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Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons.
Theor Appl Genet. 2002 Oct; 105(5):736-744.TA

Abstract

Hexaploid wheat (Triticum aestivum L em Thell) is derived from a complex hybridization procedure involving three diploid species carrying the A, B and D genomes, respectively. We recently isolated microsatellites from a T. tauschii library enriched for various motifs and evaluated the transferability of these markers to several diploid species carrying the A, B or D genomes. All of the primer pairs amplifying more than one locus on bread wheat and half of those giving D-genome-specific loci gave an amplification product on A-and/or B-diploid species. All of the markers giving a single amplification product for T. tauschii and no amplification on the other diploid species were D-genome-specific at the hexaploid level. The non-specific microsatellite markers (which gave an amplification product on diploid species carrying the A, B or D genome) gave either a complex amplification pattern on bread wheat (with several bands) or generated a single band which mapped to the D genome. Southern blot hybridizations with probes corresponding to the microsatellite flanking regions gave a signal on all diploid and hexaploid species, whatever the specificity of the microsatellite. The patterns observed on bread wheat were generally in accordance with those observed for diploid species, with slight rearrangements. This suggests that the specificity of microsatellite markers is probably due to mutations in microsatellite flanking regions rather than sequence elimination during polyploidization events and that genome stringency is higher at the polyploid than at the diploid level.

Authors+Show Affiliations

UMR INRA-UBP Amélioration et Santé des Plantes. Domaine de Crouël, 234 Avenue du Brézet, 63039 Clermont-Ferrand cedex 2, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12582487

Citation

Guyomarc'h, H., et al. "Studies of the Transferability of Microsatellites Derived From Triticum Tauschii to Hexaploid Wheat and to Diploid Related Species Using Amplification, Hybridization and Sequence Comparisons." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 105, no. 5, 2002, pp. 736-744.
Guyomarc'h H, Sourdille P, Edwards J, et al. Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theor Appl Genet. 2002;105(5):736-744.
Guyomarc'h, H., Sourdille, P., Edwards, J., & Bernard, M. (2002). Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 105(5), 736-744.
Guyomarc'h H, et al. Studies of the Transferability of Microsatellites Derived From Triticum Tauschii to Hexaploid Wheat and to Diploid Related Species Using Amplification, Hybridization and Sequence Comparisons. Theor Appl Genet. 2002;105(5):736-744. PubMed PMID: 12582487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. AU - Guyomarc'h,H., AU - Sourdille,P., AU - Edwards,J., AU - Bernard,M., Y1 - 2002/06/19/ PY - 2001/01/05/received PY - 2001/12/27/accepted PY - 2003/2/13/pubmed PY - 2003/2/13/medline PY - 2003/2/13/entrez SP - 736 EP - 744 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 105 IS - 5 N2 - Hexaploid wheat (Triticum aestivum L em Thell) is derived from a complex hybridization procedure involving three diploid species carrying the A, B and D genomes, respectively. We recently isolated microsatellites from a T. tauschii library enriched for various motifs and evaluated the transferability of these markers to several diploid species carrying the A, B or D genomes. All of the primer pairs amplifying more than one locus on bread wheat and half of those giving D-genome-specific loci gave an amplification product on A-and/or B-diploid species. All of the markers giving a single amplification product for T. tauschii and no amplification on the other diploid species were D-genome-specific at the hexaploid level. The non-specific microsatellite markers (which gave an amplification product on diploid species carrying the A, B or D genome) gave either a complex amplification pattern on bread wheat (with several bands) or generated a single band which mapped to the D genome. Southern blot hybridizations with probes corresponding to the microsatellite flanking regions gave a signal on all diploid and hexaploid species, whatever the specificity of the microsatellite. The patterns observed on bread wheat were generally in accordance with those observed for diploid species, with slight rearrangements. This suggests that the specificity of microsatellite markers is probably due to mutations in microsatellite flanking regions rather than sequence elimination during polyploidization events and that genome stringency is higher at the polyploid than at the diploid level. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/12582487/Studies_of_the_transferability_of_microsatellites_derived_from_Triticum_tauschii_to_hexaploid_wheat_and_to_diploid_related_species_using_amplification_hybridization_and_sequence_comparisons_ DB - PRIME DP - Unbound Medicine ER -
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