Tags

Type your tag names separated by a space and hit enter

Serotypes and Shiga toxin genotypes among Escherichia coli isolated from animals and food in Argentina and Brazil.
Vet Microbiol. 2003 Apr 29; 92(4):335-49.VM

Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains isolated from animals and food in Argentina (n=44) and Brazil (n=20) were examined and compared in regard to their phenotypic and genotypic characteristics to evaluate their pathogenic potential. The clonal relatedness of STEC O157 isolates (n=22) was established by phage typing (PT) and pulsed-field gel electrophoresis (PFGE). All O157 strains studied carried eae and enterohemorrhagic E. coli (EHEC)-hly sequences. In Argentina, these strains occurred both in cattle and meat, and 50% of them carried stx2/stx2vh-a genes, whereas in Brazil the O157 strains were isolated from animals, and most harbored the stx2vh-a sequence. At least 13 different O:H serotypes were identified among the non-O157 strains studied, with serotype O113:H21 being found in both countries. All but one non-O157 strains did not carry eae gene, but EHEC-hlyA gene was found in 85.7% of them, and the stx2 genotype was also more prevalent in Argentina than in Brazil (P<0.01), where stx1 alone or in association was most common (68.8%). One STEC strain isolated from a calf in Brazil harbored the new variant referred to as stx2-NV206. PFGE analysis showed that STEC O157 strains were grouped in four clusters. One Brazilian strain was considered possibly related (> or =80%) to Argentinean strains of cluster I. Differences in the pathogenic potential, especially in regard to serotypes and stx genotypes, were observed among the STEC strains recovered from animals and food in both countries.

Authors+Show Affiliations

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12554103

Citation

Guth, Beatriz E C., et al. "Serotypes and Shiga Toxin Genotypes Among Escherichia Coli Isolated From Animals and Food in Argentina and Brazil." Veterinary Microbiology, vol. 92, no. 4, 2003, pp. 335-49.
Guth BE, Chinen I, Miliwebsky E, et al. Serotypes and Shiga toxin genotypes among Escherichia coli isolated from animals and food in Argentina and Brazil. Vet Microbiol. 2003;92(4):335-49.
Guth, B. E., Chinen, I., Miliwebsky, E., Cerqueira, A. M., Chillemi, G., Andrade, J. R., Baschkier, A., & Rivas, M. (2003). Serotypes and Shiga toxin genotypes among Escherichia coli isolated from animals and food in Argentina and Brazil. Veterinary Microbiology, 92(4), 335-49.
Guth BE, et al. Serotypes and Shiga Toxin Genotypes Among Escherichia Coli Isolated From Animals and Food in Argentina and Brazil. Vet Microbiol. 2003 Apr 29;92(4):335-49. PubMed PMID: 12554103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotypes and Shiga toxin genotypes among Escherichia coli isolated from animals and food in Argentina and Brazil. AU - Guth,Beatriz E C, AU - Chinen,Isabel, AU - Miliwebsky,Elizabeth, AU - Cerqueira,Aloysio M F, AU - Chillemi,Germán, AU - Andrade,João R C, AU - Baschkier,Ariela, AU - Rivas,Marta, PY - 2003/1/30/pubmed PY - 2003/4/9/medline PY - 2003/1/30/entrez SP - 335 EP - 49 JF - Veterinary microbiology JO - Vet Microbiol VL - 92 IS - 4 N2 - Shiga toxin (Stx)-producing Escherichia coli (STEC) strains isolated from animals and food in Argentina (n=44) and Brazil (n=20) were examined and compared in regard to their phenotypic and genotypic characteristics to evaluate their pathogenic potential. The clonal relatedness of STEC O157 isolates (n=22) was established by phage typing (PT) and pulsed-field gel electrophoresis (PFGE). All O157 strains studied carried eae and enterohemorrhagic E. coli (EHEC)-hly sequences. In Argentina, these strains occurred both in cattle and meat, and 50% of them carried stx2/stx2vh-a genes, whereas in Brazil the O157 strains were isolated from animals, and most harbored the stx2vh-a sequence. At least 13 different O:H serotypes were identified among the non-O157 strains studied, with serotype O113:H21 being found in both countries. All but one non-O157 strains did not carry eae gene, but EHEC-hlyA gene was found in 85.7% of them, and the stx2 genotype was also more prevalent in Argentina than in Brazil (P<0.01), where stx1 alone or in association was most common (68.8%). One STEC strain isolated from a calf in Brazil harbored the new variant referred to as stx2-NV206. PFGE analysis showed that STEC O157 strains were grouped in four clusters. One Brazilian strain was considered possibly related (> or =80%) to Argentinean strains of cluster I. Differences in the pathogenic potential, especially in regard to serotypes and stx genotypes, were observed among the STEC strains recovered from animals and food in both countries. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/12554103/Serotypes_and_Shiga_toxin_genotypes_among_Escherichia_coli_isolated_from_animals_and_food_in_Argentina_and_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378113502004200 DB - PRIME DP - Unbound Medicine ER -