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High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: implications for pathogenesis.
Virology. 2003 Jan 20; 305(2):330-8.V

Abstract

Studies of the pathogenesis of dengue hemorrhagic fever (DHF), a potentially life-threatening disease, have revealed the importance of initial high levels of virus replication. However, the possible involvement of virus during the transition from fever to defervescence, a critical stage in determining the severity of disease, has not been appreciated. Using quantitative reverse transcription-polymerase chain reaction, we examined the levels of plasma dengue viral load during both fever and defervescence periods in patients from a DEN-3 outbreak in southern Taiwan in 1998. Higher levels of plasma dengue viral RNA were found in DHF patients than in DF patients. During defervescence, while the level of plasma dengue viral RNA was undetectable in most DF patients, it remains high in all DHF patients. Using a modified immunoprecipitation assay, we demonstrated for the first time that the plasma dengue viruses persisting during defervescence were in the immune complexes for most DHF patients. These findings suggest that continued active viral replication or delay in the clearance of viremia contributes to the pathogenesis of DHF. Moreover, high levels of plasma dengue viral RNA during defervescence may serve as a disease marker for DHF.

Authors+Show Affiliations

Institute of Microbiology, College of Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan. wwang60@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12573578

Citation

Wang, Wei-Kung, et al. "High Levels of Plasma Dengue Viral Load During Defervescence in Patients With Dengue Hemorrhagic Fever: Implications for Pathogenesis." Virology, vol. 305, no. 2, 2003, pp. 330-8.
Wang WK, Chao DY, Kao CL, et al. High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: implications for pathogenesis. Virology. 2003;305(2):330-8.
Wang, W. K., Chao, D. Y., Kao, C. L., Wu, H. C., Liu, Y. C., Li, C. M., Lin, S. C., Ho, S. T., Huang, J. H., & King, C. C. (2003). High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: implications for pathogenesis. Virology, 305(2), 330-8.
Wang WK, et al. High Levels of Plasma Dengue Viral Load During Defervescence in Patients With Dengue Hemorrhagic Fever: Implications for Pathogenesis. Virology. 2003 Jan 20;305(2):330-8. PubMed PMID: 12573578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: implications for pathogenesis. AU - Wang,Wei-Kung, AU - Chao,Day-Yu, AU - Kao,Chuan-Liang, AU - Wu,Han-Chung, AU - Liu,Yung-Ching, AU - Li,Chien-Ming, AU - Lin,Shih-Chung, AU - Ho,Shih-Ting, AU - Huang,Jyh-Hsiung, AU - King,Chwan-Chuen, PY - 2003/2/8/pubmed PY - 2003/2/21/medline PY - 2003/2/8/entrez SP - 330 EP - 8 JF - Virology JO - Virology VL - 305 IS - 2 N2 - Studies of the pathogenesis of dengue hemorrhagic fever (DHF), a potentially life-threatening disease, have revealed the importance of initial high levels of virus replication. However, the possible involvement of virus during the transition from fever to defervescence, a critical stage in determining the severity of disease, has not been appreciated. Using quantitative reverse transcription-polymerase chain reaction, we examined the levels of plasma dengue viral load during both fever and defervescence periods in patients from a DEN-3 outbreak in southern Taiwan in 1998. Higher levels of plasma dengue viral RNA were found in DHF patients than in DF patients. During defervescence, while the level of plasma dengue viral RNA was undetectable in most DF patients, it remains high in all DHF patients. Using a modified immunoprecipitation assay, we demonstrated for the first time that the plasma dengue viruses persisting during defervescence were in the immune complexes for most DHF patients. These findings suggest that continued active viral replication or delay in the clearance of viremia contributes to the pathogenesis of DHF. Moreover, high levels of plasma dengue viral RNA during defervescence may serve as a disease marker for DHF. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/12573578/High_levels_of_plasma_dengue_viral_load_during_defervescence_in_patients_with_dengue_hemorrhagic_fever:_implications_for_pathogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042682202917046 DB - PRIME DP - Unbound Medicine ER -