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The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants.
Funct Integr Genomics. 2003 Mar; 3(1-2):76-85.FI

Abstract

Wheat and barley contain at least four classes of starch synthases in the endosperm, granule bound starch synthase I (GBSSI) and starch synthases I, II and III (SSI, SSII, SSIII). In this work, SSII in barley is shown to be associated with the starch granule by using antibodies. A cDNA from barley encoding SSII and the genes for SSII from barley and Aegilops tauschii (A. tauschii, the D genome donor to wheat) are characterised. Fluorescent in situ hybridisation (FISH) and PCR were used to localise the wheat SSII gene to the short arm of chromosome 7, showing synteny with the location of the rice SSII gene to the short arm of chromosome 6. Comparison of the genes encoding SSII of A. tauschii, barley and Arabidopsis showed a conserved exon-intron structure although the size of the introns varied considerably. Extending such comparison between the genes encoding starch synthases (GBSSI, SSI, SSII and SSIII) from A. tauschii and Arabidopsis showed that the exon-intron structures are essentially conserved. Separate and distinct genes for the individual starch synthases therefore existed before the separation of monocotyledons and dicotyledons.

Authors+Show Affiliations

Li Z
CSIRO Division of Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia. zhongyi.li@csiro.au
Sun F
No affiliation info available
Xu S
No affiliation info available
Chu X
No affiliation info available
Mukai Y
No affiliation info available
Yamamoto M
No affiliation info available
Ali S
No affiliation info available
Rampling L
No affiliation info available
Kosar-Hashemi B
No affiliation info available
Rahman S
No affiliation info available
Morell MK
No affiliation info available

MeSH

Base SequenceDNA, ComplementaryExonsGene LibraryGlycogen SynthaseHordeumIn Situ Hybridization, FluorescenceIntronsModels, GeneticMolecular Sequence DataPeptidesPhylogenyPlant ProteinsPolymerase Chain ReactionRNARNA, MessengerSequence Analysis, DNASilver StainingStarch SynthaseTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12590345

Citation

Li, Zhongyi, et al. "The Structural Organisation of the Gene Encoding Class II Starch Synthase of Wheat and Barley and the Evolution of the Genes Encoding Starch Synthases in Plants." Functional & Integrative Genomics, vol. 3, no. 1-2, 2003, pp. 76-85.
Li Z, Sun F, Xu S, et al. The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants. Funct Integr Genomics. 2003;3(1-2):76-85.
Li, Z., Sun, F., Xu, S., Chu, X., Mukai, Y., Yamamoto, M., Ali, S., Rampling, L., Kosar-Hashemi, B., Rahman, S., & Morell, M. K. (2003). The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants. Functional & Integrative Genomics, 3(1-2), 76-85.
Li Z, et al. The Structural Organisation of the Gene Encoding Class II Starch Synthase of Wheat and Barley and the Evolution of the Genes Encoding Starch Synthases in Plants. Funct Integr Genomics. 2003;3(1-2):76-85. PubMed PMID: 12590345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The structural organisation of the gene encoding class II starch synthase of wheat and barley and the evolution of the genes encoding starch synthases in plants. AU - Li,Zhongyi, AU - Sun,Fei, AU - Xu,Shoumin, AU - Chu,Xiusheng, AU - Mukai,Y, AU - Yamamoto,M, AU - Ali,Shahjahan, AU - Rampling,Lynette, AU - Kosar-Hashemi,Behjat, AU - Rahman,Sadequr, AU - Morell,Matthew K, Y1 - 2002/08/14/ PY - 2002/04/29/received PY - 2002/07/10/accepted PY - 2003/2/19/pubmed PY - 2003/9/30/medline PY - 2003/2/19/entrez SP - 76 EP - 85 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 3 IS - 1-2 N2 - Wheat and barley contain at least four classes of starch synthases in the endosperm, granule bound starch synthase I (GBSSI) and starch synthases I, II and III (SSI, SSII, SSIII). In this work, SSII in barley is shown to be associated with the starch granule by using antibodies. A cDNA from barley encoding SSII and the genes for SSII from barley and Aegilops tauschii (A. tauschii, the D genome donor to wheat) are characterised. Fluorescent in situ hybridisation (FISH) and PCR were used to localise the wheat SSII gene to the short arm of chromosome 7, showing synteny with the location of the rice SSII gene to the short arm of chromosome 6. Comparison of the genes encoding SSII of A. tauschii, barley and Arabidopsis showed a conserved exon-intron structure although the size of the introns varied considerably. Extending such comparison between the genes encoding starch synthases (GBSSI, SSI, SSII and SSIII) from A. tauschii and Arabidopsis showed that the exon-intron structures are essentially conserved. Separate and distinct genes for the individual starch synthases therefore existed before the separation of monocotyledons and dicotyledons. SN - 1438-793X UR - https://www.unboundmedicine.com/medline/citation/12590345/The_structural_organisation_of_the_gene_encoding_class_II_starch_synthase_of_wheat_and_barley_and_the_evolution_of_the_genes_encoding_starch_synthases_in_plants_ DB - PRIME DP - Unbound Medicine ER -