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Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat.
Theor Appl Genet. 2002 May; 104(6-7):1164-1172.TA

Abstract

Microsatellites were isolated from a Aegilops tauschii (the D-genome donor of bread wheat) library enriched for various motifs. Primers generated from the flanking region of the microsatellites were used successfully to amplify the corresponding loci in the D genome of bread wheat. Additional amplification sometimes also occurred from the A and B genomes. The majority of the microsatellites contained (GA)(n) and (GT)(n) motifs. GA and GT repeats appeared to be both more abundant in this library and more polymorphic than other types of repeats. The allele number for both types of dinucleotide repeats fitted a Poisson distribution. Deviance analysis showed that GA and GT were more polymorphic than other motifs in bread wheat. Within each motif type (di-, tri- and tetra-nucleotide repeats), repeat number has no influence on polymorphism. The microsatellites were mapped using the Triticum aestivum Courtot x Chinese Spring mapping population. A total of 100 markers was developed on this intraspecific map, mainly on the D genome. For polyploid species, isolation of microsatellites from an ancestral diploid donor seems to be an efficient way of developing markers for the corresponding genome in the polyploid plant.

Authors+Show Affiliations

UMR Amélioration et Santé des plantes, INRA-UBP, 63039 Clermont-Ferrand, Cedex 2, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12582627

Citation

Guyomarc'h, H., et al. "Characterisation of Polymorphic Microsatellite Markers From Aegilops Tauschii and Transferability to the D-genome of Bread Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 104, no. 6-7, 2002, pp. 1164-1172.
Guyomarc'h H, Sourdille P, Charmet G, et al. Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet. 2002;104(6-7):1164-1172.
Guyomarc'h, H., Sourdille, P., Charmet, G., Edwards, J., & Bernard, M. (2002). Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 104(6-7), 1164-1172.
Guyomarc'h H, et al. Characterisation of Polymorphic Microsatellite Markers From Aegilops Tauschii and Transferability to the D-genome of Bread Wheat. Theor Appl Genet. 2002;104(6-7):1164-1172. PubMed PMID: 12582627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. AU - Guyomarc'h,H., AU - Sourdille,P., AU - Charmet,G., AU - Edwards,J., AU - Bernard,M., Y1 - 2002/02/20/ PY - 2001/03/07/received PY - 2001/10/08/accepted PY - 2003/2/13/pubmed PY - 2003/2/13/medline PY - 2003/2/13/entrez SP - 1164 EP - 1172 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 104 IS - 6-7 N2 - Microsatellites were isolated from a Aegilops tauschii (the D-genome donor of bread wheat) library enriched for various motifs. Primers generated from the flanking region of the microsatellites were used successfully to amplify the corresponding loci in the D genome of bread wheat. Additional amplification sometimes also occurred from the A and B genomes. The majority of the microsatellites contained (GA)(n) and (GT)(n) motifs. GA and GT repeats appeared to be both more abundant in this library and more polymorphic than other types of repeats. The allele number for both types of dinucleotide repeats fitted a Poisson distribution. Deviance analysis showed that GA and GT were more polymorphic than other motifs in bread wheat. Within each motif type (di-, tri- and tetra-nucleotide repeats), repeat number has no influence on polymorphism. The microsatellites were mapped using the Triticum aestivum Courtot x Chinese Spring mapping population. A total of 100 markers was developed on this intraspecific map, mainly on the D genome. For polyploid species, isolation of microsatellites from an ancestral diploid donor seems to be an efficient way of developing markers for the corresponding genome in the polyploid plant. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/12582627/Characterisation_of_polymorphic_microsatellite_markers_from_Aegilops_tauschii_and_transferability_to_the_D_genome_of_bread_wheat_ L2 - https://dx.doi.org/10.1007/s00122-001-0827-7 DB - PRIME DP - Unbound Medicine ER -
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