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[Therapeutic angiogenesis with the use of vascular endothelial growth factor 165 gene in the myocardium of miniature swine].
Sheng Li Xue Bao. 2001 Jun; 53(3):183-7.SL

Abstract

The purpose of the present study was to examine whether adenovirus-mediated vascular endothelial growth factor l65 (VEGFl65) can enhance collateral vessel formation of coronary artery and improve regional myocardial perfusion and function. Using a miniature swine model of chronic myocardial ischemia, the replication-deficient recombinant adenovirus vector containing complementary deoxyribonucleic acid (cDNA) for human VEGFl65 (Ad-VEGFl65) or for beta-galactosidase (Ad-Gal) was administered directly into the ischemic myocardium in the left circumflex (LCX) distribution. Myocardial perfusion and function were assessed by electrocardiogram-gated single photon emission computed tomography (SPECT) imaging and collateral vessel development of coronary artery was assessed by ex vivo coronary angiography (CAG). Four weeks after Ad-VEGF165 administration, SPECT imaging demonstrated a significant reduction in ischemic area (P<0.01) and ischemic severity (P<0.01), and a substantial improvement in left ventricular ejection fraction (P<0.01) and regional wall motion in the LCX distribution (P<0.05), as compared with that of control animals and that before administration of Ad-VEGFl65. Collateral vessel development with Rentrop Grading was also significantly greater in Ad-VEGF165 animals than in the Ad-Gal control animals (P<0.05). It's concluded that Ad-VEGFl65 can induce collateral vessel development in ischemic myocardium and result in significant improvement in myocardial perfusion and function.

Authors+Show Affiliations

Department of Cardiology, the Chinese PLA General Hospital, Beijing 100853.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

12589401

Citation

Zhang, D Z., et al. "[Therapeutic Angiogenesis With the Use of Vascular Endothelial Growth Factor 165 Gene in the Myocardium of Miniature Swine]." Sheng Li Xue Bao : [Acta Physiologica Sinica], vol. 53, no. 3, 2001, pp. 183-7.
Zhang DZ, Gai LY, Chen YW, et al. [Therapeutic angiogenesis with the use of vascular endothelial growth factor 165 gene in the myocardium of miniature swine]. Sheng Li Xue Bao. 2001;53(3):183-7.
Zhang, D. Z., Gai, L. Y., Chen, Y. W., Fan, R. Y., Wen, Y. F., & Dong, W. (2001). [Therapeutic angiogenesis with the use of vascular endothelial growth factor 165 gene in the myocardium of miniature swine]. Sheng Li Xue Bao : [Acta Physiologica Sinica], 53(3), 183-7.
Zhang DZ, et al. [Therapeutic Angiogenesis With the Use of Vascular Endothelial Growth Factor 165 Gene in the Myocardium of Miniature Swine]. Sheng Li Xue Bao. 2001;53(3):183-7. PubMed PMID: 12589401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Therapeutic angiogenesis with the use of vascular endothelial growth factor 165 gene in the myocardium of miniature swine]. AU - Zhang,D Z, AU - Gai,L Y, AU - Chen,Y W, AU - Fan,R Y, AU - Wen,Y F, AU - Dong,W, PY - 2003/2/18/pubmed PY - 2003/8/30/medline PY - 2003/2/18/entrez SP - 183 EP - 7 JF - Sheng li xue bao : [Acta physiologica Sinica] JO - Sheng Li Xue Bao VL - 53 IS - 3 N2 - The purpose of the present study was to examine whether adenovirus-mediated vascular endothelial growth factor l65 (VEGFl65) can enhance collateral vessel formation of coronary artery and improve regional myocardial perfusion and function. Using a miniature swine model of chronic myocardial ischemia, the replication-deficient recombinant adenovirus vector containing complementary deoxyribonucleic acid (cDNA) for human VEGFl65 (Ad-VEGFl65) or for beta-galactosidase (Ad-Gal) was administered directly into the ischemic myocardium in the left circumflex (LCX) distribution. Myocardial perfusion and function were assessed by electrocardiogram-gated single photon emission computed tomography (SPECT) imaging and collateral vessel development of coronary artery was assessed by ex vivo coronary angiography (CAG). Four weeks after Ad-VEGF165 administration, SPECT imaging demonstrated a significant reduction in ischemic area (P<0.01) and ischemic severity (P<0.01), and a substantial improvement in left ventricular ejection fraction (P<0.01) and regional wall motion in the LCX distribution (P<0.05), as compared with that of control animals and that before administration of Ad-VEGFl65. Collateral vessel development with Rentrop Grading was also significantly greater in Ad-VEGF165 animals than in the Ad-Gal control animals (P<0.05). It's concluded that Ad-VEGFl65 can induce collateral vessel development in ischemic myocardium and result in significant improvement in myocardial perfusion and function. SN - 0371-0874 UR - https://www.unboundmedicine.com/medline/citation/12589401/[Therapeutic_angiogenesis_with_the_use_of_vascular_endothelial_growth_factor_165_gene_in_the_myocardium_of_miniature_swine]_ L2 - http://www.actaps.com.cn/paper/200103/pdf/04.pdf DB - PRIME DP - Unbound Medicine ER -