confirmed the genotoxicity of PCP and 2,4-D in this organism. PCP was found to be more toxic than 2,4-D in terms of MN induction. This assay has further potential for detecting genotoxic compounds in aquatic ecosystems. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/12547631/Evaluation_of_genotoxicity_of_PCP_and_24_D_by_micronucleus_test_in_freshwater_fish_Channa_punctatus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147651302000374 DB - PRIME DP - Unbound Medicine ER -