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Diagnosis of symptomatic and asymptomatic Trichomonas vaginalis infection by applying one tube nested PCR to vaginal discharge.
J Egypt Soc Parasitol. 1999; 29(3):1031-46.JE

Abstract

One tube nested-PCR targeting a species-specific Tv-E650 repeat family of T. vaginalis genome, was applied to vaginal discharge specimen to diagnose symptomatic and asymptomatic trichomoniasis. The test was compared with axenic culture examination, wet mount preparation and Papanicolaou stained smears. Four-hundred and fifty cases symptomatic and symptomatic were collected over two years. Out of 290 symptomatic women with cervicovaginitis and 160 asymptomatic women, 35 were culture positive for trichomoniasis. All culture positive specimens were PCR-positive giving a single product at 290 bp by agarose gel electrophoresis and recording 100% sensitivity, similar to culture examination. Among the 35 culture positive specimens, 12 were positive by wet mount and 21 were positive by Pap smear giving a 34.2% and 60% sensitivity, respectively. The standard and boiling DNA extraction methods were equally reliable but the latter was more simple, rapid and cheap. No specimens negative by PCR for trichomoniasis were positive by culture, wet mount or Pap smear. Moreover, specimens from cases with cervicovaginitis of non-trichomonal origin were negative by PCR. All samples of extracted DNA of T. vaginalis from positive culture tubes, used as positive controls, were also PCR positive at the expected product in gel, while no PCR product was detected with the negative DNA control including Chylamidia trachomatis and human DNA. It was found that the one-tube nested-PCR targeting the Tv-E650 repeat family of T. vaginalis is a simple, rapid sensitive and specific accurate method for diagnosis of symptomatic and asymptomatic vaginal trichomoniasis when applied to vaginal discharge.

Authors+Show Affiliations

Mahmoud MS
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
Abdel-Aziz SS
No affiliation info available
El-Sherif EA
No affiliation info available
Swidan KH
No affiliation info available

MeSH

AdolescentAdultAnimalsDNA, ProtozoanFemaleHumansMiddle AgedPolymerase Chain ReactionSensitivity and SpecificityTrichomonas VaginitisTrichomonas vaginalisVaginaVaginal Discharge

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

12561939

Citation

Mahmoud, M S., et al. "Diagnosis of Symptomatic and Asymptomatic Trichomonas Vaginalis Infection By Applying One Tube Nested PCR to Vaginal Discharge." Journal of the Egyptian Society of Parasitology, vol. 29, no. 3, 1999, pp. 1031-46.
Mahmoud MS, Abdel-Aziz SS, El-Sherif EA, et al. Diagnosis of symptomatic and asymptomatic Trichomonas vaginalis infection by applying one tube nested PCR to vaginal discharge. J Egypt Soc Parasitol. 1999;29(3):1031-46.
Mahmoud, M. S., Abdel-Aziz, S. S., El-Sherif, E. A., & Swidan, K. H. (1999). Diagnosis of symptomatic and asymptomatic Trichomonas vaginalis infection by applying one tube nested PCR to vaginal discharge. Journal of the Egyptian Society of Parasitology, 29(3), 1031-46.
Mahmoud MS, et al. Diagnosis of Symptomatic and Asymptomatic Trichomonas Vaginalis Infection By Applying One Tube Nested PCR to Vaginal Discharge. J Egypt Soc Parasitol. 1999;29(3):1031-46. PubMed PMID: 12561939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of symptomatic and asymptomatic Trichomonas vaginalis infection by applying one tube nested PCR to vaginal discharge. AU - Mahmoud,M S, AU - Abdel-Aziz,S S, AU - El-Sherif,E A, AU - Swidan,K H, PY - 2003/2/4/pubmed PY - 2003/2/28/medline PY - 2003/2/4/entrez SP - 1031 EP - 46 JF - Journal of the Egyptian Society of Parasitology JO - J Egypt Soc Parasitol VL - 29 IS - 3 N2 - One tube nested-PCR targeting a species-specific Tv-E650 repeat family of T. vaginalis genome, was applied to vaginal discharge specimen to diagnose symptomatic and asymptomatic trichomoniasis. The test was compared with axenic culture examination, wet mount preparation and Papanicolaou stained smears. Four-hundred and fifty cases symptomatic and symptomatic were collected over two years. Out of 290 symptomatic women with cervicovaginitis and 160 asymptomatic women, 35 were culture positive for trichomoniasis. All culture positive specimens were PCR-positive giving a single product at 290 bp by agarose gel electrophoresis and recording 100% sensitivity, similar to culture examination. Among the 35 culture positive specimens, 12 were positive by wet mount and 21 were positive by Pap smear giving a 34.2% and 60% sensitivity, respectively. The standard and boiling DNA extraction methods were equally reliable but the latter was more simple, rapid and cheap. No specimens negative by PCR for trichomoniasis were positive by culture, wet mount or Pap smear. Moreover, specimens from cases with cervicovaginitis of non-trichomonal origin were negative by PCR. All samples of extracted DNA of T. vaginalis from positive culture tubes, used as positive controls, were also PCR positive at the expected product in gel, while no PCR product was detected with the negative DNA control including Chylamidia trachomatis and human DNA. It was found that the one-tube nested-PCR targeting the Tv-E650 repeat family of T. vaginalis is a simple, rapid sensitive and specific accurate method for diagnosis of symptomatic and asymptomatic vaginal trichomoniasis when applied to vaginal discharge. SN - 1110-0583 UR - https://www.unboundmedicine.com/medline/citation/12561939/Diagnosis_of_symptomatic_and_asymptomatic_Trichomonas_vaginalis_infection_by_applying_one_tube_nested_PCR_to_vaginal_discharge_ DB - PRIME DP - Unbound Medicine ER -