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Laser-induced fluorescence as a powerful detection tool for capillary electrophoretic analysis of heparin/heparan sulfate disaccharides.
Biomed Chromatogr. 2003 Jan; 17(1):39-41.BC

Abstract

In quest for high sensitivities necessary for determining the disaccharide composition of heparin/heparan sulfate present in trace amounts in biologic samples, an ultrahighly sensitive capillary electrophoresis (CE) method using laser-induced fluorescence (LIF) detection was developed. Heparin/heparan sulfate-derived Delta-disaccharides were derivatized with the fluorophore 2-aminoacridone and resolved by a reversed-polarity CE method. Estimation of the limit of detection in concentration term and limit of quantitation showed that LIF detection of AMAC-derivatives of Delta-disaccharides resulted in 27-744 times higher sensitivity as compared to those detected by UV at 255 nm. These data suggest that CE-LIF is a powerful tool to quantify minute amounts of heparin/heparan sulfate disaccharides.

Authors+Show Affiliations

Department of Chemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Laboratory of Biochemistry, University of Patras, 261 10 Patras, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12583004

Citation

Militsopoulou, Maria, et al. "Laser-induced Fluorescence as a Powerful Detection Tool for Capillary Electrophoretic Analysis of Heparin/heparan Sulfate Disaccharides." Biomedical Chromatography : BMC, vol. 17, no. 1, 2003, pp. 39-41.
Militsopoulou M, Lecomte C, Bayle C, et al. Laser-induced fluorescence as a powerful detection tool for capillary electrophoretic analysis of heparin/heparan sulfate disaccharides. Biomed Chromatogr. 2003;17(1):39-41.
Militsopoulou, M., Lecomte, C., Bayle, C., Couderc, F., & Karamanos, N. K. (2003). Laser-induced fluorescence as a powerful detection tool for capillary electrophoretic analysis of heparin/heparan sulfate disaccharides. Biomedical Chromatography : BMC, 17(1), 39-41.
Militsopoulou M, et al. Laser-induced Fluorescence as a Powerful Detection Tool for Capillary Electrophoretic Analysis of Heparin/heparan Sulfate Disaccharides. Biomed Chromatogr. 2003;17(1):39-41. PubMed PMID: 12583004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laser-induced fluorescence as a powerful detection tool for capillary electrophoretic analysis of heparin/heparan sulfate disaccharides. AU - Militsopoulou,Maria, AU - Lecomte,Céline, AU - Bayle,Christopher, AU - Couderc,Francois, AU - Karamanos,Nikos K, PY - 2003/2/13/pubmed PY - 2003/8/9/medline PY - 2003/2/13/entrez SP - 39 EP - 41 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 17 IS - 1 N2 - In quest for high sensitivities necessary for determining the disaccharide composition of heparin/heparan sulfate present in trace amounts in biologic samples, an ultrahighly sensitive capillary electrophoresis (CE) method using laser-induced fluorescence (LIF) detection was developed. Heparin/heparan sulfate-derived Delta-disaccharides were derivatized with the fluorophore 2-aminoacridone and resolved by a reversed-polarity CE method. Estimation of the limit of detection in concentration term and limit of quantitation showed that LIF detection of AMAC-derivatives of Delta-disaccharides resulted in 27-744 times higher sensitivity as compared to those detected by UV at 255 nm. These data suggest that CE-LIF is a powerful tool to quantify minute amounts of heparin/heparan sulfate disaccharides. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/12583004/Laser_induced_fluorescence_as_a_powerful_detection_tool_for_capillary_electrophoretic_analysis_of_heparin/heparan_sulfate_disaccharides_ DB - PRIME DP - Unbound Medicine ER -