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Mkp1 and Mkp2, two MAPKAP-kinase homologues in Schizosaccharomyces pombe, interact with the MAP kinase Sty1.
Mol Genet Genomics. 2003 Feb; 268(5):585-97.MG

Abstract

Mkp1 (MAPKAP kinase Schizosaccharomyces pombe 1) and Mkp2 are two members from fission yeast of the sub-class of putative MAPK-activated protein kinases in yeasts, the other known members being Rck1 and Rck2 from Saccharomyces cerevisiae. The Mkp1 protein is readily co-immunoprecipitated with Sty1 from S. pombe extracts; Mkp2 shows a weaker interaction with Sty1. In mkp1 mutants, conjugation and meiosis proceed more readily and rapidly than in wild-type cells, in analogy to what was previously found for S. cerevisiae rck1 mutants. Conversely, overexpression of mkp1(+) delays meiosis. Mkp1 is phosphorylated in vivo in a sty1(+)-dependent manner; this modification is removed when cells are starved for nitrogen, a condition that is conducive to entry into stationary phase and meiosis. Overexpression of mkp1(+), like a sty1 mutation, also causes vegetative cells to elongate. The level of Mkp1 phosphorylation drops as cells enter mitosis. We have localised Mkp1 to the cytoplasm, excluded from the nucleus, in vegetative cells. The Mkp1 protein accumulates in zygotic asci and is concentrated within spores. The mkp2(+) gene has no noticeable impact on meiosis. Mkp2 is excluded from the nucleus in vegetative cells, and is concentrated at the septa of dividing cells. Mkp2 does not accumulate in meiotic cells.

Authors+Show Affiliations

Department of Cell and Molecular Biology, Lundberg Laboratory, Göteborg University, P.O. Box 462, 405 30, Göteborg, Sweden.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12589433

Citation

Asp, E, and P Sunnerhagen. "Mkp1 and Mkp2, Two MAPKAP-kinase Homologues in Schizosaccharomyces Pombe, Interact With the MAP Kinase Sty1." Molecular Genetics and Genomics : MGG, vol. 268, no. 5, 2003, pp. 585-97.
Asp E, Sunnerhagen P. Mkp1 and Mkp2, two MAPKAP-kinase homologues in Schizosaccharomyces pombe, interact with the MAP kinase Sty1. Mol Genet Genomics. 2003;268(5):585-97.
Asp, E., & Sunnerhagen, P. (2003). Mkp1 and Mkp2, two MAPKAP-kinase homologues in Schizosaccharomyces pombe, interact with the MAP kinase Sty1. Molecular Genetics and Genomics : MGG, 268(5), 585-97.
Asp E, Sunnerhagen P. Mkp1 and Mkp2, Two MAPKAP-kinase Homologues in Schizosaccharomyces Pombe, Interact With the MAP Kinase Sty1. Mol Genet Genomics. 2003;268(5):585-97. PubMed PMID: 12589433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mkp1 and Mkp2, two MAPKAP-kinase homologues in Schizosaccharomyces pombe, interact with the MAP kinase Sty1. AU - Asp,E, AU - Sunnerhagen,P, Y1 - 2003/01/15/ PY - 2002/07/28/received PY - 2002/11/08/accepted PY - 2003/2/18/pubmed PY - 2003/4/10/medline PY - 2003/2/18/entrez SP - 585 EP - 97 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 268 IS - 5 N2 - Mkp1 (MAPKAP kinase Schizosaccharomyces pombe 1) and Mkp2 are two members from fission yeast of the sub-class of putative MAPK-activated protein kinases in yeasts, the other known members being Rck1 and Rck2 from Saccharomyces cerevisiae. The Mkp1 protein is readily co-immunoprecipitated with Sty1 from S. pombe extracts; Mkp2 shows a weaker interaction with Sty1. In mkp1 mutants, conjugation and meiosis proceed more readily and rapidly than in wild-type cells, in analogy to what was previously found for S. cerevisiae rck1 mutants. Conversely, overexpression of mkp1(+) delays meiosis. Mkp1 is phosphorylated in vivo in a sty1(+)-dependent manner; this modification is removed when cells are starved for nitrogen, a condition that is conducive to entry into stationary phase and meiosis. Overexpression of mkp1(+), like a sty1 mutation, also causes vegetative cells to elongate. The level of Mkp1 phosphorylation drops as cells enter mitosis. We have localised Mkp1 to the cytoplasm, excluded from the nucleus, in vegetative cells. The Mkp1 protein accumulates in zygotic asci and is concentrated within spores. The mkp2(+) gene has no noticeable impact on meiosis. Mkp2 is excluded from the nucleus in vegetative cells, and is concentrated at the septa of dividing cells. Mkp2 does not accumulate in meiotic cells. SN - 1617-4615 UR - https://www.unboundmedicine.com/medline/citation/12589433/Mkp1_and_Mkp2_two_MAPKAP_kinase_homologues_in_Schizosaccharomyces_pombe_interact_with_the_MAP_kinase_Sty1_ L2 - https://doi.org/10.1007/s00438-002-0786-y DB - PRIME DP - Unbound Medicine ER -