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Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament.
Nat Struct Biol. 2003 Mar; 10(3):182-6.NS

Abstract

In Saccharomyces cerevisiae, the Rad54 protein participates in the recombinational repair of double-strand DNA breaks together with the Rad51, Rad52, Rad55 and Rad57 proteins. In vitro, Rad54 interacts with Rad51 and stimulates DNA strand exchange promoted by Rad51 protein. Rad54 is a SWI2/SNF2-related protein that possesses double-stranded DNA-dependent ATPase activity and changes DNA topology in an ATP hydrolysis-dependent manner. Here we show that Rad54 catalyzes bidirectional nucleosome redistribution by sliding nucleosomes along DNA. Nucleosome redistribution is greatly stimulated by the Rad51 nucleoprotein filament but does not require the presence of homologous single-stranded DNA within the filament. On the basis of these data, we propose that Rad54 facilitates chromatin remodeling and, perhaps more generally, protein clearing at the homology search step of genetic recombination.

Authors+Show Affiliations

Division of Biological Sciences, Center for Genetics and Development, University of California, Davis, California 95616-8665, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12577053

Citation

Alexeev, Andrei, et al. "Rad54 Protein Possesses Chromatin-remodeling Activity Stimulated By the Rad51-ssDNA Nucleoprotein Filament." Nature Structural Biology, vol. 10, no. 3, 2003, pp. 182-6.
Alexeev A, Mazin A, Kowalczykowski SC. Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament. Nat Struct Biol. 2003;10(3):182-6.
Alexeev, A., Mazin, A., & Kowalczykowski, S. C. (2003). Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament. Nature Structural Biology, 10(3), 182-6.
Alexeev A, Mazin A, Kowalczykowski SC. Rad54 Protein Possesses Chromatin-remodeling Activity Stimulated By the Rad51-ssDNA Nucleoprotein Filament. Nat Struct Biol. 2003;10(3):182-6. PubMed PMID: 12577053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament. AU - Alexeev,Andrei, AU - Mazin,Alexander, AU - Kowalczykowski,Stephen C, PY - 2002/12/13/received PY - 2003/01/02/accepted PY - 2003/2/11/pubmed PY - 2003/7/2/medline PY - 2003/2/11/entrez SP - 182 EP - 6 JF - Nature structural biology JO - Nat Struct Biol VL - 10 IS - 3 N2 - In Saccharomyces cerevisiae, the Rad54 protein participates in the recombinational repair of double-strand DNA breaks together with the Rad51, Rad52, Rad55 and Rad57 proteins. In vitro, Rad54 interacts with Rad51 and stimulates DNA strand exchange promoted by Rad51 protein. Rad54 is a SWI2/SNF2-related protein that possesses double-stranded DNA-dependent ATPase activity and changes DNA topology in an ATP hydrolysis-dependent manner. Here we show that Rad54 catalyzes bidirectional nucleosome redistribution by sliding nucleosomes along DNA. Nucleosome redistribution is greatly stimulated by the Rad51 nucleoprotein filament but does not require the presence of homologous single-stranded DNA within the filament. On the basis of these data, we propose that Rad54 facilitates chromatin remodeling and, perhaps more generally, protein clearing at the homology search step of genetic recombination. SN - 1072-8368 UR - https://www.unboundmedicine.com/medline/citation/12577053/Rad54_protein_possesses_chromatin_remodeling_activity_stimulated_by_the_Rad51_ssDNA_nucleoprotein_filament_ L2 - https://facultyopinions.com/pubmed/12577053 DB - PRIME DP - Unbound Medicine ER -