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Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants.
Plant Physiol. 2003 Feb; 131(2):763-72.PP

Abstract

Expression of cytosolic and plastid acetyl-coenzyme A carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5' splice site for the first intron accumulates to its highest level in roots. Cytosolic ACCase mRNA also consists of two species, one of which is present at approximately a constant level, whereas the other accumulates to a high level in the lower sheath section. It is likely that different promoters are also responsible for the two forms of cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase mRNAs in the sheath section of the plant are similar. ACCase protein level is significantly lower in the leaf blades, in parallel with changes in the total ACCase mRNA level. Homoeologous ACCase genes show the same expression patterns and similar mRNA levels, suggesting that none of the genes was silenced or acquired new tissue specificity after polyploidization.

Authors+Show Affiliations

Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12586900

Citation

Podkowinski, Jan, et al. "Expression of Cytosolic and Plastid Acetyl-coenzyme a Carboxylase Genes in Young Wheat Plants." Plant Physiology, vol. 131, no. 2, 2003, pp. 763-72.
Podkowinski J, Jelenska J, Sirikhachornkit A, et al. Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants. Plant Physiol. 2003;131(2):763-72.
Podkowinski, J., Jelenska, J., Sirikhachornkit, A., Zuther, E., Haselkorn, R., & Gornicki, P. (2003). Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants. Plant Physiology, 131(2), 763-72.
Podkowinski J, et al. Expression of Cytosolic and Plastid Acetyl-coenzyme a Carboxylase Genes in Young Wheat Plants. Plant Physiol. 2003;131(2):763-72. PubMed PMID: 12586900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants. AU - Podkowinski,Jan, AU - Jelenska,Joanna, AU - Sirikhachornkit,Anchalee, AU - Zuther,Ellen, AU - Haselkorn,Robert, AU - Gornicki,Piotr, PY - 2003/2/15/pubmed PY - 2003/5/24/medline PY - 2003/2/15/entrez SP - 763 EP - 72 JF - Plant physiology JO - Plant Physiol VL - 131 IS - 2 N2 - Expression of cytosolic and plastid acetyl-coenzyme A carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5' splice site for the first intron accumulates to its highest level in roots. Cytosolic ACCase mRNA also consists of two species, one of which is present at approximately a constant level, whereas the other accumulates to a high level in the lower sheath section. It is likely that different promoters are also responsible for the two forms of cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase mRNAs in the sheath section of the plant are similar. ACCase protein level is significantly lower in the leaf blades, in parallel with changes in the total ACCase mRNA level. Homoeologous ACCase genes show the same expression patterns and similar mRNA levels, suggesting that none of the genes was silenced or acquired new tissue specificity after polyploidization. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/12586900/Expression_of_cytosolic_and_plastid_acetyl_coenzyme_A_carboxylase_genes_in_young_wheat_plants_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=12586900 DB - PRIME DP - Unbound Medicine ER -