Tags

Type your tag names separated by a space and hit enter

Proteomic analysis of human bronchoalveolar lavage fluid: expression profiling of surfactant-associated protein A isomers derived from human pulmonary alveolar proteinosis using immunoaffinity detection.
Proteomics. 2003 Jan; 3(1):87-94.P

Abstract

Human bronchoalveolar lavage fluid (BALF) proteins from pulmonary alveolar proteinosis (PAP) obtained by washing the epithelial lining of the lung with phosphate-buffered saline, were separated using high resolution two-dimensional gel electrophoresis (2-DE) under denaturing and reducing conditions. By Western blotting, the proteins were transferred from polyacrylamide gel onto a chemical resilient membrane. The surfactant-associated protein A (SP-A) isomers were then identified with enhanced chemiluminescence detection (ECL) using antibody-antigen reaction. Some of the gels were treated with silver staining after 2-DE. The molecular masses of SP-A isomers in BALF from PAP ranged from 20.5 to 26, 26 to 32, and 32 to 42 kDa, respectively; and isoelectric points (pI) were in pH range of 4.5-5.4 under denaturing and reducing conditions. In the mass range of 20.5-26 kDa and pI of 4.5-5.4, there were five isomers, and in mass range of 26-32 kDa and pI of 4.5 to 5.4, there were at least eight isomers on the ECL detection film. However, in the mass range of 32-42 kDa and pI of 4.5-5.4, there were three isomers separated one from another but there was also a cluster of overlapping spots on the ECL detection film. Thus, this communication describes a characteristic 2-DE pattern of SP-A isomers in BALF from PAP as follows. (1) The five isomers of mass 20.5-26 kDa and pI of 4.5-5.4; (2) the eight isomers of mass 26-32 kDa and pI of 4.5-5.4; and (3) the three isomers of mass 32-42 kDa and pI of 4.5-5.4.

Authors+Show Affiliations

Lung Research Group, Dr. Hauner children's hospital, Ludwig-Maximilians-University of Munich, Munich, Germany. prfchang@hotmail.com

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12548638

Citation

He, Chang. "Proteomic Analysis of Human Bronchoalveolar Lavage Fluid: Expression Profiling of Surfactant-associated Protein a Isomers Derived From Human Pulmonary Alveolar Proteinosis Using Immunoaffinity Detection." Proteomics, vol. 3, no. 1, 2003, pp. 87-94.
He C. Proteomic analysis of human bronchoalveolar lavage fluid: expression profiling of surfactant-associated protein A isomers derived from human pulmonary alveolar proteinosis using immunoaffinity detection. Proteomics. 2003;3(1):87-94.
He, C. (2003). Proteomic analysis of human bronchoalveolar lavage fluid: expression profiling of surfactant-associated protein A isomers derived from human pulmonary alveolar proteinosis using immunoaffinity detection. Proteomics, 3(1), 87-94.
He C. Proteomic Analysis of Human Bronchoalveolar Lavage Fluid: Expression Profiling of Surfactant-associated Protein a Isomers Derived From Human Pulmonary Alveolar Proteinosis Using Immunoaffinity Detection. Proteomics. 2003;3(1):87-94. PubMed PMID: 12548638.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis of human bronchoalveolar lavage fluid: expression profiling of surfactant-associated protein A isomers derived from human pulmonary alveolar proteinosis using immunoaffinity detection. A1 - He,Chang, PY - 2003/1/28/pubmed PY - 2003/8/2/medline PY - 2003/1/28/entrez SP - 87 EP - 94 JF - Proteomics JO - Proteomics VL - 3 IS - 1 N2 - Human bronchoalveolar lavage fluid (BALF) proteins from pulmonary alveolar proteinosis (PAP) obtained by washing the epithelial lining of the lung with phosphate-buffered saline, were separated using high resolution two-dimensional gel electrophoresis (2-DE) under denaturing and reducing conditions. By Western blotting, the proteins were transferred from polyacrylamide gel onto a chemical resilient membrane. The surfactant-associated protein A (SP-A) isomers were then identified with enhanced chemiluminescence detection (ECL) using antibody-antigen reaction. Some of the gels were treated with silver staining after 2-DE. The molecular masses of SP-A isomers in BALF from PAP ranged from 20.5 to 26, 26 to 32, and 32 to 42 kDa, respectively; and isoelectric points (pI) were in pH range of 4.5-5.4 under denaturing and reducing conditions. In the mass range of 20.5-26 kDa and pI of 4.5-5.4, there were five isomers, and in mass range of 26-32 kDa and pI of 4.5 to 5.4, there were at least eight isomers on the ECL detection film. However, in the mass range of 32-42 kDa and pI of 4.5-5.4, there were three isomers separated one from another but there was also a cluster of overlapping spots on the ECL detection film. Thus, this communication describes a characteristic 2-DE pattern of SP-A isomers in BALF from PAP as follows. (1) The five isomers of mass 20.5-26 kDa and pI of 4.5-5.4; (2) the eight isomers of mass 26-32 kDa and pI of 4.5-5.4; and (3) the three isomers of mass 32-42 kDa and pI of 4.5-5.4. SN - 1615-9853 UR - https://www.unboundmedicine.com/medline/citation/12548638/Proteomic_analysis_of_human_bronchoalveolar_lavage_fluid:_expression_profiling_of_surfactant_associated_protein_A_isomers_derived_from_human_pulmonary_alveolar_proteinosis_using_immunoaffinity_detection_ L2 - https://doi.org/10.1002/pmic.200390013 DB - PRIME DP - Unbound Medicine ER -