Tags

Type your tag names separated by a space and hit enter

Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth.
Plant Physiol 2003; 131(2):516-24PP

Abstract

Trehalose plays an important role in stress tolerance in plants. Trehalose-producing, transgenic rice (Oryza sativa) plants were generated by the introduction of a gene encoding a bifunctional fusion (TPSP) of the trehalose-6-phosphate (T-6-P) synthase (TPS) and T-6-P phosphatase (TPP) of Escherichia coli, under the control of the maize (Zea mays) ubiquitin promoter (Ubi1). The high catalytic efficiency (Seo et al., 2000) of the fusion enzyme and the single-gene engineering strategy make this an attractive candidate for high-level production of trehalose; it has the added advantage of reducing the accumulation of potentially deleterious T-6-P. The trehalose levels in leaf and seed extracts from Ubi1::TPSP plants were increased up to 1.076 mg g fresh weight(-1). This level was 200-fold higher than that of transgenic tobacco (Nicotiana tabacum) plants transformed independently with either TPS or TPP expression cassettes. The carbohydrate profiles were significantly altered in the seeds, but not in the leaves, of Ubi1::TPSP plants. It has been reported that transgenic plants with E. coli TPS and/or TPP were severely stunted and root morphology was altered. Interestingly, our Ubi1::TPSP plants showed no growth inhibition or visible phenotypic alterations despite the high-level production of trehalose. Moreover, trehalose accumulation in Ubi1::TPSP plants resulted in increased tolerance to drought, salt, and cold, as shown by chlorophyll fluorescence and growth inhibition analyses. Thus, our results suggest that trehalose acts as a global protectant against abiotic stress, and that rice is more tolerant to trehalose synthesis than dicots.

Authors+Show Affiliations

Department of Biological Science, Myongji University, Yongin 449-728, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12586876

Citation

Jang, In-Cheol, et al. "Expression of a Bifunctional Fusion of the Escherichia Coli Genes for Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase in Transgenic Rice Plants Increases Trehalose Accumulation and Abiotic Stress Tolerance Without Stunting Growth." Plant Physiology, vol. 131, no. 2, 2003, pp. 516-24.
Jang IC, Oh SJ, Seo JS, et al. Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiol. 2003;131(2):516-24.
Jang, I. C., Oh, S. J., Seo, J. S., Choi, W. B., Song, S. I., Kim, C. H., ... Kim, J. K. (2003). Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiology, 131(2), pp. 516-24.
Jang IC, et al. Expression of a Bifunctional Fusion of the Escherichia Coli Genes for Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase in Transgenic Rice Plants Increases Trehalose Accumulation and Abiotic Stress Tolerance Without Stunting Growth. Plant Physiol. 2003;131(2):516-24. PubMed PMID: 12586876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. AU - Jang,In-Cheol, AU - Oh,Se-Jun, AU - Seo,Ju-Seok, AU - Choi,Won-Bin, AU - Song,Sang Ik, AU - Kim,Chung Ho, AU - Kim,Youn Shic, AU - Seo,Hak-Soo, AU - Choi,Yang Do, AU - Nahm,Baek Hie, AU - Kim,Ju-Kon, PY - 2003/2/15/pubmed PY - 2003/5/24/medline PY - 2003/2/15/entrez SP - 516 EP - 24 JF - Plant physiology JO - Plant Physiol. VL - 131 IS - 2 N2 - Trehalose plays an important role in stress tolerance in plants. Trehalose-producing, transgenic rice (Oryza sativa) plants were generated by the introduction of a gene encoding a bifunctional fusion (TPSP) of the trehalose-6-phosphate (T-6-P) synthase (TPS) and T-6-P phosphatase (TPP) of Escherichia coli, under the control of the maize (Zea mays) ubiquitin promoter (Ubi1). The high catalytic efficiency (Seo et al., 2000) of the fusion enzyme and the single-gene engineering strategy make this an attractive candidate for high-level production of trehalose; it has the added advantage of reducing the accumulation of potentially deleterious T-6-P. The trehalose levels in leaf and seed extracts from Ubi1::TPSP plants were increased up to 1.076 mg g fresh weight(-1). This level was 200-fold higher than that of transgenic tobacco (Nicotiana tabacum) plants transformed independently with either TPS or TPP expression cassettes. The carbohydrate profiles were significantly altered in the seeds, but not in the leaves, of Ubi1::TPSP plants. It has been reported that transgenic plants with E. coli TPS and/or TPP were severely stunted and root morphology was altered. Interestingly, our Ubi1::TPSP plants showed no growth inhibition or visible phenotypic alterations despite the high-level production of trehalose. Moreover, trehalose accumulation in Ubi1::TPSP plants resulted in increased tolerance to drought, salt, and cold, as shown by chlorophyll fluorescence and growth inhibition analyses. Thus, our results suggest that trehalose acts as a global protectant against abiotic stress, and that rice is more tolerant to trehalose synthesis than dicots. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/12586876/Expression_of_a_bifunctional_fusion_of_the_Escherichia_coli_genes_for_trehalose_6_phosphate_synthase_and_trehalose_6_phosphate_phosphatase_in_transgenic_rice_plants_increases_trehalose_accumulation_and_abiotic_stress_tolerance_without_stunting_growth_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=12586876 DB - PRIME DP - Unbound Medicine ER -