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Application of tandem mass spectrometry combined with gas chromatography and headspace solid-phase dynamic extraction for the determination of drugs of abuse in hair samples.
Rapid Commun Mass Spectrom. 2003; 17(5):472-8.RC

Abstract

A new method combination, headspace solid-phase dynamic extraction coupled with gas chromatography/tandem mass spectrometry (HS-SPDE/GC/MS/MS), is introduced to determine drugs of abuse in hair samples. This highly automated procedure utilizes SPDE for pre-concentration and on-coating derivatization as well as GC and triple quadrupole MS/MS for selective and sensitive detection. All these steps, apart from washing and cutting of the hair samples, are performed without manual intervention on a robot-like autosampler.SPDE is a solventless extraction technique related to solid-phase microextraction (SPME). The analytes are absorbed from the sample headspace directly into a hollow needle with an internal coating of polydimethylsiloxane by repeated aspirate/dispense cycles.The HS-SPDE/GC/MS/MS procedure was applied to the analysis of methadone, the trimethylsilyl derivatives of cannabinoids and the trifluoroacetyl derivatives of amphetamines and designer drugs. The method was shown to be sensitive with detection limits between 6 and 52 pg/mg hair matrix and precision between 0.4 and 7.8% by the use of an internal standard technique. Linearity was obtained from 0.1-20 ng/mg with coefficients of correlation between 0.995 and 0.999. Compared with conventional methods of hair analysis, HS-SPDE/GC/MS/MS is easier to use, substantially faster, with the degree of sensitivity and reproducibility demanded in clinical and forensic toxicology. The main advantage of the SPDE technique in relation to SPME is the robustness of the capillary.

Authors+Show Affiliations

Institute of Legal Medicine, University of Bonn, Germany. lachenmeier@web.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12590396

Citation

Lachenmeier, Dirk W., et al. "Application of Tandem Mass Spectrometry Combined With Gas Chromatography and Headspace Solid-phase Dynamic Extraction for the Determination of Drugs of Abuse in Hair Samples." Rapid Communications in Mass Spectrometry : RCM, vol. 17, no. 5, 2003, pp. 472-8.
Lachenmeier DW, Kroener L, Musshoff F, et al. Application of tandem mass spectrometry combined with gas chromatography and headspace solid-phase dynamic extraction for the determination of drugs of abuse in hair samples. Rapid Commun Mass Spectrom. 2003;17(5):472-8.
Lachenmeier, D. W., Kroener, L., Musshoff, F., & Madea, B. (2003). Application of tandem mass spectrometry combined with gas chromatography and headspace solid-phase dynamic extraction for the determination of drugs of abuse in hair samples. Rapid Communications in Mass Spectrometry : RCM, 17(5), 472-8.
Lachenmeier DW, et al. Application of Tandem Mass Spectrometry Combined With Gas Chromatography and Headspace Solid-phase Dynamic Extraction for the Determination of Drugs of Abuse in Hair Samples. Rapid Commun Mass Spectrom. 2003;17(5):472-8. PubMed PMID: 12590396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of tandem mass spectrometry combined with gas chromatography and headspace solid-phase dynamic extraction for the determination of drugs of abuse in hair samples. AU - Lachenmeier,Dirk W, AU - Kroener,Lars, AU - Musshoff,Frank, AU - Madea,Burkhard, PY - 2003/2/19/pubmed PY - 2003/4/10/medline PY - 2003/2/19/entrez SP - 472 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 17 IS - 5 N2 - A new method combination, headspace solid-phase dynamic extraction coupled with gas chromatography/tandem mass spectrometry (HS-SPDE/GC/MS/MS), is introduced to determine drugs of abuse in hair samples. This highly automated procedure utilizes SPDE for pre-concentration and on-coating derivatization as well as GC and triple quadrupole MS/MS for selective and sensitive detection. All these steps, apart from washing and cutting of the hair samples, are performed without manual intervention on a robot-like autosampler.SPDE is a solventless extraction technique related to solid-phase microextraction (SPME). The analytes are absorbed from the sample headspace directly into a hollow needle with an internal coating of polydimethylsiloxane by repeated aspirate/dispense cycles.The HS-SPDE/GC/MS/MS procedure was applied to the analysis of methadone, the trimethylsilyl derivatives of cannabinoids and the trifluoroacetyl derivatives of amphetamines and designer drugs. The method was shown to be sensitive with detection limits between 6 and 52 pg/mg hair matrix and precision between 0.4 and 7.8% by the use of an internal standard technique. Linearity was obtained from 0.1-20 ng/mg with coefficients of correlation between 0.995 and 0.999. Compared with conventional methods of hair analysis, HS-SPDE/GC/MS/MS is easier to use, substantially faster, with the degree of sensitivity and reproducibility demanded in clinical and forensic toxicology. The main advantage of the SPDE technique in relation to SPME is the robustness of the capillary. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/12590396/Application_of_tandem_mass_spectrometry_combined_with_gas_chromatography_and_headspace_solid_phase_dynamic_extraction_for_the_determination_of_drugs_of_abuse_in_hair_samples_ L2 - https://doi.org/10.1002/rcm.945 DB - PRIME DP - Unbound Medicine ER -