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Complex regulation of the Bacillus subtilis aconitase gene.
J Bacteriol. 2003 Mar; 185(5):1672-80.JB

Abstract

The roles of the CcpC, CodY, and AbrB proteins in regulation of the Bacillus subtilis aconitase (citB) gene were found to be distinct and to vary with the conditions and phase of growth. CcpC, a citrate-inhibited repressor that is the primary factor regulating citB expression in minimal-glucose-glutamine medium, also contributed to repression of citB during exponential-phase growth in broth medium. A null mutation in codY had no effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mutations. However, a codY mutation slightly relieved repression during exponential growth in broth medium and completely derepressed citB expression when combined with a ccpC mutation. An abrB mutation led to decreased expression of citB during stationary phase in both broth and minimal medium. All three proteins bound in vitro to specific and partially overlapping sites within the citB regulatory region. Interaction of CcpC and CodY with the citB promoter region was partially competitive.

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Authors+Show Affiliations

Kim HJ
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Kim SI
No affiliation info available
Ratnayake-Lecamwasam M
No affiliation info available
Tachikawa K
No affiliation info available
Sonenshein AL
No affiliation info available
Strauch M
No affiliation info available

MeSH

Aconitate HydrataseBacillus subtilisBacterial ProteinsCell DivisionCitratesCulture MediaDNA-Binding ProteinsGene Expression Regulation, BacterialMutationPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidRepressor ProteinsTranscription Factors

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12591885

Citation

Kim, Hyun-Jin, et al. "Complex Regulation of the Bacillus Subtilis Aconitase Gene." Journal of Bacteriology, vol. 185, no. 5, 2003, pp. 1672-80.
Kim HJ, Kim SI, Ratnayake-Lecamwasam M, et al. Complex regulation of the Bacillus subtilis aconitase gene. J Bacteriol. 2003;185(5):1672-80.
Kim, H. J., Kim, S. I., Ratnayake-Lecamwasam, M., Tachikawa, K., Sonenshein, A. L., & Strauch, M. (2003). Complex regulation of the Bacillus subtilis aconitase gene. Journal of Bacteriology, 185(5), 1672-80.
Kim HJ, et al. Complex Regulation of the Bacillus Subtilis Aconitase Gene. J Bacteriol. 2003;185(5):1672-80. PubMed PMID: 12591885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complex regulation of the Bacillus subtilis aconitase gene. AU - Kim,Hyun-Jin, AU - Kim,Sam-In, AU - Ratnayake-Lecamwasam,Manoja, AU - Tachikawa,Kiyoshi, AU - Sonenshein,Abraham L, AU - Strauch,Mark, PY - 2003/2/20/pubmed PY - 2003/3/26/medline PY - 2003/2/20/entrez SP - 1672 EP - 80 JF - Journal of bacteriology JO - J Bacteriol VL - 185 IS - 5 N2 - The roles of the CcpC, CodY, and AbrB proteins in regulation of the Bacillus subtilis aconitase (citB) gene were found to be distinct and to vary with the conditions and phase of growth. CcpC, a citrate-inhibited repressor that is the primary factor regulating citB expression in minimal-glucose-glutamine medium, also contributed to repression of citB during exponential-phase growth in broth medium. A null mutation in codY had no effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mutations. However, a codY mutation slightly relieved repression during exponential growth in broth medium and completely derepressed citB expression when combined with a ccpC mutation. An abrB mutation led to decreased expression of citB during stationary phase in both broth and minimal medium. All three proteins bound in vitro to specific and partially overlapping sites within the citB regulatory region. Interaction of CcpC and CodY with the citB promoter region was partially competitive. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/12591885/Complex_regulation_of_the_Bacillus_subtilis_aconitase_gene_ DB - PRIME DP - Unbound Medicine ER -