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Fibre type composition of soleus and extensor digitorum longus muscles in normal female inbred Lewis rats.
Acta Histochem. 2002; 104(4):399-405.AH

Abstract

We have analysed the fibre type composition of soleus and extensor digitorum longus (EDL) muscles of normal female 4-6-month-old inbred Lewis rats. This rat strain is used in our ongoing study of the effects of thyroid hormone on myosin heavy chain (MyHC) isoform expression. On the basis of the mATPase reaction, soleus muscles contained 96.1 +/- 2.9% of type 1 fibres supplemented by 2A fibres. EDL muscles contained type 1 (5.5 +/- 1.0%), type 2A (18.8 +/- 1.7%) and type 2B (75.7 +/- 2.2%) fibres. Immunohistochemical analysis and SDS gel electrophoresis confirmed that most fibres in the soleus muscle expressed the type 1 (slow) MyHC isoform and that only a small proportion of fibres expressed the fast 2a MyHC isoform. Immunohistochemical analysis and SDS gel electrophoresis demonstrated that almost half of the 2B fibres of EDL muscles expressed the 2x/d MyHC isoform. In both muscle types, many fibres expressed more than one MyHC isoform. The content of slow fibres in the soleus muscle of female inbred Lewis rats was slightly higher than that reported for Wistar rats, but was considerably higher than that of Sprague-Dawley rats, whereas substantial differences were not found in the proportion of slow and fast fibre types in EDL muscles in these strains.

Authors+Show Affiliations

Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic. tmsoukup@biomed.cas.czNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12553710

Citation

Soukup, Tomás, et al. "Fibre Type Composition of Soleus and Extensor Digitorum Longus Muscles in Normal Female Inbred Lewis Rats." Acta Histochemica, vol. 104, no. 4, 2002, pp. 399-405.
Soukup T, Zacharová G, Smerdu V. Fibre type composition of soleus and extensor digitorum longus muscles in normal female inbred Lewis rats. Acta Histochem. 2002;104(4):399-405.
Soukup, T., Zacharová, G., & Smerdu, V. (2002). Fibre type composition of soleus and extensor digitorum longus muscles in normal female inbred Lewis rats. Acta Histochemica, 104(4), 399-405.
Soukup T, Zacharová G, Smerdu V. Fibre Type Composition of Soleus and Extensor Digitorum Longus Muscles in Normal Female Inbred Lewis Rats. Acta Histochem. 2002;104(4):399-405. PubMed PMID: 12553710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibre type composition of soleus and extensor digitorum longus muscles in normal female inbred Lewis rats. AU - Soukup,Tomás, AU - Zacharová,Gisela, AU - Smerdu,Vika, PY - 2003/1/30/pubmed PY - 2003/6/12/medline PY - 2003/1/30/entrez SP - 399 EP - 405 JF - Acta histochemica JO - Acta Histochem VL - 104 IS - 4 N2 - We have analysed the fibre type composition of soleus and extensor digitorum longus (EDL) muscles of normal female 4-6-month-old inbred Lewis rats. This rat strain is used in our ongoing study of the effects of thyroid hormone on myosin heavy chain (MyHC) isoform expression. On the basis of the mATPase reaction, soleus muscles contained 96.1 +/- 2.9% of type 1 fibres supplemented by 2A fibres. EDL muscles contained type 1 (5.5 +/- 1.0%), type 2A (18.8 +/- 1.7%) and type 2B (75.7 +/- 2.2%) fibres. Immunohistochemical analysis and SDS gel electrophoresis confirmed that most fibres in the soleus muscle expressed the type 1 (slow) MyHC isoform and that only a small proportion of fibres expressed the fast 2a MyHC isoform. Immunohistochemical analysis and SDS gel electrophoresis demonstrated that almost half of the 2B fibres of EDL muscles expressed the 2x/d MyHC isoform. In both muscle types, many fibres expressed more than one MyHC isoform. The content of slow fibres in the soleus muscle of female inbred Lewis rats was slightly higher than that reported for Wistar rats, but was considerably higher than that of Sprague-Dawley rats, whereas substantial differences were not found in the proportion of slow and fast fibre types in EDL muscles in these strains. SN - 0065-1281 UR - https://www.unboundmedicine.com/medline/citation/12553710/Fibre_type_composition_of_soleus_and_extensor_digitorum_longus_muscles_in_normal_female_inbred_Lewis_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0065-1281(04)70146-4 DB - PRIME DP - Unbound Medicine ER -