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Renal sympathetic nerve responses to tempol in spontaneously hypertensive rats.
Hypertension. 2003 Feb; 41(2):266-73.H

Abstract

Recent studies have implicated a contribution of oxidative stress to the development of hypertension. Studies were performed to determine the effects of the superoxide dismutase (SOD) mimetic 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (Tempol) on vascular superoxide production and renal sympathetic nerve activity (RSNA) in anesthetized Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Compared with WKY rats (n=6), SHR showed a doubled vascular superoxide production, which was normalized by treatment with Tempol (3 mmol/L, n=7). In WKY rats (n=6), Tempol (30 mg/kg IV) significantly decreased mean arterial pressure (MAP) from 108+/-5 to 88+/-6 mm Hg and HR from 304+/-9 to 282+/-6 beats/min. In SHR (n=6), Tempol significantly decreased MAP from 166+/-4 to 123+/-9 mm Hg and HR from 380+/-7 to 329+/-12 beats/min. Furthermore, Tempol significantly decreased RSNA in both WKY rats and SHR. On the basis of group comparisons, the percentage decreases in MAP (-28+/-4%), HR (-16+/-3%) and integrated RSNA (-63+/-6%) in SHR were significantly greater than in WKY rats (-17+/-3%, -9+/-2%, and -30+/-4%, respectively). In SHR, changes in integrated RSNA were highly correlated with changes in MAP (r=0.85, P<0.0001) during administration of Tempol (3, 10, and 30 mg/kg IV). In both WKY rats and SHR (n=4, respectively), intracerebroventricular injection of Tempol (300 micro g/1 micro L) did not alter MAP, HR, or RSNA. Intravenous administration of a SOD inhibitor, diethyldithio-carbamic acid (30 mg/kg), significantly increased MAP, HR, and integrated RSNA in both WKY rats and SHR (n=6, respectively). These results suggest that augmented superoxide production contributes to the development of hypertension through activation of the sympathetic nervous system.

Authors+Show Affiliations

Second Department of Medicine, Kagawa Medical University, Kita-Gun, Kagawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12574093

Citation

Shokoji, Takatomi, et al. "Renal Sympathetic Nerve Responses to Tempol in Spontaneously Hypertensive Rats." Hypertension (Dallas, Tex. : 1979), vol. 41, no. 2, 2003, pp. 266-73.
Shokoji T, Nishiyama A, Fujisawa Y, et al. Renal sympathetic nerve responses to tempol in spontaneously hypertensive rats. Hypertension. 2003;41(2):266-73.
Shokoji, T., Nishiyama, A., Fujisawa, Y., Hitomi, H., Kiyomoto, H., Takahashi, N., Kimura, S., Kohno, M., & Abe, Y. (2003). Renal sympathetic nerve responses to tempol in spontaneously hypertensive rats. Hypertension (Dallas, Tex. : 1979), 41(2), 266-73.
Shokoji T, et al. Renal Sympathetic Nerve Responses to Tempol in Spontaneously Hypertensive Rats. Hypertension. 2003;41(2):266-73. PubMed PMID: 12574093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Renal sympathetic nerve responses to tempol in spontaneously hypertensive rats. AU - Shokoji,Takatomi, AU - Nishiyama,Akira, AU - Fujisawa,Yoshihide, AU - Hitomi,Hirofumi, AU - Kiyomoto,Hideyasu, AU - Takahashi,Norihiro, AU - Kimura,Shoji, AU - Kohno,Masakazu, AU - Abe,Youichi, PY - 2003/2/8/pubmed PY - 2003/3/11/medline PY - 2003/2/8/entrez SP - 266 EP - 73 JF - Hypertension (Dallas, Tex. : 1979) JO - Hypertension VL - 41 IS - 2 N2 - Recent studies have implicated a contribution of oxidative stress to the development of hypertension. Studies were performed to determine the effects of the superoxide dismutase (SOD) mimetic 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (Tempol) on vascular superoxide production and renal sympathetic nerve activity (RSNA) in anesthetized Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Compared with WKY rats (n=6), SHR showed a doubled vascular superoxide production, which was normalized by treatment with Tempol (3 mmol/L, n=7). In WKY rats (n=6), Tempol (30 mg/kg IV) significantly decreased mean arterial pressure (MAP) from 108+/-5 to 88+/-6 mm Hg and HR from 304+/-9 to 282+/-6 beats/min. In SHR (n=6), Tempol significantly decreased MAP from 166+/-4 to 123+/-9 mm Hg and HR from 380+/-7 to 329+/-12 beats/min. Furthermore, Tempol significantly decreased RSNA in both WKY rats and SHR. On the basis of group comparisons, the percentage decreases in MAP (-28+/-4%), HR (-16+/-3%) and integrated RSNA (-63+/-6%) in SHR were significantly greater than in WKY rats (-17+/-3%, -9+/-2%, and -30+/-4%, respectively). In SHR, changes in integrated RSNA were highly correlated with changes in MAP (r=0.85, P<0.0001) during administration of Tempol (3, 10, and 30 mg/kg IV). In both WKY rats and SHR (n=4, respectively), intracerebroventricular injection of Tempol (300 micro g/1 micro L) did not alter MAP, HR, or RSNA. Intravenous administration of a SOD inhibitor, diethyldithio-carbamic acid (30 mg/kg), significantly increased MAP, HR, and integrated RSNA in both WKY rats and SHR (n=6, respectively). These results suggest that augmented superoxide production contributes to the development of hypertension through activation of the sympathetic nervous system. SN - 1524-4563 UR - https://www.unboundmedicine.com/medline/citation/12574093/Renal_sympathetic_nerve_responses_to_tempol_in_spontaneously_hypertensive_rats_ L2 - http://www.ahajournals.org/doi/full/10.1161/01.hyp.0000049621.85474.cf?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -