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Molecular mass and chain conformation of carboxymethylated derivatives of beta-glucan from sclerotia of Pleurotus tuber-regium.
Biopolymers. 2003 Feb; 68(2):150-9.B

Abstract

Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4). The degree of substitution (DS) of CTM8 fractions was analyzed by ir and elemental analysis (EA) to be 0.3-0.68. The M(w) and the intrinsic viscosity [eta] of the CTM8 fractions were measured by size-exclusion chromatography combined with multiangle laser light scattering (SEC-MALLS), MALLS, and viscometry in phosphate buffer solution (PBS) at 37 degrees C. The dependencies of [eta] and radius of gyration <s(2)>(z) (1/2) on M(w) for the CTM8 samples were found to be [eta] = (8.82 +/- 0.03) x 10(-3) M(w)(0.78 +/- 0.04) (cm(3) g(-1)) and <s(2)>(z) (1/2) = (3.09 +/- 0.05) x 10(-3) M(w)(0.75 +/- 0.06) (nm) in the M(w) range from 3.87 x 10(4) to 53.2 x 10(4). Based on current theories for wormlike chain model, the conformational parameters of the CTM8 were obtained to be 790 (nm(-1)) for M(L), 9.6 (nm) for q, which were higher than those of the native TM8 fractions, suggesting a more extended flexible chain of CTM8 in PBS. On the whole, the CTM8 fractions showed higher antitumor activity than their corresponding TM8 fractions. In view of data from molecular parameters and bioactivity, the antitumor activity of the CTM8 fractions may be correlated to its water solubility and relatively extended chain.

Authors+Show Affiliations

Zhang M
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Zhang L
No affiliation info available
Cheung PC
No affiliation info available

MeSH

Carbohydrate ConformationGlucansIndicators and ReagentsLightMethylationMolecular WeightOligosaccharidesPlant RootsPleurotusScattering, RadiationSpectrophotometry, InfraredSpectroscopy, Fourier Transform InfraredViscosity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12548620

Citation

Zhang, Mei, et al. "Molecular Mass and Chain Conformation of Carboxymethylated Derivatives of Beta-glucan From Sclerotia of Pleurotus Tuber-regium." Biopolymers, vol. 68, no. 2, 2003, pp. 150-9.
Zhang M, Zhang L, Cheung PC. Molecular mass and chain conformation of carboxymethylated derivatives of beta-glucan from sclerotia of Pleurotus tuber-regium. Biopolymers. 2003;68(2):150-9.
Zhang, M., Zhang, L., & Cheung, P. C. (2003). Molecular mass and chain conformation of carboxymethylated derivatives of beta-glucan from sclerotia of Pleurotus tuber-regium. Biopolymers, 68(2), 150-9.
Zhang M, Zhang L, Cheung PC. Molecular Mass and Chain Conformation of Carboxymethylated Derivatives of Beta-glucan From Sclerotia of Pleurotus Tuber-regium. Biopolymers. 2003;68(2):150-9. PubMed PMID: 12548620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mass and chain conformation of carboxymethylated derivatives of beta-glucan from sclerotia of Pleurotus tuber-regium. AU - Zhang,Mei, AU - Zhang,Lina, AU - Cheung,Peter Chi Keung, PY - 2003/1/28/pubmed PY - 2003/4/18/medline PY - 2003/1/28/entrez SP - 150 EP - 9 JF - Biopolymers JO - Biopolymers VL - 68 IS - 2 N2 - Seven water-insoluble (1 --> 3)-beta-D-glucan fractions TM8-1 to TM8-7 with weight-average molecular mass M(w) ranged from 2.22 to 77.4 x 10(4) obtained from the sclerotia of Pleurotus tuber-regium were carboxymethylated to produce the water-soluble fractions CTM8-1 to CTM8-7 with M(w) ranged from 3.87 to 87.8 x 10(4). The degree of substitution (DS) of CTM8 fractions was analyzed by ir and elemental analysis (EA) to be 0.3-0.68. The M(w) and the intrinsic viscosity [eta] of the CTM8 fractions were measured by size-exclusion chromatography combined with multiangle laser light scattering (SEC-MALLS), MALLS, and viscometry in phosphate buffer solution (PBS) at 37 degrees C. The dependencies of [eta] and radius of gyration <s(2)>(z) (1/2) on M(w) for the CTM8 samples were found to be [eta] = (8.82 +/- 0.03) x 10(-3) M(w)(0.78 +/- 0.04) (cm(3) g(-1)) and <s(2)>(z) (1/2) = (3.09 +/- 0.05) x 10(-3) M(w)(0.75 +/- 0.06) (nm) in the M(w) range from 3.87 x 10(4) to 53.2 x 10(4). Based on current theories for wormlike chain model, the conformational parameters of the CTM8 were obtained to be 790 (nm(-1)) for M(L), 9.6 (nm) for q, which were higher than those of the native TM8 fractions, suggesting a more extended flexible chain of CTM8 in PBS. On the whole, the CTM8 fractions showed higher antitumor activity than their corresponding TM8 fractions. In view of data from molecular parameters and bioactivity, the antitumor activity of the CTM8 fractions may be correlated to its water solubility and relatively extended chain. SN - 0006-3525 UR - https://www.unboundmedicine.com/medline/citation/12548620/Molecular_mass_and_chain_conformation_of_carboxymethylated_derivatives_of_beta_glucan_from_sclerotia_of_Pleurotus_tuber_regium_ DB - PRIME DP - Unbound Medicine ER -