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Interactions of 5-HT2 receptor agonists with acetylcholine in spinal analgesic mechanisms in rats with neuropathic pain.
Brain Res. 2003 Mar 07; 965(1-2):114-20.BR

Abstract

Serotonin type 2 (5-HT(2)) receptors reportedly inhibit neuropathic pain in the spinal cord, but little is known about how spinal 5-HT(2) receptors might act against such abnormal sensitivity. We examined whether the cholinergic and tachykinin systems were involved in the antiallodynic effect of intrathecally administered 5-HT(2) receptor agonists in rats with nerve injury. Allodynia was produced by tight ligation of the left L5 and L6 spinal nerves, and determined by applying von Frey hairs to the left hindpaw. Effects of intrathecal pretreatment with 5-HT(2) receptor antagonists (ketanserin and RS-102221), muscarinic receptor antagonists (atropine and scopolamine), a choline uptake blocker (hemicholium-3), and an NK(1) receptor antagonist (L-706336) were assessed in rats subsequently given a 100- micro g intrathecal dose of a 5-HT(2) receptor agonist either alpha-methyl-5-HT or iododimethoxy aminopropane (DOI). Antiallodynic effects of 5-HT(2) receptor agonists were attenuated by the 5-HT(2A) receptor antagonist ketanserin (30 micro g), but not by the 5-HT(2C) receptor antagonist RS-102221 (40 micro g). Muscarinic receptor antagonists (30 micro g each), the choline uptake blocker (10 micro g), and the NK(1) receptor antagonist (30 micro g) also inhibited the antiallodynic effects of 5-HT(2) receptor agonists. Antiallodynic effects of intrathecally administered 5-HT(2) receptor agonists may be mediated by spinal release of acetylcholine induced via 5-HT(2A) and NK(1) receptors.

Authors+Show Affiliations

Department of Anesthesiology and Reanimatology, Gunma University School of Medicine, 3-39-22, Showa-machi, Maebashi 371-8511, Japan. hobata@qf7.so-net.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12591127

Citation

Obata, Hideaki, et al. "Interactions of 5-HT2 Receptor Agonists With Acetylcholine in Spinal Analgesic Mechanisms in Rats With Neuropathic Pain." Brain Research, vol. 965, no. 1-2, 2003, pp. 114-20.
Obata H, Saito S, Sasaki M, et al. Interactions of 5-HT2 receptor agonists with acetylcholine in spinal analgesic mechanisms in rats with neuropathic pain. Brain Res. 2003;965(1-2):114-20.
Obata, H., Saito, S., Sasaki, M., & Goto, F. (2003). Interactions of 5-HT2 receptor agonists with acetylcholine in spinal analgesic mechanisms in rats with neuropathic pain. Brain Research, 965(1-2), 114-20.
Obata H, et al. Interactions of 5-HT2 Receptor Agonists With Acetylcholine in Spinal Analgesic Mechanisms in Rats With Neuropathic Pain. Brain Res. 2003 Mar 7;965(1-2):114-20. PubMed PMID: 12591127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interactions of 5-HT2 receptor agonists with acetylcholine in spinal analgesic mechanisms in rats with neuropathic pain. AU - Obata,Hideaki, AU - Saito,Shigeru, AU - Sasaki,Masayuki, AU - Goto,Fumio, PY - 2003/2/20/pubmed PY - 2003/5/2/medline PY - 2003/2/20/entrez SP - 114 EP - 20 JF - Brain research JO - Brain Res VL - 965 IS - 1-2 N2 - Serotonin type 2 (5-HT(2)) receptors reportedly inhibit neuropathic pain in the spinal cord, but little is known about how spinal 5-HT(2) receptors might act against such abnormal sensitivity. We examined whether the cholinergic and tachykinin systems were involved in the antiallodynic effect of intrathecally administered 5-HT(2) receptor agonists in rats with nerve injury. Allodynia was produced by tight ligation of the left L5 and L6 spinal nerves, and determined by applying von Frey hairs to the left hindpaw. Effects of intrathecal pretreatment with 5-HT(2) receptor antagonists (ketanserin and RS-102221), muscarinic receptor antagonists (atropine and scopolamine), a choline uptake blocker (hemicholium-3), and an NK(1) receptor antagonist (L-706336) were assessed in rats subsequently given a 100- micro g intrathecal dose of a 5-HT(2) receptor agonist either alpha-methyl-5-HT or iododimethoxy aminopropane (DOI). Antiallodynic effects of 5-HT(2) receptor agonists were attenuated by the 5-HT(2A) receptor antagonist ketanserin (30 micro g), but not by the 5-HT(2C) receptor antagonist RS-102221 (40 micro g). Muscarinic receptor antagonists (30 micro g each), the choline uptake blocker (10 micro g), and the NK(1) receptor antagonist (30 micro g) also inhibited the antiallodynic effects of 5-HT(2) receptor agonists. Antiallodynic effects of intrathecally administered 5-HT(2) receptor agonists may be mediated by spinal release of acetylcholine induced via 5-HT(2A) and NK(1) receptors. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/12591127/Interactions_of_5_HT2_receptor_agonists_with_acetylcholine_in_spinal_analgesic_mechanisms_in_rats_with_neuropathic_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006899302041458 DB - PRIME DP - Unbound Medicine ER -