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Imbalance between matrix metalloproteinases (MMP-9 and MMP-2) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in acute respiratory distress syndrome patients.
Crit Care Med. 2003 Feb; 31(2):536-42.CC

Abstract

OBJECTIVE

Matrix metalloproteinases (MMPs) are known to be involved in degradation of extracellular matrix. We aimed to assess the role of MMPs and their natural inhibitors (TIMPs) in the genesis and the evolution of acute respiratory distress syndrome (ARDS).

DESIGN

Prospective, clinical study.

SETTING

Intensive care unit of a university hospital.

PATIENTS

Twenty-one patients were assigned to three different groups: Group 1 patients developed ARDS that rapidly resolved in <4 days; Group 2 patients developed ARDS lasting >8 days; Group 3 (control group) patients had clinical criteria for hospital-acquired pneumonia without ARDS.

INTERVENTION

Bronchoalveolar lavages were performed on day 0 of the onset of ARDS and on days 4, 8, and 12 for unresolving ARDS. For group 3, the bronchoalveolar lavages were performed on day 0 of the pneumonia. On these bronchoalveolar lavage fluids, we measured the amount of MMP-9 and -2 and their inhibitors TIMP-1 and -2.

MEASUREMENTS AND MAIN RESULTS

The amount of MMP-9 measured by enzyme-linked immunosorbent assay was significantly lower in the bronchoalveolar lavages from patients with ARDS (group 1 and group 2) compared with the control group (p <.01) throughout the study. The ratio MMP-9/TIMP-1 was also significantly smaller and was less than one in the two ARDS groups (p <.05) compared with the control group (group 3), where this ratio was greater than one. In the second bronchoalveolar lavages, this ratio was greater than one only in the ARDS group that rapidly resolved (group 1), whereas it stayed less than one when the ARDS was lasting (group 2). Concerning the quantity of MMP-2 and the ratio MMP-2/TIMP-2, there was no statistical difference between the three groups throughout the study. Using zymography, there was no significant difference in the amounts of active and latent MMP-9 between the three groups. Moreover, no significant difference in the quantity of latent and active MMP-2 in the three groups was noted.

CONCLUSION

These results suggest that the MMP-9 level and MMP-9/TIMP-1 ratio play a role in the pathogenesis of ARDS and, namely, the imbalance between MMP-9 and TIMP-1 would participate in airway remodeling leading to either short- or long-course ARDS. The ratio MMP-9/TIMP-1 could be a predictive factor of the ARDS evolution.

Authors+Show Affiliations

INSERM U456, Université de Rennes, Service de Réanimation Chirurgicale, CHU de Rennes, Hôpital de Pontchaillou, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12576963

Citation

Lanchou, Jérôme, et al. "Imbalance Between Matrix Metalloproteinases (MMP-9 and MMP-2) and Tissue Inhibitors of Metalloproteinases (TIMP-1 and TIMP-2) in Acute Respiratory Distress Syndrome Patients." Critical Care Medicine, vol. 31, no. 2, 2003, pp. 536-42.
Lanchou J, Corbel M, Tanguy M, et al. Imbalance between matrix metalloproteinases (MMP-9 and MMP-2) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in acute respiratory distress syndrome patients. Crit Care Med. 2003;31(2):536-42.
Lanchou, J., Corbel, M., Tanguy, M., Germain, N., Boichot, E., Theret, N., Clement, B., Lagente, V., & Malledant, Y. (2003). Imbalance between matrix metalloproteinases (MMP-9 and MMP-2) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in acute respiratory distress syndrome patients. Critical Care Medicine, 31(2), 536-42.
Lanchou J, et al. Imbalance Between Matrix Metalloproteinases (MMP-9 and MMP-2) and Tissue Inhibitors of Metalloproteinases (TIMP-1 and TIMP-2) in Acute Respiratory Distress Syndrome Patients. Crit Care Med. 2003;31(2):536-42. PubMed PMID: 12576963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Imbalance between matrix metalloproteinases (MMP-9 and MMP-2) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in acute respiratory distress syndrome patients. AU - Lanchou,Jérôme, AU - Corbel,Marianne, AU - Tanguy,Michèle, AU - Germain,Noëlla, AU - Boichot,Elisabeth, AU - Theret,Nathalie, AU - Clement,Bruno, AU - Lagente,Vincent, AU - Malledant,Yannick, PY - 2003/2/11/pubmed PY - 2003/3/26/medline PY - 2003/2/11/entrez SP - 536 EP - 42 JF - Critical care medicine JO - Crit Care Med VL - 31 IS - 2 N2 - OBJECTIVE: Matrix metalloproteinases (MMPs) are known to be involved in degradation of extracellular matrix. We aimed to assess the role of MMPs and their natural inhibitors (TIMPs) in the genesis and the evolution of acute respiratory distress syndrome (ARDS). DESIGN: Prospective, clinical study. SETTING: Intensive care unit of a university hospital. PATIENTS: Twenty-one patients were assigned to three different groups: Group 1 patients developed ARDS that rapidly resolved in <4 days; Group 2 patients developed ARDS lasting >8 days; Group 3 (control group) patients had clinical criteria for hospital-acquired pneumonia without ARDS. INTERVENTION: Bronchoalveolar lavages were performed on day 0 of the onset of ARDS and on days 4, 8, and 12 for unresolving ARDS. For group 3, the bronchoalveolar lavages were performed on day 0 of the pneumonia. On these bronchoalveolar lavage fluids, we measured the amount of MMP-9 and -2 and their inhibitors TIMP-1 and -2. MEASUREMENTS AND MAIN RESULTS: The amount of MMP-9 measured by enzyme-linked immunosorbent assay was significantly lower in the bronchoalveolar lavages from patients with ARDS (group 1 and group 2) compared with the control group (p <.01) throughout the study. The ratio MMP-9/TIMP-1 was also significantly smaller and was less than one in the two ARDS groups (p <.05) compared with the control group (group 3), where this ratio was greater than one. In the second bronchoalveolar lavages, this ratio was greater than one only in the ARDS group that rapidly resolved (group 1), whereas it stayed less than one when the ARDS was lasting (group 2). Concerning the quantity of MMP-2 and the ratio MMP-2/TIMP-2, there was no statistical difference between the three groups throughout the study. Using zymography, there was no significant difference in the amounts of active and latent MMP-9 between the three groups. Moreover, no significant difference in the quantity of latent and active MMP-2 in the three groups was noted. CONCLUSION: These results suggest that the MMP-9 level and MMP-9/TIMP-1 ratio play a role in the pathogenesis of ARDS and, namely, the imbalance between MMP-9 and TIMP-1 would participate in airway remodeling leading to either short- or long-course ARDS. The ratio MMP-9/TIMP-1 could be a predictive factor of the ARDS evolution. SN - 0090-3493 UR - https://www.unboundmedicine.com/medline/citation/12576963/Imbalance_between_matrix_metalloproteinases__MMP_9_and_MMP_2__and_tissue_inhibitors_of_metalloproteinases__TIMP_1_and_TIMP_2__in_acute_respiratory_distress_syndrome_patients_ L2 - https://dx.doi.org/10.1097/01.CCM.0000048626.02184.F8 DB - PRIME DP - Unbound Medicine ER -